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[187611_s_at]

Description F53F10.3 /REP_DB=WormBase Gene ID /WP=CE28447 /TR=SW:O01578 /GB=AAB52430.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 03      6grp_high1_10_10 > 32      4grp_highest_8_8 > 33      4grp_high_8_8 > 50      6grp_highest_nonorm > 23      6grp_total > 32      4grp_total > 61      6grp_highest_sub > De      6grp_high1_sub > 02_03      6grp_total_sub > Hat      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1124
1 2 3 4 range
A 2095.2
(3)
P 		1487.1
(-0.8)
P 		1736.7
(0.7)
P 		1144.5
(-3)
P 		951
B 1815
(3)
P 		1650.4
(1.8)
P 		1507.5
(0.8)
P 		971.3
(-3)
P 		844
C 1695.7
(3)
P 		1694.3
(3)
P 		1577.7
(2)
P 		995
(-3)
P 		701
avr 1868.6
(3) 		1610.6
(1.1) 		1607.3
(1.1) 		1036.9
(-3) 		832

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1324
1 2 3 4 5 6 range
A 2111.7
(0.5)
P 		2413.9
(2.8)
P 		2439.2
(3)
P 		2033.5
(-0.1)
P 		1911.3
(-1.1)
P 		1665.3
(-3)
P 		774
B 2062.5
(1.6)
P 		2260.1
(2.9)
P 		2281.9
(3)
P 		1859.2
(0.2)
P 		2109.8
(1.9)
P 		1372.5
(-3)
P 		909
C 2236.6
(1.4)
P 		2339.7
(1.9)
P 		2582.9
(3)
P 		1964.3
(0.2)
P 		2225.7
(1.4)
P 		1259.1
(-3)
P 		1324
avr 2136.9
(1.2) 		2337.9
(2.4) 		2434.7
(3) 		1952.3
(0.1) 		2082.3
(0.9) 		1432.3
(-3) 		1002
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 595
1 2 3 4 range
A 762.9
(3)
P 		322.8
(-1.4)
P 		454
(-0.1)
P 		167.5
(-3)
P 		595
B 578.8
(3)
P 		407.6
(0.2)
P 		349.3
(-0.7)
P 		207
(-3)
P 		372
C 561.3
(3)
P 		487.1
(1.9)
P 		549.9
(2.8)
P 		167.8
(-3)
P 		394
avr 634.3
(3) 		405.8
(-0) 		451.1
(0.6) 		180.8
(-3) 		454

