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[187648_s_at]

Description C04A2.7A /REP_DB=WormBase Gene ID /WP=CE01772 /TR=SW:Q09446 /GB=AAC46736.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 52      6grp_high1_10_10 > 35      4grp_highest_8_8 > 51      4grp_high_8_8 > 32      6grp_highest_nonorm > 10      6grp_total > 26      4grp_total > 47      6grp_highest_sub > 01_02      6grp_cgc > 14      6grp_high1_sub > 02      6grp_cgc_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 613
1 2 3 4 range
A 1288.3
(3)
P 		860
(-1.4)
P 		704.9
(-3)
P 		776.7
(-2.3)
P 		583
B 1265.2
(3)
P 		699.1
(-3)
P 		714.1
(-2.8)
P 		899.9
(-0.9)
P 		566
C 1228.8
(3)
P 		675.8
(-3)
P 		703.5
(-2.7)
P 		709.3
(-2.6)
P 		553
avr 1260.8
(3) 		745
(-2.6) 		707.5
(-3) 		795.3
(-2) 		553

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 790
1 2 3 4 5 6 range
A 1565.1
(2.7)
P 		1601.5
(3)
P 		848.2
(-3)
P 		1127.4
(-0.8)
P 		1112.6
(-0.9)
P 		1300.3
(0.6)
P 		753
B 1638.2
(3)
P 		1606.2
(2.7)
P 		1163.2
(-0.9)
P 		1200.4
(-0.6)
P 		914.8
(-3)
P 		1216.3
(-0.5)
P 		723
C 1434.4
(1.5)
P 		1614.3
(3)
P 		1330.7
(0.6)
P 		1097.5
(-1.4)
P 		916
(-3)
P 		1112.4
(-1.3)
P 		698
avr 1545.9
(2.4) 		1607.3
(3) 		1114
(-1.7) 		1141.8
(-1.5) 		981.1
(-3) 		1209.7
(-0.8) 		626
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 355
1 2 3 4 range
A 469.1
(3)
P 		186.7
(-1.8)
P 		184.3
(-1.8)
P 		113.7
(-3)
P 		355
B 403.5
(3)
P 		172.7
(-2.8)
P 		165.5
(-3)
P 		191.8
(-2.3)
P 		238
C 406.8
(3)
P 		194.3
(-1.4)
P 		245.2
(-0.4)
P 		119.6
(-3)
P 		287
avr 426.5
(3) 		184.6
(-2.1) 		198.3
(-1.8) 		141.7
(-3) 		285

