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[187609_s_at]

Description F23F12.1 /REP_DB=WormBase Gene ID /WP=CE01247 /TR=SW:P46497 /GB=AAA20603.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 35      6grp_high1_10_10 > 53      4grp_high_8_8 > 58      6grp_highest_nonorm > 80      6grp_total > 30      4grp_total > 61      6grp_highest_sub > 03      6grp_high1_sub > 03      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 244
1 2 3 4 range
A 190.5
(1.9)
P 		188.3
(1.8)
P 		223.7
(3)
P 		46.9
(-3)
A 		177
B 290.5
(3)
P 		199.5
(0.7)
P 		195
(0.6)
P 		49.5
(-3)
M 		241
C 288.1
(3)
P 		151.9
(-0.7)
P 		213.5
(1)
P 		68.4
(-3)
P 		220
avr 256.4
(3) 		179.9
(0.7) 		210.7
(1.6) 		54.9
(-3) 		202

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 205
1 2 3 4 5 6 range
A 151.6
(-2)
P 		242.7
(0.7)
P 		323
(3)
P 		252.3
(0.9)
P 		117.6
(-3)
P 		243.9
(0.7)
P 		205
B 202.7
(0.9)
P 		238.3
(3)
P 		223.2
(2.1)
P 		177
(-0.6)
P 		163.6
(-1.4)
P 		137
(-3)
P 		101
C 136.3
(-3)
P 		273.5
(1.8)
P 		259.3
(1.3)
P 		307.4
(3)
P 		139.3
(-2.9)
P 		176.9
(-1.6)
P 		171
avr 163.5
(-1.9) 		251.5
(2.2) 		268.5
(3) 		245.6
(1.9) 		140.2
(-3) 		185.9
(-0.9) 		128
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 89
1 2 3 4 range
A 69.4
(3)
P 		40.9
(0.3)
P 		58.5
(2)
P 		6.9
(-3)
A 		63
B 92.7
(3)
P 		49.3
(-0.2)
P 		45.2
(-0.5)
P 		10.6
(-3)
M 		82
C 95.4
(3)
P 		43.7
(-0.7)
P 		74.4
(1.5)
P 		11.5
(-3)
P 		84
avr 85.8
(3) 		44.6
(-0.2) 		59.4
(0.9) 		9.7
(-3) 		76

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 58
1 2 3 4 5 6 range
A 28
(-3)
P 		86.3
(3)
P 		70.3
(1.4)
P 		64
(0.7)
P 		35.5
(-2.2)
P 		59.4
(0.2)
P 		58
B 70.5
(1.4)
P 		79.3
(3)
P 		54.2
(-1.6)
P 		46.9
(-3)
P 		66.5
(0.6)
P 		51.1
(-2.2)
P 		32
C 50.5
(-2.2)
P 		83.4
(3)
P 		66
(0.3)
P 		61.8
(-0.4)
P 		45.1
(-3)
P 		59.2
(-0.8)
P 		38
avr 49.7
(-2.9) 		83
(3) 		63.5
(-0.4) 		57.6
(-1.5) 		49
(-3) 		56.6
(-1.7) 		34
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 187609_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7392
Sequence Type Exemplar sequence
Representative Public ID F23F12.1 NCBI
Target Description F23F12.1 /REP_DB=WormBase Gene ID /WP=CE01247 /TR=SW:P46497 /GB=AAA20603.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066032(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066032 NCBI Caenorhabditis elegans F23F12.3 (F23F12.3) mRNA, complete cds. 11/11 None
GENEFINDER00000039917 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:6505191:6508174:1 11/11 None
SNAP00000039908 ENSEMBL cdna:SNAP chromosome:CEL140:III:6505949:6507560:1 11/11 None
F23F12.3 ENSEMBL cdna:known chromosome:CEL140:III:6500394:6507652:1 gene:F23F12.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:6505847-6509248(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F23F12.3
Entrez Gene 175926 Entrez gene
SwissProt P46499 EMBL-EBI
WormBase CE37224 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6810 transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5215 transporter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_498433 F23F12.3 [Caenorhabditis elegans] gb|AAA20605.3| Hypothetical protein F23F12.3 [Caenorhabditis elegans] sp|P46499|YLX3_CAEEL Hypothetical protein F23F12.3 in chromosome III 0.0
blast AAY86205 Hypothetical protein F23F12.13 [Caenorhabditis elegans] 0.0
blast NP_498433 F23F12.3 [Caenorhabditis elegans] gb|AAA20605.3| Hypothetical protein F23F12.3 [Caenorhabditis elegans] sp|P46499|YLX3_CAEEL Hypothetical protein F23F12.3 in chromosome III 1.0E-173
blast CAE73649 Hypothetical protein CBG21150 [Caenorhabditis briggsae] 1.0E-144
blast AAY86205 Hypothetical protein F23F12.13 [Caenorhabditis elegans] 1.0E-124
blast CAE73649 Hypothetical protein CBG21150 [Caenorhabditis briggsae] 1.0E-124
Protein Domains
Database ID Description E-Value
Pfam IPR005828 EMBL-EBI General substrate transporter 7.6E-7
Pfam IPR005828 EMBL-EBI General substrate transporter 7.6E-7
Pfam IPR005828 EMBL-EBI General substrate transporter 9.5E-9
Pfam IPR005828 EMBL-EBI General substrate transporter 7.6E-7
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_498433.2 12 13-35,96-118,125-147,151-173,200-222,226-243,298-320,340-362,369-389,404-426,438-460,465-487
F23F12.3 12 13-35,96-118,125-147,151-173,200-222,226-243,298-320,340-362,369-389,404-426,438-460,465-487
GENEFINDER00000039917 9 50-67,74-96,123-145,221-243,263-285,292-312,327-349,361-383,388-410
SNAP00000039908 7 57-79,134-156,176-198,205-225,240-262,274-296,301-323
Sequence
Target Sequence 
>C. ELEGANS:187609_S_AT
tcttcttgccccacaaatggcctatctttctgaaatttatccaccaattccttacataat
cgtttgctcaatcggcattatttcattgttgatttcatgtttcttcctaccagatacaaa
aggagtcgatctcggtgctttggatcgtaatcctttggaagagtct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCTTCTTGCCCCACAAATGGCCTAT 532 615 597 Antisense
CCCACAAATGGCCTATCTTTCTGAA 207 259 606 Antisense
TATCTTTCTGAAATTTATCCACCAA 290 659 619 Antisense
TATCCACCAATTCCTTACATAATCG 233 657 634 Antisense
ATTCCTTACATAATCGTTTGCTCAA 144 9 643 Antisense
AATCGTTTGCTCAATCGGCATTATT 257 169 654 Antisense
TCGGCATTATTTCATTGTTGATTTC 275 605 668 Antisense
TGTTGATTTCATGTTTCTTCCTACC 602 563 683 Antisense
AAGGAGTCGATCTCGGTGCTTTGGA 316 161 716 Antisense
ATCTCGGTGCTTTGGATCGTAATCC 355 33 725 Antisense
GGATCGTAATCCTTTGGAAGAGTCT 453 511 738 Antisense
Sequence Source Affymetrix Proprietary Database