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[187569_s_at]

Description T20F5.3 /REP_DB=WormBase Gene ID /WP=CE13820 /TR=SW:P91478 /GB=AAB37664.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 57      6grp_high1_10_10 > 85      4grp_highest_8_8 > 09      4grp_high_8_8 > 54      6grp_highest_nonorm > 72      6grp_total > 65      4grp_total > 58      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 303
1 2 3 4 range
A 313.1
(-1.6)
P
527.5
(3)
P
484.1
(2.1)
P
247.9
(-3)
P
280
B 352.6
(-1.3)
P
503.5
(2)
P
551
(3)
P
276
(-3)
P
275
C 369.1
(-1.8)
P
525.1
(2.6)
P
540.9
(3)
P
324.9
(-3)
P
216
avr 344.9
(-1.5)
518.7
(2.8)
525.3
(3)
282.9
(-3)
242

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 219
1 2 3 4 5 6 range
A 340
(-3)
P
433.8
(0)
P
527.2
(3)
P
420.3
(-0.4)
P
428.5
(-0.2)
P
426.3
(-0.2)
P
187
B 336.1
(-3)
P
454.7
(0.9)
P
473.7
(1.5)
P
409.1
(-0.6)
P
506.4
(2.6)
P
519.3
(3)
P
183
C 399.8
(-2.1)
P
418.2
(-1.5)
P
388.8
(-2.5)
P
477.3
(0.4)
P
374.1
(-3)
P
554.7
(3)
P
181
avr 358.6
(-3)
435.6
(0.3)
463.2
(1.4)
435.6
(0.3)
436.3
(0.3)
500.1
(3)
142
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 152
1 2 3 4 range
A 114
(2.2)
P
114.5
(2.2)
P
126.5
(3)
P
36.3
(-3)
P
90
B 112.4
(1.7)
P
124.3
(2.7)
P
127.7
(3)
P
58.8
(-3)
P
69
C 122.2
(0)
P
151
(1.3)
P
188.5
(3)
P
54.8
(-3)
P
134
avr 116.2
(1.1)
129.9
(1.9)
147.6
(3)
50
(-3)
98

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 143
1 2 3 4 5 6 range
A 62.7
(-3)
P
154.3
(3)
P
114.8
(0.4)
P
106.6
(-0.1)
P
129.3
(1.4)
P
103.8
(-0.3)
P
92
B 116.9
(-2.5)
P
151.4
(-0.4)
P
115
(-2.6)
P
108.4
(-3)
P
205.8
(3)
P
193.7
(2.3)
P
97
C 148.2
(0.5)
P
127.6
(-0.9)
P
99
(-2.8)
P
96
(-3)
P
121
(-1.3)
P
185.7
(3)
P
90
avr 109.3
(-2.4)
144.4
(1.3)
109.6
(-2.4)
103.7
(-3)
152
(2)
161.1
(3)
57
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 187569_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.98
Sequence Type Exemplar sequence
Representative Public ID T20F5.3 NCBI
Target Description T20F5.3 /REP_DB=WormBase Gene ID /WP=CE13820 /TR=SW:P91478 /GB=AAB37664.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058861(11)
Transcript Assignments
Representative
Transcript
Description Matching Probes Related Probesets by Grade
NM_058861 NCBI Caenorhabditis elegans T20F5.3 (T20F5.3) mRNA, complete cds. 11/11 None
SNAP00000034147 ENSEMBL cdna:SNAP chromosome:CEL140:I:3909688:3910539:-1 11/11 None
GENEFINDER00000034157 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:3909688:3912473:-1 11/11 None
T20F5.3 ENSEMBL cdna:known chromosome:CEL140:I:3909648:3912473:-1 gene:T20F5.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:3909687-3912473(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T20F5.3
Entrez Gene 171976 Entrez gene
SwissProt P91478 EMBL-EBI
WormBase CE13820 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6412 protein biosynthesis inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB37664 Hypothetical protein T20F5.3 [Caenorhabditis elegans] ref|NP_491262.1| T20F5.3 [Caenorhabditis elegans] sp|P91478|YOK3_CAEEL Hypothetical protein T20F5.3 in chromosome I 1.0E-172
blast AAB37664 Hypothetical protein T20F5.3 [Caenorhabditis elegans] ref|NP_491262.1| T20F5.3 [Caenorhabditis elegans] sp|P91478|YOK3_CAEEL Hypothetical protein T20F5.3 in chromosome I 1.0E-113
blast CAE66697 Hypothetical protein CBG12038 [Caenorhabditis briggsae] 1.0E-76
blast CAE66697 Hypothetical protein CBG12038 [Caenorhabditis briggsae] 2.0E-76
blast EAA04181 ENSANGP00000012806 [Anopheles gambiae str. PEST] ref|XP_308944.2| ENSANGP00000012806 [Anopheles gambiae str. PEST] 3.0E-11
blast EAA04181 ENSANGP00000012806 [Anopheles gambiae str. PEST] ref|XP_308944.2| ENSANGP00000012806 [Anopheles gambiae str. PEST] 7.0E-11
Protein Domains
Database ID Description E-Value
Pfam IPR002661 EMBL-EBI Ribosome recycling factor 5.3E-62
Pfam IPR002661 EMBL-EBI Ribosome recycling factor 5.3E-62
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_491262.1 1 52-74
T20F5.3 1 52-74
GENEFINDER00000034157 1 52-74
Sequence
Target Sequence
>C. ELEGANS:187569_S_AT
atccaccagcagttttctcgaatttggaggaaaacgcagtcgtccaggaaacaataaaag
aaattcaaagagttgaaggattacttgtcgaagagctcacaagacatttttctcttaaag
tagatattcggcaatatgaagatgtcatggtcaagcttgaaaatggaaaggataagccac
tgtccatgattgccagagtcacattaaaatctccacttatgattatgatcaacttccagg
ataatccatctgcaattaaagcagcaaaattggcgattcagaaatcaactcttaatgtaa
ctccacaacaggagggagctgttttgtatgtgaatgtgccgccgatgagtaaagaaagaa
gagagaaaatggcatctgatgcgaagggacgaattctcaatgaatacaaaaaggcgatca
acgaaatctattccaaaagtgataagaagtcgtcgaatgagttttccacgagaccagatg
aagccaagaaaacacgggaagcactattgaatatgaagcacgctgctgaacaaagaggag
gtctactcatcgaagaacgc


BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position
Strandedness
ATCCACCAGCAGTTTTCTCGAATTT 101 41 533 Antisense
GGAGGAAAACGCAGTCGTCCAGGAA 221 489 558 Antisense
GAGCTCACAAGACATTTTTCTCTTA 402 387 625 Antisense
GCCACTGTCCATGATTGCCAGAGTC 503 279 708 Antisense
GAGTCACATTAAAATCTCCACTTAT 230 399 728 Antisense
GATCAACTTCCAGGATAATCCATCT 533 421 759 Antisense
AATCAACTCTTAATGTAACTCCACA 109 171 815 Antisense
AGGAGGGAGCTGTTTTGTATGTGAA 411 55 842 Antisense
TGTATGTGAATGTGCCGCCGATGAG 409 557 857 Antisense
GAATGAGTTTTCCACGAGACCAGAT 572 331 987 Antisense
AGGAGGTCTACTCATCGAAGAACGC 168 55 1068 Antisense
Sequence Source Affymetrix Proprietary Database