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[187584_at]

Description F21H12.1 /REP_DB=WormBase Gene ID /WP=CE27989 /TR=SW:Q09309 /GB=AAC46713.2 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 70      4grp_highest_8_8 > 60      4grp_high_8_8 > 26      6grp_total > 03      4grp_total > 37      6grp_cgc > 51      6grp_high1_sub > 04      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 135
1 2 3 4 range
A 245.5
(3)
P 		240.4
(2.5)
P 		189
(-3)
P 		219.3
(0.2)
P 		57
B 214.4
(-2.7)
P 		231.1
(3)
P 		218.1
(-1.4)
P 		213.4
(-3)
P 		18
C 323.7
(3)
P 		235.3
(-1.3)
P 		199.4
(-3)
P 		247.4
(-0.7)
P 		124
avr 261.2
(3) 		235.6
(0.4) 		202.2
(-3) 		226.7
(-0.5) 		59

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 203
1 2 3 4 5 6 range
A 243.6
(3)
P 		177.2
(-1.3)
P 		191.2
(-0.4)
P 		150.5
(-3)
M 		229.7
(2.1)
P 		175.9
(-1.4)
A 		93
B 187.8
(2.9)
P 		189.5
(3)
A 		155.6
(1.6)
M 		185.8
(2.9)
P 		161
(1.9)
P 		40.4
(-3)
P 		149
C 174.9
(-2.6)
P 		219.4
(1.4)
P 		173.4
(-2.8)
P 		237.1
(3)
P 		192.2
(-1.1)
P 		170.8
(-3)
P 		66
avr 202.1
(3) 		195.4
(2.5) 		173.4
(0.6) 		191.1
(2.1) 		194.3
(2.4) 		129
(-3) 		73
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 75
1 2 3 4 range
A 89.4
(3)
P 		52.2
(-0.9)
P 		49.4
(-1.2)
P 		32.1
(-3)
P 		57
B 68.4
(3)
P 		57.1
(0)
P 		50.5
(-1.7)
P 		45.5
(-3)
P 		23
C 107.2
(3)
P 		67.7
(-0.6)
P 		69.5
(-0.5)
P 		41.7
(-3)
P 		66
avr 88.3
(3) 		59
(-0.6) 		56.5
(-0.9) 		39.8
(-3) 		49

