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[187643_at]

Description F23F12.1 /REP_DB=WormBase Gene ID /WP=CE01247 /TR=SW:P46497 /GB=AAA20603.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 30      4grp_total > 60      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 154
1 2 3 4 range
A 138.3
(1.7)
P 		97.9
(-0.5)
P 		163.6
(3)
P 		51
(-3)
A 		113
B 95.4
(3)
P 		92.4
(2.8)
A 		93.8
(2.9)
P 		11.3
(-3)
A 		84
C 136.3
(3)
P 		135.3
(3)
A 		100.6
(1.3)
P 		9.4
(-3)
A 		127
avr 123.3
(3) 		108.5
(2.1) 		119.3
(2.8) 		23.9
(-3) 		99

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 98
1 2 3 4 5 6 range
A 91.5
(0.2)
A 		116.3
(1.8)
P 		102
(0.9)
A 		136.4
(3)
P 		72
(-1)
A 		38.8
(-3)
A 		98
B 39.5
(-3)
A 		86
(1.1)
A 		64
(-0.8)
A 		107.1
(3)
P 		54.2
(-1.7)
A 		54.3
(-1.7)
A 		68
C 59.8
(-2.8)
A 		105.7
(2)
P 		115
(3)
A 		60.6
(-2.7)
P 		70.2
(-1.7)
A 		58.1
(-3)
P 		57
avr 63.6
(-1.5) 		102.7
(3) 		93.7
(2) 		101.4
(2.9) 		65.5
(-1.3) 		50.4
(-3) 		52
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 49
1 2 3 4 range
A 50.3
(3)
P 		21.3
(-1.1)
P 		42.8
(1.9)
P 		7.5
(-3)
A 		43
B 30.4
(3)
P 		22.8
(1.4)
A 		21.7
(1.1)
P 		2.4
(-3)
A 		28
C 45.1
(3)
P 		38.9
(2.1)
A 		35.1
(1.6)
P 		1.6
(-3)
A 		44
avr 41.9
(3) 		27.7
(0.8) 		33.2
(1.6) 		3.8
(-3) 		38

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 32
1 2 3 4 5 6 range
A 16.9
(-1.6)
A 		41.4
(3)
P 		22.2
(-0.6)
A 		34.6
(1.7)
P 		21.7
(-0.7)
A 		9.4
(-3)
A 		32
B 13.7
(-3)
A 		28.6
(3)
A 		15.5
(-2.3)
A 		28.4
(2.9)
P 		22
(0.3)
A 		20.2
(-0.4)
A 		15
C 22.2
(-0)
A 		32.3
(3)
P 		29.3
(2.1)
A 		12.2
(-3)
P 		22.7
(0.1)
A 		19.4
(-0.9)
P 		20
avr 17.6
(-2.6) 		34.1
(3) 		22.3
(-1) 		25.1
(-0) 		22.1
(-1) 		16.3
(-3) 		18
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187643_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7392
Sequence Type Exemplar sequence
Representative Public ID F23F12.1 NCBI
Target Description F23F12.1 /REP_DB=WormBase Gene ID /WP=CE01247 /TR=SW:P46497 /GB=AAA20603.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066032(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066032 NCBI Caenorhabditis elegans F23F12.3 (F23F12.3) mRNA, complete cds. 11/11 None
GENEFINDER00000039917 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:6505191:6508174:1 10/11 None
SNAP00000039908 ENSEMBL cdna:SNAP chromosome:CEL140:III:6505949:6507560:1 11/11 None
F23F12.3 ENSEMBL cdna:known chromosome:CEL140:III:6500394:6507652:1 gene:F23F12.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:6505847-6509248(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F23F12.3
Entrez Gene 175926 Entrez gene
SwissProt P46499 EMBL-EBI
WormBase CE37224 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6810 transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5215 transporter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_498433 F23F12.3 [Caenorhabditis elegans] gb|AAA20605.3| Hypothetical protein F23F12.3 [Caenorhabditis elegans] sp|P46499|YLX3_CAEEL Hypothetical protein F23F12.3 in chromosome III 0.0
blast AAY86205 Hypothetical protein F23F12.13 [Caenorhabditis elegans] 0.0
blast NP_498433 F23F12.3 [Caenorhabditis elegans] gb|AAA20605.3| Hypothetical protein F23F12.3 [Caenorhabditis elegans] sp|P46499|YLX3_CAEEL Hypothetical protein F23F12.3 in chromosome III 1.0E-173
blast CAE73649 Hypothetical protein CBG21150 [Caenorhabditis briggsae] 1.0E-144
blast AAY86205 Hypothetical protein F23F12.13 [Caenorhabditis elegans] 1.0E-124
blast CAE73649 Hypothetical protein CBG21150 [Caenorhabditis briggsae] 1.0E-124
Protein Domains
Database ID Description E-Value
Pfam IPR005828 EMBL-EBI General substrate transporter 7.6E-7
Pfam IPR005828 EMBL-EBI General substrate transporter 7.6E-7
Pfam IPR005828 EMBL-EBI General substrate transporter 9.5E-9
Pfam IPR005828 EMBL-EBI General substrate transporter 7.6E-7
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_498433.2 12 13-35,96-118,125-147,151-173,200-222,226-243,298-320,340-362,369-389,404-426,438-460,465-487
F23F12.3 12 13-35,96-118,125-147,151-173,200-222,226-243,298-320,340-362,369-389,404-426,438-460,465-487
GENEFINDER00000039917 9 50-67,74-96,123-145,221-243,263-285,292-312,327-349,361-383,388-410
SNAP00000039908 7 57-79,134-156,176-198,205-225,240-262,274-296,301-323
Sequence
Target Sequence 
>C. ELEGANS:187643_AT
gcgggaaatttctatctgagtcaattcgttcaagcagctgcaattgccctatctaaactt
tccattttcctcctcgatctcttcattccatcattcaaccgccaacgccttcaccaagtt
ccccaaataataatgattgcttgttacactacaatcatggcattgatgatttctccagac
tcagactgttctagccaagggtctaggaatttagcgagcattggtacatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCGGGAAATTTCTATCTGAGTCAAT 499 291 343 Antisense
GAGTCAATTCGTTCAAGCAGCTGCA 599 399 360 Antisense
TCAAGCAGCTGCAATTGCCCTATCT 179 631 372 Antisense
TGCCCTATCTAAACTTTCCATTTTC 322 585 387 Antisense
ACCAAGTTCCCCAAATAATAATGAT 705 83 455 Antisense
AATAATGATTGCTTGTTACACTACA 539 171 471 Antisense
GTTACACTACAATCATGGCATTGAT 547 445 485 Antisense
GGCATTGATGATTTCTCCAGACTCA 7 537 501 Antisense
TCTCCAGACTCAGACTGTTCTAGCC 657 607 514 Antisense
GACTGTTCTAGCCAAGGGTCTAGGA 639 375 526 Antisense
GGAATTTAGCGAGCATTGGTACATG 119 533 548 Antisense
Sequence Source Affymetrix Proprietary Database