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[187618_at]

Description Y46G5A.31 /REP_DB=WormBase Gene ID /WP=CE24302 /TR=SW:Q9U2D9 /GB=CAB60373.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 13      6grp_high1_10_10 > 30      4grp_high_8_8 > 50      6grp_highest_nonorm > 74      6grp_total > 44      4grp_total > 61      6grp_cgc > 80      6grp_high1_sub > 03      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 818
1 2 3 4 range
A 1021.6
(2.9)
P 		1031.7
(3)
P 		896
(2)
P 		226.3
(-3)
A 		805
B 1044.4
(3)
P 		690.8
(0)
P 		809.3
(1)
P 		336.3
(-3)
P 		708
C 1015.6
(3)
P 		944.4
(2.3)
P 		675.8
(-0.2)
P 		373.7
(-3)
M 		642
avr 1027.2
(3) 		889
(1.8) 		793.7
(1) 		312.1
(-3) 		715

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 916
1 2 3 4 5 6 range
A 642.3
(-1.2)
P 		936.3
(3)
P 		580.6
(-2.1)
P 		758.1
(0.5)
P 		516.7
(-3)
P 		836.1
(1.6)
P 		420
B 501.4
(0.7)
P 		494.7
(0.2)
P 		506.7
(1)
P 		442.8
(-3)
P 		538.8
(3)
P 		501.2
(0.7)
P 		96
C 1206.1
(1.9)
P 		530.1
(-2.8)
P 		1358.6
(3)
P 		494.7
(-3)
P 		718.4
(-1.4)
P 		579
(-2.4)
P 		864
avr 783.3
(2.2) 		653.7
(-0.9) 		815.3
(3) 		565.2
(-3) 		591.3
(-2.4) 		638.8
(-1.2) 		250
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 339
1 2 3 4 range
A 372
(3)
P 		223.9
(0.4)
P 		234.2
(0.6)
P 		33.1
(-3)
A 		339
B 333
(3)
P 		170.6
(-0.7)
P 		187.5
(-0.3)
P 		71.7
(-3)
P 		261
C 336.2
(3)
P 		271.5
(1.6)
P 		235.5
(0.8)
P 		63
(-3)
M 		273
avr 347.1
(3) 		222
(0.4) 		219.1
(0.4) 		55.9
(-3) 		291

