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[187662_at]

Description F32H5.3 /REP_DB=WormBase Gene ID /WP=CE17751 /CHR=5 /FEA=Sanger Annotation /DEF=(HINXTON) TR:O62210 protein_id:CAB04252.1
Cluster 4grp_high_8_8 > 40      6grp_total > 62      4grp_total > 55      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 512
1 2 3 4 range
A 625.4
(3)
P 		235.3
(-2.8)
P 		267.7
(-2.3)
P 		222.7
(-3)
A 		403
B 624.3
(3)
P 		175.4
(-3)
P 		277.8
(-1.6)
P 		209.8
(-2.5)
P 		449
C 687.5
(3)
P 		177.7
(-3)
A 		358.6
(-0.9)
P 		213
(-2.6)
P 		510
avr 645.7
(3) 		196.1
(-3) 		301.4
(-1.6) 		215.2
(-2.7) 		450

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 766
1 2 3 4 5 6 range
A 167.5
(-2.2)
P 		269.5
(-1.2)
P 		700.2
(3)
P 		517.8
(1.2)
P 		356.3
(-0.3)
P 		81.2
(-3)
A 		619
B 190.1
(-1.8)
P 		356.2
(-0.5)
P 		808.2
(3)
P 		599
(1.4)
P 		408.8
(-0.1)
P 		42
(-3)
A 		766
C 188.7
(-1.9)
P 		387.1
(0.3)
P 		588
(2.6)
P 		625.9
(3)
P 		298.1
(-0.7)
P 		87.9
(-3)
A 		538
avr 182.1
(-1.9) 		337.6
(-0.4) 		698.8
(3) 		580.9
(1.9) 		354.4
(-0.3) 		70.4
(-3) 		628
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 195
1 2 3 4 range
A 227.7
(3)
P 		51.1
(-2.4)
P 		70
(-1.8)
P 		32.6
(-3)
A 		195
B 199.1
(3)
P 		43.3
(-3)
P 		64.4
(-2.2)
P 		44.7
(-2.9)
P 		156
C 227.6
(3)
P 		51.1
(-2.5)
A 		125
(-0.2)
P 		35.9
(-3)
P 		192
avr 218.1
(3) 		48.5
(-2.6) 		86.5
(-1.4) 		37.7
(-3) 		180

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 181
1 2 3 4 5 6 range
A 30.9
(-2.5)
P 		95.9
(0.4)
P 		152.5
(3)
P 		131.3
(2)
P 		107.5
(1)
P 		19.8
(-3)
A 		133
B 66.1
(-1.3)
P 		118.6
(0.4)
P 		196.3
(3)
P 		158.7
(1.8)
P 		166.1
(2)
P 		15.7
(-3)
A 		181
C 69.9
(-1)
P 		118.1
(1.4)
P 		149.7
(3)
P 		125.9
(1.8)
P 		96.5
(0.3)
P 		29.4
(-3)
A 		120
avr 55.6
(-1.6) 		110.9
(0.7) 		166.2
(3) 		138.6
(1.9) 		123.4
(1.2) 		21.6
(-3) 		145
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187662_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13333
Sequence Type Exemplar sequence
Representative Public ID F32H5.3 NCBI
Target Description F32H5.3 /REP_DB=WormBase Gene ID /WP=CE17751 /CHR=5 /FEA=Sanger Annotation /DEF=(HINXTON) TR:O62210 protein_id:CAB04252.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073910(11), NM_073911(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073910 NCBI Caenorhabditis elegans F32H5.3a (F32H5.3) mRNA, complete cds. 11/11 None
NM_073911 NCBI Caenorhabditis elegans F32H5.3b (F32H5.3) mRNA, complete cds. 10/11 None
SNAP00000043409 ENSEMBL cdna:SNAP chromosome:CEL140:V:13362014:13362356:1 10/11 None
GENEFINDER00000043413 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:13362014:13362356:1 10/11 None
F32H5.3a ENSEMBL cdna:known chromosome:CEL140:V:13361926:13362546:1 gene:F32H5.3 11/11 None
F32H5.3b ENSEMBL cdna:known chromosome:CEL140:V:13361991:13362619:1 gene:F32H5.3 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:13362013-13362356(+) 100.0 100.0
Public Domain and Genome References
Ensembl F32H5.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04252 Hypothetical protein F32H5.3a [Caenorhabditis elegans] ref|NP_506311.1| F32H5.3a [Caenorhabditis elegans] 2.0E-49
blast CAC42293 Hypothetical protein F32H5.3b [Caenorhabditis elegans] ref|NP_506312.1| F32H5.3b [Caenorhabditis elegans] 2.0E-47
blast CAC42293 Hypothetical protein F32H5.3b [Caenorhabditis elegans] ref|NP_506312.1| F32H5.3b [Caenorhabditis elegans] 7.0E-46
blast CAB04252 Hypothetical protein F32H5.3a [Caenorhabditis elegans] ref|NP_506311.1| F32H5.3a [Caenorhabditis elegans] 7.0E-46
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_506311.1 1 2-24
NP_506312.1 1 2-24
F32H5.3a 1 2-24
F32H5.3b 1 2-24
SNAP00000043409 1 2-24
GENEFINDER00000043413 1 2-24
Sequence
Target Sequence 
>C. ELEGANS:187662_AT
ggtctcaattacctacattttgctcatttcaactgtaactgtgctcacagtatcagcagc
tccaattgtgacggaacaaaaagcttttccagatatcgctgaattctcaaattccaacaa
ggacgcaaacgtctttcttccattccgtgaactccctgaacgtcttatcgatgatttcaa
ttcaaaggaactcgatgagggacgtgatcttccatcagaatctgacattttctacttctt
cggaaagacgccggttctgacggaagt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGTCTCAATTACCTACATTTTGCTC 253 503 15 Antisense
GCTCATTTCAACTGTAACTGTGCTC 471 301 36 Antisense
TGTGCTCACAGTATCAGCAGCTCCA 392 557 54 Antisense
GCAGCTCCAATTGTGACGGAACAAA 533 311 70 Antisense
AAAGCTTTTCCAGATATCGCTGAAT 139 125 94 Antisense
ATCGCTGAATTCTCAAATTCCAACA 545 37 109 Antisense
AGGACGCAAACGTCTTTCTTCCATT 76 55 134 Antisense
CGTGAACTCCCTGAACGTCTTATCG 219 67 160 Antisense
ATGAGGGACGTGATCTTCCATCAGA 76 45 209 Antisense
GACATTTTCTACTTCTTCGGAAAGA 220 367 238 Antisense
GAAAGACGCCGGTTCTGACGGAAGT 692 357 257 Antisense
Sequence Source Affymetrix Proprietary Database