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[187595_at]

Description F23F12.8 /REP_DB=WormBase Gene ID /WP=CE01255 /TR=SW:P46504 /GB=AAA20610.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 54      4grp_total > 27      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 97
1 2 3 4 range
A 30.9
(-1.7)
A 		54.8
(-0.2)
A 		9.5
(-3)
A 		106.7
(3)
A 		97
B 11
(-3)
A 		48.6
(3)
A 		23.5
(-1)
A 		19.2
(-1.7)
A 		38
C 53.8
(2.1)
A 		43.1
(-0.2)
A 		30.2
(-3)
A 		57.9
(3)
A 		28
avr 31.9
(-1.4) 		48.8
(1.1) 		21.1
(-3) 		61.3
(3) 		40

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 66
1 2 3 4 5 6 range
A 13.7
(-2)
A 		2.5
(-3)
A 		68
(3)
A 		3.5
(-2.9)
A 		16.6
(-1.7)
A 		3.6
(-2.9)
A 		66
B 25
(-0.4)
A 		7.8
(-3)
A 		32.8
(0.8)
A 		24.4
(-0.5)
A 		9.6
(-2.7)
A 		47.3
(3)
A 		40
C 9.3
(-1)
A 		21.1
(3)
A 		9.2
(-1.1)
A 		9.5
(-1)
A 		3.6
(-3)
A 		17.8
(1.9)
A 		18
avr 16
(-1.6) 		10.5
(-2.9) 		36.7
(3) 		12.5
(-2.4) 		9.9
(-3) 		22.9
(-0.1) 		27
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 11.2
(1)
A 		11.9
(1.3)
A 		2.5
(-3)
A 		15.6
(3)
A 		13
B 3.5
(-3)
A 		12
(3)
A 		5.5
(-1.6)
A 		4.1
(-2.6)
A 		9
C 17.8
(3)
A 		12.4
(-1.1)
A 		10.5
(-2.5)
A 		9.8
(-3)
A 		8
avr 10.8
(1.7) 		12.1
(3) 		6.2
(-3) 		9.8
(0.7) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 17
1 2 3 4 5 6 range
A 2.5
(-2.3)
A 		0.9
(-3)
A 		14.8
(3)
A 		0.9
(-3)
A 		5
(-1.2)
A 		0.9
(-3)
A 		14
B 8.7
(-0.6)
A 		2.6
(-3)
A 		8
(-0.8)
A 		6.5
(-1.4)
A 		3.9
(-2.5)
A 		17.6
(3)
A 		15
C 3.5
(-0.3)
A 		6.4
(3)
A 		2.3
(-1.7)
A 		1.9
(-2.2)
A 		1.2
(-3)
A 		6
(2.5)
A 		5
avr 4.9
(-1) 		3.3
(-2.8) 		8.4
(3) 		3.1
(-3) 		3.4
(-2.7) 		8.2
(2.8) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187595_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6361
Sequence Type Exemplar sequence
Representative Public ID F23F12.8 NCBI
Target Description F23F12.8 /REP_DB=WormBase Gene ID /WP=CE01255 /TR=SW:P46504 /GB=AAA20610.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066026(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066026 NCBI Caenorhabditis elegans F23F12.8 (F23F12.8) mRNA, complete cds. 11/11 None
GENEFINDER00000039910 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:6481779:6488438:1 11/11 None
SNAP00000039901 ENSEMBL cdna:SNAP chromosome:CEL140:III:6484374:6488810:1 11/11 None
F23F12.8 ENSEMBL cdna:known chromosome:CEL140:III:6483153:6488438:1 gene:F23F12.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:6483151-6488437(+) 94.67 100.0
Public Domain and Genome References
Gene Title
Ensembl F23F12.8
Entrez Gene 184907 Entrez gene
WormBase CE38952 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_498427 F23F12.8 [Caenorhabditis elegans] sp|P46504|YLX8_CAEEL Hypothetical protein F23F12.8 in chromosome III precursor 0.0
blast AAZ32811 Hypothetical protein F23F12.8 [Caenorhabditis elegans] 0.0
blast CAE64408 Hypothetical protein CBG09100 [Caenorhabditis briggsae] 0.0
blast CAE64408 Hypothetical protein CBG09100 [Caenorhabditis briggsae] 1.0E-139
Protein Domains
Database ID Description E-Value
scop a.2.7.Seryl-tRNA synthetase (SerRS) All alpha proteins; Long alpha-hairpin; tRNA-binding arm; Seryl-tRNA synthetase (SerRS) 0.829999983310699
scop a.2.7.Seryl-tRNA synthetase (SerRS) All alpha proteins; Long alpha-hairpin; tRNA-binding arm; Seryl-tRNA synthetase (SerRS) 2.40000009536743
scop a.2.7.Seryl-tRNA synthetase (SerRS) All alpha proteins; Long alpha-hairpin; tRNA-binding arm; Seryl-tRNA synthetase (SerRS) 2.09999990463257
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_498427.1 1 7-24
F23F12.8 1 7-24
GENEFINDER00000039910 1 178-197
Sequence
Target Sequence 
>C. ELEGANS:187595_AT
tgacgttgattcgttctgtgctcatccaagatctccatcaatgtatcttcaatgtgctcc
attgtacggtaggttgggaagatggactgaaagatattgtccagatactttgatatttat
tgtatcaattggaagatgtgaaaagggagaagaaactcgtaaaccatatgatccagacaa
tcgagtcgtgattccaagactaccttcggagacttcatttgttgagtggaagggtaatag
agtgattgaccatcaactcccaactcatatatccccgccacgagtttatccgcctgtccc
ggaaactcaccaacctcaaatctacagtactattgatcagttcccaaaggatttgcttcc
aaagattccagaattagcgacagctgaaaaaacctatgctcaacaataccaaaatcacat
tcatggaagtgtggttagtggaggacataatcatcaagttgttcgaccaattgctccaac
tccaacaattgctccaaattcgaatgttccagtttcctatcaaatttctc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGACGTTGATTCGTTCTGTGCTCAT 373 571 2106 Antisense
GCTCATCCAAGATCTCCATCAATGT 173 301 2125 Antisense
AATGTGCTCCATTGTACGGTAGGTT 357 161 2156 Antisense
GAAACTCGTAAACCATATGATCCAG 4 353 2257 Antisense
GAGTCGTGATTCCAAGACTACCTTC 256 399 2288 Antisense
GACTACCTTCGGAGACTTCATTTGT 264 371 2303 Antisense
AGTGATTGACCATCAACTCCCAACT 44 61 2346 Antisense
AACTCACCAACCTCAAATCTACAGT 597 139 2409 Antisense
TCAGTTCCCAAAGGATTTGCTTCCA 681 619 2442 Antisense
ATTGCTCCAAATTCGAATGTTCCAG 494 5 2593 Antisense
GTTCCAGTTTCCTATCAAATTTCTC 350 441 2611 Antisense
Sequence Source Affymetrix Proprietary Database