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 348
1 2 3 4 5 6 range
A 118.4
(-3)
P 		333
(3)
P 		126.4
(-2.8)
P 		192.3
(-0.9)
P 		155.9
(-2)
P 		203.6
(-0.6)
P 		215
B 174.5
(0.4)
P 		164.7
(-0.2)
P 		123
(-2.7)
P 		117.3
(-3)
P 		218.9
(3)
P 		186.9
(1.1)
P 		102
C 447
(3)
P 		161.8
(-1.9)
P 		345.8
(1.3)
P 		99.5
(-3)
P 		232.5
(-0.7)
P 		193.8
(-1.4)
P 		348
avr 246.6
(3) 		219.8
(1.5) 		198.4
(0.4) 		136.4
(-3) 		202.4
(0.6) 		194.8
(0.2) 		110
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187618_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3433
Sequence Type Exemplar sequence
Representative Public ID Y46G5A.31 NCBI
Target Description Y46G5A.31 /REP_DB=WormBase Gene ID /WP=CE24302 /TR=SW:Q9U2D9 /GB=CAB60373.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064335(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064335 NCBI Caenorhabditis elegans Glycogen SYnthase family member (gsy-1) (gsy-1) mRNA, complete cds. 11/11 None
SNAP00000039015 ENSEMBL cdna:SNAP chromosome:CEL140:II:12866745:12877988:-1 11/11 None
GENEFINDER00000039017 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:12866745:12877377:-1 11/11 None
Y46G5A.31 ENSEMBL cdna:known chromosome:CEL140:II:12866359:12878001:-1 gene:Y46G5A.31 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:12866741-12877985(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol gsy-1
Ensembl Y46G5A.31
Entrez Gene 174924 Entrez gene
SwissProt Q9U2D9 EMBL-EBI
WormBase CE24302 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1630044_S_AT dm
DROSGENOME1:141226_AT dm
HG-U133A:201673_S_AT glycogen synthase 1 (muscle) hs
HG-U133_PLUS_2:201673_S_AT glycogen synthase 1 (muscle) hs
HG-FOCUS:201673_S_AT glycogen synthase 1 (muscle) hs
HG-U133A_2:201673_S_AT glycogen synthase 1 (muscle) hs
HUGENEFL:J04501_AT glycogen synthase 1 (muscle) hs
U133_X3P:G4504232_3P_A_AT glycogen synthase 1 (muscle) hs
HG-U95AV2:37299_AT glycogen synthase 1 (muscle) hs
MOE430A:1450196_S_AT glycogen synthase 1, muscle mm
MOUSE430_2:1450196_S_AT glycogen synthase 1, muscle mm
MOUSE430A_2:1450196_S_AT glycogen synthase 1, muscle mm
MOE430A:1450196_S_AT glycogen synthase 3, brain mm
MOUSE430_2:1450196_S_AT glycogen synthase 3, brain mm
MOUSE430A_2:1450196_S_AT glycogen synthase 3, brain mm
MU11KSUBB:MSA.2317.0_F_AT glycogen synthase 3, brain mm
MG-U74AV2:98496_AT glycogen synthase 3, brain mm
MG-U74CV2:169225_AT glycogen synthase 3, brain mm
MOE430A:1416737_AT glycogen synthase 3, brain mm
MOUSE430A_2:1416737_AT glycogen synthase 3, brain mm
MOUSE430_2:1416737_AT glycogen synthase 3, brain mm
MU19KSUBC:TC36244_S_AT Glycogen synthase 1, muscle, mRNA (cDNA clone MGC:25254 IMAGE:3663506) mm
YEAST_2:1779105_AT Glycogen synthase, similar to Gsy1p; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
5978 glycogen biosynthesis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4373 glycogen (starch) synthase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB60373 Hypothetical protein Y46G5A.31 [Caenorhabditis elegans] ref|NP_496736.1| Glycogen SYnthase family member (gsy-1) [Caenorhabditis elegans] sp|Q9U2D9|GYS_CAEEL Probable glycogen [starch] synthase 0.0
blast CAE71482 Hypothetical protein CBG18401 [Caenorhabditis briggsae] 0.0
blast AAK28335 glycogen synthase [Steinernema feltiae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR008631 EMBL-EBI Glycogen synthase 1.0E-126
Pfam IPR008631 EMBL-EBI Glycogen synthase 1.0E-126
Pfam IPR008631 EMBL-EBI Glycogen synthase 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:187618_AT
aaagttgtcttccatccagaattcctgtcatctgtctctccactaattggcctggactac
gaggatttcgtgcgtggttgccatttgggagtattcccatcttattacgaaccatgggga
tatacgccagccgagtgtaccgtaatgggaatcccgtcggtctcgacgaatctctctggt
ttcggatgcttcatgcaggaacacgtcgaggatcacgagcaaaaaggaatctatgtgatt
gatcggagacataaggcggcggaagagtctgtgcaagagttggcacaggttatgtacgat
ttctgtggacaatctcgtcgtcaacgcatcattcttcgtaattccaatgagggactctcc
gcgctgcttgattggcagaatcttggagttttctatcgtgactgccgccgcctcgccctg
gaacgtcttcatcccgacgtggacaagatcatgcgtgacaatgaaggaaaagtgccgtca
gctgcca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAGTTGTCTTCCATCCAGAATTCC 169 123 1495 Antisense
CCAGAATTCCTGTCATCTGTCTCTC 62 273 1510 Antisense
GTCTCTCCACTAATTGGCCTGGACT 7 471 1528 Antisense
TATACGCCAGCCGAGTGTACCGTAA 109 657 1615 Antisense
GGAATCCCGTCGGTCTCGACGAATC 210 529 1642 Antisense
GACGAATCTCTCTGGTTTCGGATGC 306 379 1659 Antisense
CTGTGGACAATCTCGTCGTCAACGC 187 239 1797 Antisense
GTCGTCAACGCATCATTCTTCGTAA 492 473 1811 Antisense
TCTCCGCGCTGCTTGATTGGCAGAA 25 611 1850 Antisense
GTCTTCATCCCGACGTGGACAAGAT 587 467 1919 Antisense
GAAGGAAAAGTGCCGTCAGCTGCCA 473 333 1957 Antisense
Sequence Source Affymetrix Proprietary Database