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 54
1 2 3 4 5 6 range
A 44.9
(-1.7)
P 		63
(1.8)
P 		41.6
(-2.3)
P 		38.2
(-3)
M 		69.3
(3)
P 		42.8
(-2.1)
A 		31
B 65.4
(3)
P 		63.1
(2.7)
A 		37.8
(-0.3)
M 		49.2
(1.1)
P 		65.4
(3)
P 		15.1
(-3)
P 		50
C 64.8
(2.4)
P 		66.9
(3)
P 		44.1
(-3)
P 		47.7
(-2.1)
P 		62.2
(1.8)
P 		57.2
(0.4)
P 		23
avr 58.4
(1.4) 		64.3
(2.7) 		41.2
(-2.4) 		45
(-1.5) 		65.6
(3) 		38.4
(-3) 		27
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187584_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4093
Sequence Type Exemplar sequence
Representative Public ID F21H12.1 NCBI
Target Description F21H12.1 /REP_DB=WormBase Gene ID /WP=CE27989 /TR=SW:Q09309 /GB=AAC46713.2 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062821, NM_062822
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
GENEFINDER00000015303 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:6097786:6099538:1 None
NM_062822 NCBI Caenorhabditis elegans Regulator of G protein Signaling family member (rgs-3) (rgs-3) mRNA, complete cds. A 
F21H12.1 ENSEMBL cdna:known chromosome:CEL140:II:6097785:6099565:1 gene:F21H12.1 None
GENEFINDER00000038450 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:6100019:6106358:1 None
C29H12.3a ENSEMBL cdna:known chromosome:CEL140:II:6100015:6110036:1 gene:C29H12.3 A 
SNAP00000015300 ENSEMBL cdna:SNAP chromosome:CEL140:II:6097786:6099538:1 None
NM_062821 NCBI Caenorhabditis elegans F21H12.1 (F21H12.1) mRNA, complete cds. None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:6097782-6101830(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F21H12.1
C29H12.3
Entrez Gene 174019 Entrez gene
SwissProt Q09309 EMBL-EBI
WormBase CE32634 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:256967_AT transducin family protein / WD-40 repeat family protein at
CANINE_2:CFAAFFX.15661.1.S1_AT similar to retinoblastoma binding protein 5 cfa
DROSOPHILA_2:1627743_AT dm
DROSOPHILA_2:1626046_S_AT dm
DROSGENOME1:149118_AT dm
CHICKEN:GGA.17072.1.S1_S_AT retinoblastoma binding protein 5 gga
CHICKEN:GGAAFFX.11714.1.S1_AT retinoblastoma binding protein 5 gga
CHICKEN:GGAAFFX.11714.1.S1_S_AT retinoblastoma binding protein 5 gga
HG-U133_PLUS_2:205169_AT retinoblastoma binding protein 5 hs
HG-U133A:205169_AT retinoblastoma binding protein 5 hs
HG-FOCUS:205169_AT retinoblastoma binding protein 5 hs
HG-U133A_2:205169_AT retinoblastoma binding protein 5 hs
HU35KSUBA:HUM_ALU_AT retinoblastoma binding protein 5 hs
HU35KSUBB:HUM_ALU_AT retinoblastoma binding protein 5 hs
HU35KSUBC:HUM_ALU_AT retinoblastoma binding protein 5 hs
HU35KSUBD:HUM_ALU_AT retinoblastoma binding protein 5 hs
HUGENEFL:HUM_ALU_AT retinoblastoma binding protein 5 hs
HC-G110:HUM_ALU_AT retinoblastoma binding protein 5 hs
HU35KSUBD:RC_AA176604_R_AT retinoblastoma binding protein 5 hs
HUGENEFL:X85134_RNA1_AT retinoblastoma binding protein 5 hs
U133_X3P:G4826969_3P_X_AT retinoblastoma binding protein 5 hs
U133_X3P:G4826969_3P_AT retinoblastoma binding protein 5 hs
U133_X3P:205169_3P_AT retinoblastoma binding protein 5 hs
HG-U95AV2:36769_AT retinoblastoma binding protein 5 hs
HG-U95C:62692_AT retinoblastoma binding protein 5 hs
HG-U95E:71534_AT retinoblastoma binding protein 5 hs
MG-U74BV2:109971_AT retinoblastoma binding protein 5 mm
MG-U74BV2:165072_F_AT retinoblastoma binding protein 5 mm
MOE430B:1428886_AT retinoblastoma binding protein 5 mm
MOUSE430_2:1428886_AT retinoblastoma binding protein 5 mm
MU19KSUBC:TC35598_AT Retinoblastoma binding protein 5, mRNA (cDNA clone IMAGE:6832882) mm
MU19KSUBC:TC36145_AT Retinoblastoma binding protein 5, mRNA (cDNA clone IMAGE:6832882) mm
MU19KSUBC:TC36145_G_AT Retinoblastoma binding protein 5, mRNA (cDNA clone IMAGE:6832882) mm
RG-U34B:RC_AI059731_AT retinoblastoma binding protein 5 (predicted) rn
RG-U34C:RC_AI071244_AT retinoblastoma binding protein 5 (predicted) rn
RAE230B:1381323_AT retinoblastoma binding protein 5 (predicted) rn
RAT230_2:1381323_AT retinoblastoma binding protein 5 (predicted) rn
YEAST_2:1778119_AT Subunit of the COMPASS (Set1C) complex, which methylates Rad6p ubiquitinated histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member with similarity to mammalian Rbbp7 Sc
Protein Similarities
Method ID Description E-Value
blast NP_495222 F21H12.1 [Caenorhabditis elegans] gb|AAC46713.4| Hypothetical protein F21H12.1 [Caenorhabditis elegans] sp|Q09309|YQS1_CAEEL Hypothetical WD-repeat protein F21H12.1 in chromosome II 0.0
blast T16130 hypothetical protein F21H12.1 - Caenorhabditis elegans 0.0
blast CAE59247 Hypothetical protein CBG02573 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 6.7E-4
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 4.6E-9
Pfam IPR000342 EMBL-EBI Regulator of G protein 6.8E-10
Pfam IPR000342 EMBL-EBI Regulator of G protein 5.6E-25
Sequence
Target Sequence 
>C. ELEGANS:187584_AT
gctcttctagatgttcagtggcacccaactcgtccaataatattatcaattgcgcaagga
accgtttcaatgtggacccaagctcatgttgaaaactggtctgcatttgctccagaattt
caagaactggaagaaaacgaaaagtatattgagaaagaaaatgaatttgatatggaagat
gaggatgccgatgaagacatgactagtaaaagtcaagatgcagaagatgatattattgat
gtagtcaatgttcggcctgaagattaccttgctagttcggatgaggaagattatattcga
aatttggacaatcttctgaatccagcatgggtggcatctgcaaaaggatatgaactttca
ccaaaatcaaagaatatgtggcgctcctacaaggctgaaactccggaacttgctgatgaa
actcaagaagttgatctcaatcttcatgacagctctgaatctgatcacgaaggttcgagt
tcccccttttgcgatgtcccacttctcattatacattgtt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTCTTCTAGATGTTCAGTGGCACC 363 301 898 Antisense
GCACCCAACTCGTCCAATAATATTA 255 315 918 Antisense
GAACCGTTTCAATGTGGACCCAAGC 428 327 956 Antisense
ATGTGGACCCAAGCTCATGTTGAAA 430 47 967 Antisense
TGAAAACTGGTCTGCATTTGCTCCA 294 573 987 Antisense
ATCAAAGAATATGTGGCGCTCCTAC 510 27 1263 Antisense
TGGCGCTCCTACAAGGCTGAAACTC 347 543 1276 Antisense
GCTGAAACTCCGGAACTTGCTGATG 195 297 1291 Antisense
GATCTCAATCTTCATGACAGCTCTG 652 419 1330 Antisense
TGATCACGAAGGTTCGAGTTCCCCC 609 563 1359 Antisense
GTCCCACTTCTCATTATACATTGTT 619 475 1393 Antisense
Sequence Source Affymetrix Proprietary Database