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 385
1 2 3 4 5 6 range
A 288.5
(-1.4)
P 		569.6
(3)
P 		184.7
(-3)
P 		285.9
(-1.4)
P 		335.7
(-0.6)
P 		316.6
(-0.9)
P 		385
B 570
(3)
P 		534.6
(2.3)
P 		282.5
(-3)
P 		317.9
(-2.3)
P 		371.7
(-1.1)
P 		453.6
(0.6)
P 		288
C 531.7
(3)
P 		492.6
(2.2)
P 		338.7
(-0.7)
P 		220.7
(-3)
P 		296.4
(-1.5)
P 		372.4
(-0.1)
P 		311
avr 463.4
(1.4) 		532.3
(3) 		268.6
(-3) 		274.8
(-2.9) 		334.6
(-1.5) 		380.9
(-0.4) 		264
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 187648_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4650
Sequence Type Exemplar sequence
Representative Public ID C04A2.7A NCBI
Target Description C04A2.7A /REP_DB=WormBase Gene ID /WP=CE01772 /TR=SW:Q09446 /GB=AAC46736.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171014(11), NM_062999(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171014 NCBI Caenorhabditis elegans DNaJ domain (prokaryotic heat shock protein) family member (dnj-5) (dnj-5) mRNA, complete cds. 11/11 A 
NM_062999 NCBI Caenorhabditis elegans DNaJ domain (prokaryotic heat shock protein) family member (dnj-5) (dnj-5) mRNA, complete cds. 11/11 A 
SNAP00000035666 ENSEMBL cdna:SNAP chromosome:CEL140:II:6853814:6854247:-1 10/11 None
C04A2.7a ENSEMBL cdna:known chromosome:CEL140:II:6853399:6858202:-1 gene:C04A2.7 11/11 A 
C04A2.7c.1 ENSEMBL cdna:known chromosome:CEL140:II:6853399:6858218:-1 gene:C04A2.7 11/11 None
C04A2.7c.2 ENSEMBL cdna:known chromosome:CEL140:II:6853404:6858225:-1 gene:C04A2.7 11/11 None
C04A2.7b.1 ENSEMBL cdna:known chromosome:CEL140:II:6853424:6858218:-1 gene:C04A2.7 10/11 None
C04A2.7b.2 ENSEMBL cdna:known chromosome:CEL140:II:6853424:6858228:-1 gene:C04A2.7 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_171015 5/11 Cross Hyb Matching Probes A 
C04A2.7d 5/11 Cross Hyb Matching Probes A 
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
174122 4 2 NM_062999 187648_s_at (11)
NM_171014 187648_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:6853809-6858146(-) 100.0 100.0
Public Domain and Genome References
Ensembl C04A2.7
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAL27227 Dnaj domain (prokaryotic heat shock protein) protein 5, isoform c [Caenorhabditis elegans] ref|NP_741015.1| DNaJ domain (prokaryotic heat shock protein) family member (dnj-5) [Caenorhabditis elegans] 0.0
blast AAL27225 Dnaj domain (prokaryotic heat shock protein) protein 5, isoform a [Caenorhabditis elegans] ref|NP_495400.2| DNaJ domain (prokaryotic heat shock protein) family member (dnj-5) [Caenorhabditis elegans] sp|Q09446|DNJ5_CAEEL DnaJ homolog dnj-5 (DnaJ domain protein 5) 0.0
blast AAM81128 Dnaj domain (prokaryotic heat shock protein) protein 5, isoform d [Caenorhabditis elegans] ref|NP_741016.1| DNaJ domain (prokaryotic heat shock protein) family member (dnj-5) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
scop a.2.3.Chaperone J-domain All alpha proteins; Long alpha-hairpin; Chaperone J-domain; Chaperone J-domain 2.00000002337219E-7
Pfam IPR001623 EMBL-EBI Heat shock protein DnaJ, N-terminal 1.5E-12
Pfam IPR001623 EMBL-EBI Heat shock protein DnaJ, N-terminal 1.5E-12
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_741015.1 2 543-565,586-605
NP_495400.2 2 544-566,587-606
C04A2.7a 2 544-566,587-606
C04A2.7c.1 2 543-565,586-605
C04A2.7c.2 2 543-565,586-605
C04A2.7b.1 2 544-566,587-606
C04A2.7b.2 2 544-566,587-606
Sequence
Target Sequence 
>C. ELEGANS:187648_S_AT
gctacttgcaagagccaacgtgcgatgctcaaaaacatgcgagcacacactcacaatgtc
cagtatcggttgttatcgccaatgaactccgactttgatgcccaatctcaatattacaac
tcgtcatatccatcctcttcaacagacgctcgttttttgagcgatcttcaagaagagtac
tcgactttgctccgccagcgtagtcttgctcaaatttctttcaaaaactgtgaaccacgt
gaggaggatcggagtcgtcgtgca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTACTTGCAAGAGCCAACGTGCGA 74 303 2167 Antisense
AGCCAACGTGCGATGCTCAAAAACA 549 83 2179 Antisense
AAACATGCGAGCACACACTCACAAT 370 127 2199 Antisense
GCACACACTCACAATGTCCAGTATC 597 317 2209 Antisense
GTCCAGTATCGGTTGTTATCGCCAA 186 477 2224 Antisense
TATCGCCAATGAACTCCGACTTTGA 163 653 2240 Antisense
CCGACTTTGATGCCCAATCTCAATA 678 263 2255 Antisense
TATTACAACTCGTCATATCCATCCT 631 663 2278 Antisense
CCTCTTCAACAGACGCTCGTTTTTT 11 267 2300 Antisense
CTCGTTTTTTGAGCGATCTTCAAGA 680 229 2315 Antisense
TGAGGAGGATCGGAGTCGTCGTGCA 315 407 2406 Antisense
Sequence Source Affymetrix Proprietary Database