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[187590_at]

Description C34E10.5 /REP_DB=WormBase Gene ID /WP=CE26881 /TR=SW:P46580 /GB=AAA19067.2 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 76      6grp_high1_10_10 > 66      4grp_highest_8_8 > 12      4grp_high_8_8 > 31      6grp_highest_nonorm > 92      6grp_total > 67      4grp_total > 32      6grp_highest_sub > Da      6grp_cgc > 47      6grp_high1_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 412
1 2 3 4 range
A 338.6
(-3)
P 		388.5
(-0.5)
P 		458
(3)
P 		412.8
(0.7)
P 		119
B 238.3
(-3)
P 		476.1
(0.5)
P 		387.5
(-0.8)
P 		650.4
(3)
P 		412
C 295.5
(-2.9)
P 		371.7
(-0.1)
P 		455.6
(3)
P 		293.8
(-3)
P 		162
avr 290.8
(-3) 		412.1
(1.5) 		433.7
(2.3) 		452.3
(3) 		162

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 625
1 2 3 4 5 6 range
A 265.2
(-1.2)
P 		285.8
(-0.8)
P 		235.7
(-1.7)
P 		268.9
(-1.1)
P 		163
(-3)
P 		496.1
(3)
P 		333
B 234.9
(-2.4)
P 		170.3
(-3)
P 		269.1
(-2)
P 		276.4
(-2)
P 		207.4
(-2.6)
P 		777.2
(3)
P 		607
C 256.3
(-2.5)
P 		219.4
(-2.8)
P 		302
(-2)
P 		247.7
(-2.5)
P 		203.5
(-3)
P 		788.2
(3)
P 		585
avr 252.1
(-2.3) 		225.2
(-2.6) 		268.9
(-2.1) 		264.3
(-2.1) 		191.3
(-3) 		687.2
(3) 		496
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 109
1 2 3 4 range
A 123.3
(3)
P 		84.3
(-0.7)
P 		119.7
(2.7)
P 		60.4
(-3)
P 		63
B 76
(-3)
P 		117.6
(1)
P 		89.8
(-1.7)
P 		138.6
(3)
P 		63
C 97.8
(-0.3)
P 		106.9
(0.2)
P 		158.8
(3)
P 		49.5
(-3)
P 		109
avr 99
(-0.6) 		102.9
(0) 		122.8
(3) 		82.8
(-3) 		40

