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[187591_at]

Description F23F12.9 /REP_DB=WormBase Gene ID /WP=CE01256 /TR=SW:P46505 /GB=AAA20611.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 45      6grp_total > 46      4grp_total > 21      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 203
1 2 3 4 range
A 126.2
(0.8)
P 		90
(-0.4)
A 		11.2
(-3)
A 		193.3
(3)
P 		182
B 151.5
(1.8)
P 		117.5
(0.7)
P 		11
(-3)
A 		185.3
(3)
P 		174
C 196.9
(2.4)
P 		77.4
(-1.5)
M 		31.9
(-3)
A 		213.8
(3)
P 		182
avr 158.2
(1.7) 		95
(-0.4) 		18
(-3) 		197.5
(3) 		180

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 231
1 2 3 4 5 6 range
A 184.2
(-0.6)
P 		295.1
(3)
P 		194.2
(-0.3)
P 		148
(-1.8)
P 		112
(-3)
P 		276.8
(2.4)
P 		183
B 175
(-0.2)
P 		253.1
(3)
P 		217.6
(1.6)
P 		104.4
(-3)
P 		116.3
(-2.5)
P 		232.5
(2.2)
P 		149
C 184.3
(-0.4)
P 		294.6
(2.4)
P 		233.6
(0.8)
P 		231.1
(0.8)
P 		86.2
(-3)
A 		316.7
(3)
P 		231
avr 181.2
(-0.4) 		280.9
(3) 		215.1
(0.8) 		161.2
(-1.1) 		104.8
(-3) 		275.3
(2.8) 		176
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 63
1 2 3 4 range
A 45.9
(3)
P 		19.5
(-0.7)
A 		2.9
(-3)
A 		28.3
(0.5)
P 		43
B 48.3
(3)
P 		29
(0.5)
P 		2.5
(-3)
A 		39.5
(1.8)
P 		46
C 65.2
(3)
P 		22.3
(-1.8)
M 		11.1
(-3)
A 		36.1
(-0.2)
P 		54
avr 53.1
(3) 		23.6
(-0.7) 		5.5
(-3) 		34.6
(0.7) 		48

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 78
1 2 3 4 5 6 range
A 34
(-3)
P 		104.9
(3)
P 		42.3
(-2.3)
P 		37.5
(-2.7)
P 		33.8
(-3)
P 		67.4
(-0.2)
P 		71
B 60.9
(0.4)
P 		84.2
(2.7)
P 		52.8
(-0.4)
P 		27.7
(-3)
P 		47.3
(-1)
P 		86.7
(3)
P 		59
C 68.3
(0.1)
P 		89.9
(1.8)
P 		59.5
(-0.6)
P 		46.5
(-1.6)
P 		27.9
(-3)
A 		106
(3)
P 		78
avr 54.4
(-1.1) 		93
(3) 		51.5
(-1.4) 		37.2
(-2.9) 		36.3
(-3) 		86.7
(2.3) 		57
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187591_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6256
Sequence Type Exemplar sequence
Representative Public ID F23F12.9 NCBI
Target Description F23F12.9 /REP_DB=WormBase Gene ID /WP=CE01256 /TR=SW:P46505 /GB=AAA20611.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066025(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066025 NCBI Caenorhabditis elegans F23F12.9a (F23F12.9) mRNA, complete cds. 11/11 None
GENEFINDER00000039915 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:6478915:6480881:1 11/11 None
F23F12.9a ENSEMBL cdna:known chromosome:CEL140:III:6478895:6481161:1 gene:F23F12.9 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_181936 8/11 Cross Hyb Matching Probes None
SNAP00000039900 5/11 Cross Hyb Matching Probes None
F23F12.9b 8/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
175924 2 1 NM_066025 171733_x_at (10), 187591_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:6478913-6480880(+) 100.0 100.0
Public Domain and Genome References
Ensembl F23F12.9
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_498426 F23F12.9a [Caenorhabditis elegans] gb|AAA20611.1| Hypothetical protein F23F12.9a [Caenorhabditis elegans] sp|P46505|YLX9_CAEEL Hypothetical protein F23F12.9 in chromosome III 5.0E-91
blast NP_871665 F23F12.9b [Caenorhabditis elegans] gb|AAN63409.1| Hypothetical protein F23F12.9b [Caenorhabditis elegans] 2.0E-26
blast CAE64411 Hypothetical protein CBG09103 [Caenorhabditis briggsae] 8.0E-12
Sequence
Target Sequence 
>C. ELEGANS:187591_AT
acaccatgcaaggagcacactacccgcaaatggcattcgatccaacatggcaaatgcatc
cagcgtacgcaactgcaactgcttttaatccatacaatgtatcattcgacttcacacgct
ctccaaatacctccggcggatccgatgaatcaatgagtgatggcagcaagatcgacccaa
aacgatctccaaaatatcttgaaaagcgtatgaagaataatgaggctgcaaagaagtcac
gagcctccagaaaacatcgagagcagaagaatcagacagagaatgagttactcaaaagga
aaaacgcagcattagaggaagaactgaagcaagcaaaatgtgaattggctcagatgcaaa
tcacgatccga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACACCATGCAAGGAGCACACTACCC 685 109 20 Antisense
CACACTACCCGCAAATGGCATTCGA 170 195 35 Antisense
AATGGCATTCGATCCAACATGGCAA 393 161 48 Antisense
ATGGCAAATGCATCCAGCGTACGCA 207 53 66 Antisense
AGCGTACGCAACTGCAACTGCTTTT 191 79 81 Antisense
GCAACTGCTTTTAATCCATACAATG 641 319 94 Antisense
AATGTATCATTCGACTTCACACGCT 133 165 115 Antisense
GAGTGATGGCAGCAAGATCGACCCA 560 401 174 Antisense
TGCAAAGAAGTCACGAGCCTCCAGA 666 575 246 Antisense
GAGCCTCCAGAAAACATCGAGAGCA 94 385 260 Antisense
GGCTCAGATGCAAATCACGATCCGA 473 539 366 Antisense
Sequence Source Affymetrix Proprietary Database