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[187606_at]

Description F42A10.8 /REP_DB=WormBase Gene ID /WP=CE01299 /TR=SW:P98061 /GB=AAA19079.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 84      4grp_total > 48      6grp_cgc > 87      6grp_cgc_sub > 05      6grp_total_sub > Hat      6grp_rule_based > S5      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 76
1 2 3 4 range
A 59.6
(0.2)
A 		60.7
(0.2)
A 		95.7
(3)
A 		19.4
(-3)
A 		76
B 42.9
(-3)
A 		78.6
(1.7)
A 		88.1
(3)
A 		70.8
(0.7)
A 		45
C 37.9
(-3)
A 		93.9
(3)
P 		72.3
(0.7)
P 		87.3
(2.3)
P 		56
avr 46.8
(-3) 		77.7
(1.8) 		85.4
(3) 		59.2
(-1.1) 		39

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 849
1 2 3 4 5 6 range
A 88.4
(-2.9)
A 		76.1
(-3)
P 		117.8
(-2.6)
A 		781.5
(3)
P 		753.3
(2.8)
P 		279.2
(-1.3)
P 		705
B 54.9
(-3)
A 		50.5
(-3)
A 		94.2
(-2.7)
P 		642.5
(1.2)
P 		899.6
(3)
P 		272.7
(-1.4)
P 		849
C 76.8
(-3)
A 		79.6
(-3)
P 		106.1
(-2.8)
P 		787.8
(2.8)
P 		813.4
(3)
P 		320.2
(-1)
P 		737
avr 73.4
(-3) 		68.7
(-3) 		106
(-2.7) 		737.3
(2.3) 		822.1
(3) 		290.7
(-1.2) 		753
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 24
1 2 3 4 range
A 21.7
(2.1)
A 		13.2
(-0.2)
A 		25
(3)
A 		2.8
(-3)
A 		22
B 13.7
(-3)
A 		19.4
(2.1)
A 		20.4
(3)
A 		15.1
(-1.7)
A 		7
C 12.6
(-3)
A 		27
(3)
P 		25.2
(2.3)
P 		14.7
(-2.1)
P 		14
avr 16
(-0.6) 		19.9
(1.3) 		23.5
(3) 		10.9
(-3) 		13

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 349
1 2 3 4 5 6 range
A 16.3
(-3)
A 		27.1
(-2.7)
P 		25.7
(-2.7)
A 		198.2
(2.2)
P 		227.3
(3)
P 		68
(-1.5)
P 		211
B 19.1
(-3)
A 		16.8
(-3)
A 		22.9
(-2.9)
P 		170.2
(-0.4)
P 		365.5
(3)
P 		101.7
(-1.5)
P 		349
C 28.5
(-2.9)
A 		24.3
(-3)
P 		27
(-2.9)
P 		158.4
(0.4)
P 		263.2
(3)
P 		107.2
(-0.9)
P 		239
avr 21.3
(-3) 		22.7
(-3) 		25.2
(-2.9) 		175.6
(0.5) 		285.3
(3) 		92.3
(-1.4) 		264
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187606_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8086
Sequence Type Exemplar sequence
Representative Public ID F42A10.8 NCBI
Target Description F42A10.8 /REP_DB=WormBase Gene ID /WP=CE01299 /TR=SW:P98061 /GB=AAA19079.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065941(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065941 NCBI Caenorhabditis elegans Nematode AStacin protease family member (nas-28) (nas-28) mRNA, complete cds. 11/11 None
F42A10.8 ENSEMBL cdna:known chromosome:CEL140:III:6181018:6182718:-1 gene:F42A10.8 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
AJ561217 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:6181016-6183157(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol nas-28
Ensembl F42A10.8
Entrez Gene 185658 Entrez gene
WormBase CE38958 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA19079 Nematode astacin protease protein 28 [Caenorhabditis elegans] 0.0
blast NP_498342 Nematode AStacin protease family member (nas-28) [Caenorhabditis elegans] 0.0
blast P98061 Zinc metalloproteinase nas-28 precursor (Nematode astacin 28) 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001506 EMBL-EBI Peptidase M12A, astacin 5.4E-100
Sequence
Target Sequence 
>C. ELEGANS:187606_AT
gacaacgagaggcaccagcattttcagatattatcggagtgaacaagttgtacaattgca
caagccaatgcaagatacaaatgaagtgttcaaattgtggaatcacagactcccgtaact
gtaatcaatgcaaatgtccgcgctatttcacaggagctagctgcgatagcttaccatctg
gtactgctccaaattgtaatggagccgtgcttcaggctacatcctcttgggaaaccttcg
acgcaaaagccggagacccatccagcttctcttcttccacagacaattcgaccaattgtt
actggcatatcaaagctccagaaggacaacaaattgaattcaaaatgacaaaaactccat
tggcagcaatatgtatgcaagaatgtccatggcaatcgattgaagttaatcttggaaagt
ttgacttatttggaatgataacctgctgtgacacaatcctaaaccaagtgttcacctctg
agctta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACAACGAGAGGCACCAGCATTTTC 697 363 668 Antisense
TTGTGGAATCACAGACTCCCGTAAC 65 709 762 Antisense
GACTCCCGTAACTGTAATCAATGCA 560 373 775 Antisense
ATGTCCGCGCTATTTCACAGGAGCT 228 49 801 Antisense
AGCTGCGATAGCTTACCATCTGGTA 312 81 826 Antisense
ACCATCTGGTACTGCTCCAAATTGT 372 85 840 Antisense
ATGGAGCCGTGCTTCAGGCTACATC 557 51 866 Antisense
GCTACATCCTCTTGGGAAACCTTCG 267 303 883 Antisense
TTCGACGCAAAAGCCGGAGACCCAT 339 691 904 Antisense
GGAATGATAACCTGCTGTGACACAA 690 531 1099 Antisense
AACCAAGTGTTCACCTCTGAGCTTA 447 147 1129 Antisense
Sequence Source Affymetrix Proprietary Database