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 469
1 2 3 4 5 6 range
A 389.3
(-3)
P 		858.5
(3)
P 		531.2
(-1.2)
P 		515.7
(-1.4)
P 		576.8
(-0.6)
P 		405.4
(-2.8)
P 		469
B 717.7
(0.7)
P 		752.3
(1.3)
P 		554.1
(-2)
P 		492.4
(-3)
P 		857.2
(3)
P 		511.8
(-2.7)
P 		365
C 829
(3)
P 		713.9
(1.4)
P 		657.4
(0.6)
P 		395
(-3)
P 		720.2
(1.5)
P 		421.5
(-2.6)
P 		434
avr 645.3
(0.6) 		774.9
(3) 		580.9
(-0.5) 		467.7
(-2.6) 		718.1
(2) 		446.2
(-3) 		329
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 187611_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.331
Sequence Type Exemplar sequence
Representative Public ID F53F10.3 NCBI
Target Description F53F10.3 /REP_DB=WormBase Gene ID /WP=CE28447 /TR=SW:O01578 /GB=AAB52430.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058833(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058833 NCBI Caenorhabditis elegans F53F10.3 (F53F10.3) mRNA, complete cds. 11/11 None
GENEFINDER00000021863 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:3826692:3828039:1 11/11 None
SNAP00000021855 ENSEMBL cdna:SNAP chromosome:CEL140:I:3827239:3828039:1 11/11 None
F53F10.3.2 ENSEMBL cdna:known chromosome:CEL140:I:3827219:3828145:1 gene:F53F10.3 11/11 None
F53F10.3.1 ENSEMBL cdna:known chromosome:CEL140:I:3827221:3828133:1 gene:F53F10.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:3827238-3828990(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F53F10.3
Entrez Gene 171957 Entrez gene
SwissProt O01578 EMBL-EBI
WormBase CE28447 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:245571_AT expressed protein at
CANINE:1590738_AT similar to Brain protein 44 cfa
CANINE_2:CFA.13282.1.S1_S_AT similar to Brain protein 44 cfa
CANINE_2:CFA.13282.2.A1_A_AT similar to Brain protein 44 cfa
DROSOPHILA_2:1638009_S_AT dm
DROSGENOME1:149569_AT dm
HG-U95AV2:37000_AT brain protein 44 hs
HU35KSUBA:AA428006_AT brain protein 44 hs
HU35KSUBC:RC_AA281132_AT brain protein 44 hs
HG-U133A:202427_S_AT brain protein 44 hs
HG-U133_PLUS_2:202427_S_AT brain protein 44 hs
HG-U133A_2:202427_S_AT brain protein 44 hs
U133_X3P:G7661601_3P_A_AT brain protein 44 hs
HU35KSUBB:RC_R10684_AT Brain protein 44 hs
U133_X3P:HS.5794.0.A1_3P_AT Brain protein 44 hs
HG-U133_PLUS_2:227669_AT Brain protein 44 hs
HG-U133B:227669_AT Brain protein 44 hs
HG-U95B:46134_AT Brain protein 44 hs
MG-U74AV2:95058_F_AT brain protein 44 mm
MG-U74BV2:165362_F_AT brain protein 44 mm
MG-U74CV2:168915_R_AT brain protein 44 mm
MOE430A:1423833_A_AT brain protein 44 mm
MOUSE430A_2:1423833_A_AT brain protein 44 mm
MOUSE430_2:1423833_A_AT brain protein 44 mm
MOE430B:1430071_AT brain protein 44 mm
MOUSE430_2:1430071_AT brain protein 44 mm
MU19KSUBC:TC40770_AT Brain protein 44 (Brp44), mRNA mm
MU11KSUBB:W40971_S_AT Brain protein 44 (Brp44), mRNA mm
Protein Similarities
Method ID Description E-Value
blast AAB52430 Hypothetical protein F53F10.3 [Caenorhabditis elegans] ref|NP_491234.1| F53F10.3 [Caenorhabditis elegans] sp|O01578|YXX3_CAEEL Hypothetical UPF0041 protein F53F10.3 in chromosome I 7.0E-77
blast AAB52430 Hypothetical protein F53F10.3 [Caenorhabditis elegans] ref|NP_491234.1| F53F10.3 [Caenorhabditis elegans] sp|O01578|YXX3_CAEEL Hypothetical UPF0041 protein F53F10.3 in chromosome I 1.0E-76
blast CAE66673 Hypothetical protein CBG12012 [Caenorhabditis briggsae] 4.0E-74
blast CAE66673 Hypothetical protein CBG12012 [Caenorhabditis briggsae] 8.0E-74
Protein Domains
Database ID Description E-Value
Pfam IPR005336 EMBL-EBI Protein of unknown function UPF0041 3.2E-73
Pfam IPR005336 EMBL-EBI Protein of unknown function UPF0041 3.2E-73
Sequence
Target Sequence 
>C. ELEGANS:187611_S_AT
tcaaatggactcttatcggcgctggactcgctgatttggccagacctgctgataagcttt
ctctttatcagaactctgctctcttcgccactggagccatctggacgagatattgccttg
tcatcaccccaatcaactactatttgagtagtgtgaactttttcgtcatgtgcaccgggc
tcgcccagctctgtagaattgcac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCAAATGGACTCTTATCGGCGCTGG 205 629 152 Antisense
CTGATTTGGCCAGACCTGCTGATAA 560 237 182 Antisense
AGACCTGCTGATAAGCTTTCTCTTT 94 71 193 Antisense
AGCTTTCTCTTTATCAGAACTCTGC 517 79 206 Antisense
TTTATCAGAACTCTGCTCTCTTCGC 505 673 215 Antisense
CCACTGGAGCCATCTGGACGAGATA 432 275 239 Antisense
GACGAGATATTGCCTTGTCATCACC 282 379 255 Antisense
CATCACCCCAATCAACTACTATTTG 314 213 273 Antisense
GAGTAGTGTGAACTTTTTCGTCATG 293 399 297 Antisense
GTGAACTTTTTCGTCATGTGCACCG 481 481 304 Antisense
CTCGCCCAGCTCTGTAGAATTGCAC 538 233 331 Antisense
Sequence Source Affymetrix Proprietary Database