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[187596_at]

Description T20B12.3 /REP_DB=WormBase Gene ID /WP=CE01409 /TR=SW:P41843 /GB=AAA19056.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 78      6grp_high1_10_10 > 97      4grp_highest_8_8 > 27      4grp_high_8_8 > 63      6grp_highest_nonorm > 84      6grp_total > 88      4grp_total > 40      6grp_highest_sub > In      6grp_high1_sub > In      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 407
1 2 3 4 range
A 729.8
(-0.1)
P 		639.6
(-3)
P 		827.2
(3)
P 		733.7
(0)
P 		188
B 673
(-1.9)
P 		632.8
(-2.9)
P 		853.9
(3)
P 		630.6
(-3)
P 		223
C 748.3
(-0)
P 		545.6
(-3)
P 		952.2
(3)
P 		684.6
(-0.9)
P 		407
avr 717
(-0.5) 		606
(-3) 		877.8
(3) 		683
(-1.3) 		272

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 440
1 2 3 4 5 6 range
A 412.1
(-3)
P 		499.4
(-1.2)
P 		482.4
(-1.5)
P 		542.1
(-0.3)
P 		591.8
(0.7)
P 		702.8
(3)
P 		291
B 447.8
(-2.2)
P 		626.6
(0.3)
P 		386.9
(-3)
P 		496.5
(-1.5)
P 		629
(0.3)
P 		826.6
(3)
P 		440
C 417.5
(-3)
P 		593.7
(-0.1)
P 		532.6
(-1.1)
P 		525.4
(-1.2)
P 		600.8
(0)
P 		778.6
(3)
P 		361
avr 425.8
(-3) 		573.2
(-0.4) 		467.3
(-2.3) 		521.3
(-1.3) 		607.2
(0.2) 		769.3
(3) 		344
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 225
1 2 3 4 range
A 265.7
(3)
P 		138.8
(-1.8)
P 		216.3
(1.1)
P 		107.4
(-3)
P 		158
B 214.6
(3)
P 		156.3
(-1.4)
P 		197.9
(1.8)
P 		134.4
(-3)
P 		80
C 247.7
(0.7)
P 		156.9
(-1.8)
P 		331.9
(3)
P 		115.4
(-3)
P 		217
avr 242.7
(2.7) 		150.7
(-1.5) 		248.7
(3) 		119.1
(-3) 		130

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 232
1 2 3 4 5 6 range
A 76
(-3)
P 		177.6
(2.9)
P 		105.1
(-1.3)
P 		137.5
(0.6)
P 		178.6
(3)
P 		171.1
(2.6)
P 		103
B 155.8
(-1.3)
P 		208.6
(0.2)
P 		94
(-3)
P 		131.5
(-1.9)
P 		255.6
(1.5)
P 		308.2
(3)
P 		214
C 154.8
(-1.1)
P 		181.2
(-0.1)
P 		135.6
(-1.8)
P 		105.7
(-3)
P 		194.4
(0.4)
P 		260.6
(3)
P 		155
avr 128.9
(-2.2) 		189.1
(0.4) 		111.6
(-3) 		124.9
(-2.4) 		209.5
(1.4) 		246.6
(3) 		135
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187596_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7279
Sequence Type Exemplar sequence
Representative Public ID T20B12.3 NCBI
Target Description T20B12.3 /REP_DB=WormBase Gene ID /WP=CE01409 /TR=SW:P41843 /GB=AAA19056.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066233(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066233 NCBI Caenorhabditis elegans T20B12.3 (T20B12.3) mRNA, complete cds. 11/11 None
SNAP00000022559 ENSEMBL cdna:SNAP chromosome:CEL140:III:7382039:7383741:1 11/11 None
GENEFINDER00000022564 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:7382039:7383741:1 11/11 None
T20B12.3.1 ENSEMBL cdna:known chromosome:CEL140:III:7382033:7383826:1 gene:T20B12.3 11/11 None
T20B12.3.2 ENSEMBL cdna:known chromosome:CEL140:III:7382035:7383826:1 gene:T20B12.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:7382036-7383739(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T20B12.3
Entrez Gene 176053 Entrez gene
SwissProt P41843 EMBL-EBI
WormBase CE01409 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA19056 Hypothetical protein T20B12.3 [Caenorhabditis elegans] ref|NP_498634.1| T20B12.3 [Caenorhabditis elegans] sp|P41843|YO93_CAEEL Hypothetical protein T20B12.3 in chromosome III 0.0
blast CAE64434 Hypothetical protein CBG09137 [Caenorhabditis briggsae] 0.0
Sequence
Target Sequence 
>C. ELEGANS:187596_AT
ccaacatacatcactgcttcgttcctcaaacgactctcccgatgccttcttcttgctcca
attgatgctcaggaaccaattcttggattaattagaaatcttgtgattagacatccaaac
tgttctgaattggttcatcgtgaggttcctcagactctttacgatgatcctttcgacaat
gatgagacagatttacacaaaactcgcgcattggaatcaagtctttgggagatgaaactt
cttcaatgtcactggaatcaaagtgtcagaaaacgagctcatttcgttgataaatcgatt
caaaaaattgagtcttacgttcgattccgatgcaccgacgagttatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCAACATACATCACTGCTTCGTTCC 174 277 919 Antisense
CTTCTTCTTGCTCCAATTGATGCTC 373 221 964 Antisense
GTGATTAGACATCCAAACTGTTCTG 334 483 1021 Antisense
GAATTGGTTCATCGTGAGGTTCCTC 617 323 1045 Antisense
TGAGGTTCCTCAGACTCTTTACGAT 169 567 1059 Antisense
GACTCTTTACGATGATCCTTTCGAC 88 373 1071 Antisense
GACAGATTTACACAAAACTCGCGCA 395 367 1104 Antisense
AACTCGCGCATTGGAATCAAGTCTT 415 141 1119 Antisense
GAAACTTCTTCAATGTCACTGGAAT 279 357 1152 Antisense
TTGAGTCTTACGTTCGATTCCGATG 239 705 1226 Antisense
GATTCCGATGCACCGACGAGTTATT 535 431 1241 Antisense
Sequence Source Affymetrix Proprietary Database