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[187666_at]

Description F47D12.3 /REP_DB=WormBase Gene ID /WP=CE01947 /TR=SW:Q09389 /GB=AAK20065.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 34      6grp_high1_10_10 > 43      4grp_highest_8_8 > 0      4grp_high_8_8 > 45      6grp_highest_nonorm > 71      6grp_total > 30      4grp_total > 51      6grp_highest_sub > 02_03      6grp_high1_sub > 02      6grp_total_sub > Hat      6grp_rule_based > S2      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 385
1 2 3 4 range
A 366.1
(0.2)
P 		477.2
(3)
P 		376.4
(0.4)
P 		240.1
(-3)
P 		237
B 297.5
(-1.5)
P 		536.1
(3)
P 		450.8
(1.4)
P 		215.5
(-3)
P 		321
C 332.3
(-1.9)
P 		600.8
(3)
P 		409.7
(-0.5)
P 		270.8
(-3)
P 		330
avr 332
(-1.2) 		538
(3) 		412.3
(0.5) 		242.1
(-3) 		296

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 329
1 2 3 4 5 6 range
A 282.1
(-1.3)
P 		505.1
(3)
P 		471.2
(2.3)
P 		416.8
(1.3)
P 		270.5
(-1.5)
P 		195.6
(-3)
P 		310
B 284.3
(-1.8)
P 		508.5
(2.7)
P 		524.9
(3)
P 		472.2
(1.9)
P 		224.7
(-3)
P 		228.5
(-2.9)
P 		300
C 229.6
(-3)
P 		524.6
(3)
P 		435.6
(1.2)
P 		386.6
(0.2)
P 		259.1
(-2.4)
P 		268.8
(-2.2)
P 		295
avr 265.3
(-2.3) 		512.7
(3) 		477.2
(2.2) 		425.2
(1.1) 		251.4
(-2.6) 		231
(-3) 		282
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 138
1 2 3 4 range
A 133.3
(3)
P 		103.6
(1.2)
P 		98.4
(0.9)
P 		35.1
(-3)
P 		98
B 94.9
(0.4)
P 		132.4
(3)
P 		104.5
(1.1)
P 		45.9
(-3)
P 		87
C 110
(0)
P 		172.7
(3)
P 		142.8
(1.6)
P 		45.7
(-3)
P 		127
avr 112.7
(1.5) 		136.2
(3) 		115.2
(1.7) 		42.2
(-3) 		94

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 132
1 2 3 4 5 6 range
A 52
(-2.8)
P 		179.6
(3)
P 		102.6
(-0.5)
P 		105.7
(-0.4)
P 		81.6
(-1.5)
P 		47.6
(-3)
P 		132
B 98.9
(-2)
P 		169.3
(3)
P 		127.5
(0)
P 		125.1
(-0.2)
P 		91.3
(-2.6)
P 		85.2
(-3)
P 		84
C 85.1
(-2.5)
P 		160.1
(3)
P 		110.9
(-0.6)
P 		77.7
(-3)
P 		83.8
(-2.6)
P 		90
(-2.1)
P 		82
avr 78.7
(-2.7) 		169.7
(3) 		113.7
(-0.5) 		102.8
(-1.2) 		85.6
(-2.3) 		74.3
(-3) 		95
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187666_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8040
Sequence Type Exemplar sequence
Representative Public ID F47D12.3 NCBI
Target Description F47D12.3 /REP_DB=WormBase Gene ID /WP=CE01947 /TR=SW:Q09389 /GB=AAK20065.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065980(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065980 NCBI Caenorhabditis elegans F47D12.3 (F47D12.3) mRNA, complete cds. 11/11 None
SNAP00000002705 ENSEMBL cdna:SNAP chromosome:CEL140:III:6298432:6299264:1 11/11 None
GENEFINDER00000002714 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:6298432:6299264:1 11/11 None
F47D12.3 ENSEMBL cdna:known chromosome:CEL140:III:6298432:6299264:1 gene:F47D12.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:6298430-6299263(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F47D12.3
Entrez Gene 185932 Entrez gene
SwissProt Q09389 EMBL-EBI
WormBase CE01947 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6813 potassium ion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
8076 voltage-gated potassium channel complex inferred from electronic annotation QuickGO AmiGO
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5249 voltage-gated potassium channel activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK20065 Hypothetical protein F47D12.3 [Caenorhabditis elegans] ref|NP_498381.1| F47D12.3 [Caenorhabditis elegans] sp|Q09389|YR43_CAEEL Hypothetical protein F47D12.3 in chromosome III 2.0E-78
blast CAE70718 Hypothetical protein CBG17460 [Caenorhabditis briggsae] 3.0E-72
Protein Domains
Database ID Description E-Value
Pfam IPR003131 EMBL-EBI K+ channel tetramerisation 4.8E-6
Sequence
Target Sequence 
>C. ELEGANS:187666_AT
acgagctgtcgttcaactgtgaggacgcatggcttaacctttttgttggtggagaaatgt
atccagttcaagtaaaaactcttatgaatccaacaacctgtggatcttattttcgggatg
tggttaaagtatctgatgcagcaataaaggttcgaggagttcaatgggatactgcaccaa
atcatatcaagtttcgagtcgatattgatagagatggagttctttttcgacatgtgctcc
aatatctcagaaatggaaaacttacaagcctccccgatgatattttcactcttgagtccc
tagtcgccgaggcagagtttttcggtctcgaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACGAGCTGTCGTTCAACTGTGAGGA 540 93 20 Antisense
GGACGCATGGCTTAACCTTTTTGTT 333 523 42 Antisense
AACTCTTATGAATCCAACAACCTGT 563 139 96 Antisense
ACAACCTGTGGATCTTATTTTCGGG 455 103 112 Antisense
GAGGAGTTCAATGGGATACTGCACC 134 403 173 Antisense
GATGGAGTTCTTTTTCGACATGTGC 641 405 232 Antisense
TCGACATGTGCTCCAATATCTCAGA 338 601 246 Antisense
CCTCCCCGATGATATTTTCACTCTT 591 267 288 Antisense
TATTTTCACTCTTGAGTCCCTAGTC 213 661 300 Antisense
TCCCTAGTCGCCGAGGCAGAGTTTT 387 597 316 Antisense
GAGGCAGAGTTTTTCGGTCTCGAAA 13 405 328 Antisense
Sequence Source Affymetrix Proprietary Database