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 241
1 2 3 4 5 6 range
A 48.9
(-3)
P 		101.6
(1.4)
P 		51.3
(-2.8)
P 		68.2
(-1.4)
P 		49.2
(-3)
P 		120.8
(3)
P 		72
B 81.7
(-2.4)
P 		56.7
(-3)
P 		65.3
(-2.8)
P 		73.2
(-2.6)
P 		84.3
(-2.3)
P 		289.8
(3)
P 		233
C 95
(-1.7)
P 		66.9
(-2.5)
P 		76.9
(-2.2)
P 		49.8
(-3)
P 		65.9
(-2.5)
P 		263.9
(3)
P 		214
avr 75.2
(-2.6) 		75.1
(-2.6) 		64.5
(-3) 		63.7
(-3) 		66.5
(-2.9) 		224.8
(3) 		161
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187590_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7894
Sequence Type Exemplar sequence
Representative Public ID C34E10.5 NCBI
Target Description C34E10.5 /REP_DB=WormBase Gene ID /WP=CE26881 /TR=SW:P46580 /GB=AAA19067.2 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026029(11), NM_001026030(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026029 NCBI Caenorhabditis elegans tryptophanyl (W) tRNA Synthetase family member (wrs-2) (wrs-2) mRNA, complete cds. 11/11 None
NM_001026030 NCBI Caenorhabditis elegans tryptophanyl (W) tRNA Synthetase family member (wrs-2) (wrs-2) mRNA, complete cds. 11/11 None
SNAP00000034355 ENSEMBL cdna:SNAP chromosome:CEL140:III:5236388:5239884:1 11/11 None
GENEFINDER00000034364 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:5236388:5239884:1 11/11 None
C34E10.4a ENSEMBL cdna:known chromosome:CEL140:III:5235911:5239985:1 gene:C34E10.4 11/11 None
C34E10.4b ENSEMBL cdna:known chromosome:CEL140:III:5236849:5239884:1 gene:C34E10.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:5230730-5238092(+) 99.45 100.0
Public Domain and Genome References
Ensembl C34E10.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA19066 Tryptophanyl (w) trna synthetase protein 2, isoform a [Caenorhabditis elegans] ref|NP_001021200.1| tryptophanyl (W) tRNA Synthetase family member (wrs-2) [Caenorhabditis elegans] sp|P46579|SYWM_CAEEL Probable tryptophanyl-tRNA synthetase, mitochondrial (Tryptophan--tRNA ligase) (TrpRS) 0.0
blast CAE73661 Hypothetical protein CBG21169 [Caenorhabditis briggsae] 0.0
blast AAV34793 Tryptophanyl (w) trna synthetase protein 2, isoform b [Caenorhabditis elegans] ref|NP_001021201.1| tryptophanyl (W) tRNA Synthetase family member (wrs-2) [Caenorhabditis elegans] dbj|BAC76734.1| tryptophanyl-tRNA synthetase (mitochondrial) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001841 EMBL-EBI Zn-finger, RING 3.8E-9
Pfam IPR006845 EMBL-EBI Pex2 / Pex12, N-terminal 2.8E-67
Pfam IPR002305 EMBL-EBI Aminoacyl-tRNA synthetase, class Ib 9.6E-98
Pfam IPR002305 EMBL-EBI Aminoacyl-tRNA synthetase, class Ib 9.6E-98
Pfam IPR002305 EMBL-EBI Aminoacyl-tRNA synthetase, class Ib 9.6E-98
Sequence
Target Sequence 
>C. ELEGANS:187590_AT
agaatcaacacttacacatccatcaactgaatcatttcttggaattccgattcgaaaaaa
tcaaaaagctcgtcaatcatttcttgacgtgtttttctggttgagaactaaactttttcc
acaacttcaacgtgctcatattgctcttttctatataactggagcatattatagcatcgc
cagaagattcactggaattcgatttttatcagccagtgctcattcggatattcctgcgct
gaaagtttatcgattccttggttatataacattaattcaattggcagttagtattggaat
atcattatattcatttctagaacaggagaaattcaataataaattgaaaaaggagaaaaa
agaaaacaacggaggatcggataggaatctagacgagaattcgctatttcatccaacatt
ccagtgttcaatttgtcttgaaaacaagaatccatctgctcttttctgtggacatttatt
ctgttggacgtgtattcaggagcacgcagtagctgccacgtcatccgcttcaacttcatc
tgctcgttgcccacagtgtcgattggaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGAATCAACACTTACACATCCATCA 5 77 3204 Antisense
CATTTCTTGACGTGTTTTTCTGGTT 678 213 3281 Antisense
AACGTGCTCATATTGCTCTTTTCTA 167 145 3332 Antisense
GGAGCATATTATAGCATCGCCAGAA 314 521 3364 Antisense
GATTTTTATCAGCCAGTGCTCATTC 431 429 3404 Antisense
AGTGCTCATTCGGATATTCCTGCGC 464 59 3418 Antisense
ATTCCTGCGCTGAAAGTTTATCGAT 291 9 3433 Antisense
AATTCGCTATTTCATCCAACATTCC 678 177 3601 Antisense
CAAGAATCCATCTGCTCTTTTCTGT 687 185 3648 Antisense
CAGGAGCACGCAGTAGCTGCCACGT 647 205 3700 Antisense
TCGTTGCCCACAGTGTCGATTGGAA 635 603 3747 Antisense
Sequence Source Affymetrix Proprietary Database