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[187561_s_at] ATPase

Description C17H12.14 /REP_DB=WormBase Gene ID /WP=CE19362 /GB=AAK67210.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=ATPase
Cluster 6grp_highest_10_10 > 33      6grp_high1_10_10 > 22      4grp_highest_8_8 > 25      4grp_high_8_8 > 60      6grp_highest_nonorm > 16      6grp_total > 22      4grp_total > 60      6grp_highest_sub > 02      6grp_cgc > 61      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Hat      6grp_rule_based > S2      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 7537
1 2 3 4 range
A 9441.6
(1.2)
P 		10719.1
(2.3)
P 		11560.3
(3)
P 		4604.5
(-3)
P 		6956
B 8315.4
(0.6)
P 		10367.4
(2.7)
P 		10696.6
(3)
P 		4723.4
(-3)
P 		5973
C 9053.2
(2.3)
P 		8070.3
(1.3)
P 		9721
(3)
P 		4023.2
(-3)
P 		5698
avr 8936.7
(1.3) 		9718.9
(2.1) 		10659.3
(3) 		4450.4
(-3) 		6209

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 5240
1 2 3 4 5 6 range
A 8063.7
(2.2)
P 		8720.6
(3)
P 		7890.2
(2)
P 		6900.5
(0.9)
P 		5191.9
(-1.1)
P 		3569.6
(-3)
P 		5151
B 8003.4
(2.3)
P 		8228.4
(2.6)
P 		8518.9
(3)
P 		7106.3
(1.2)
P 		5212.7
(-1.2)
P 		3804.4
(-3)
P 		4715
C 6710.7
(0.3)
P 		8809.5
(3)
P 		8533.6
(2.6)
P 		7057
(0.8)
P 		4719.3
(-2.2)
P 		4127.4
(-3)
P 		4682
avr 7592.6
(1.7) 		8586.2
(3) 		8314.2
(2.7) 		7021.3
(1) 		5041.3
(-1.5) 		3833.8
(-3) 		4752
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2764
1 2 3 4 range
A 3437.7
(3)
P 		2326.8
(0.6)
P 		3022.1
(2.1)
P 		674
(-3)
P 		2764
B 2651.7
(3)
P 		2560.5
(2.7)
P 		2478.7
(2.4)
P 		1006.6
(-3)
P 		1645
C 2996.9
(2.1)
P 		2320.3
(0.6)
P 		3388.1
(3)
P 		678.4
(-3)
P 		2710
avr 3028.8
(3) 		2402.5
(1.3) 		2963
(2.8) 		786.3
(-3) 		2243

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2232
1 2 3 4 5 6 range
A 1486.5
(-1.3)
P 		3101.5
(3)
P 		1718.3
(-0.7)
P 		1750.1
(-0.6)
P 		1566.7
(-1.1)
P 		869.1
(-3)
P 		2232
B 2784.8
(3)
P 		2738.9
(2.8)
P 		2068.7
(-0.1)
P 		1882.2
(-1)
P 		2117.9
(0.1)
P 		1418.6
(-3)
P 		1366
C 2487.4
(2.1)
P 		2688
(3)
P 		2171.9
(0.6)
P 		1419.2
(-2.8)
P 		1527.1
(-2.3)
P 		1381.7
(-3)
P 		1306
avr 2252.9
(0.8) 		2842.8
(3) 		1986.3
(-0.2) 		1683.8
(-1.3) 		1737.2
(-1.1) 		1223.1
(-3) 		1620
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 187561_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9024
Sequence Type Exemplar sequence
Representative Public ID C17H12.14 NCBI
Target Description C17H12.14 /REP_DB=WormBase Gene ID /WP=CE19362 /GB=AAK67210.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=ATPase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068639(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068639 NCBI Caenorhabditis elegans C17H12.14 (ATPase) mRNA, complete cds. 11/11 None
SNAP00000015106 ENSEMBL cdna:SNAP chromosome:CEL140:IV:6791082:6792010:1 11/11 None
GENEFINDER00000015121 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:6791082:6792122:1 11/11 None
C17H12.14.1 ENSEMBL cdna:known chromosome:CEL140:IV:6791056:6792548:1 gene:C17H12.14 11/11 None
C17H12.14.2 ENSEMBL cdna:known chromosome:CEL140:IV:6791058:6792548:1 gene:C17H12.14 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:6791087-6792016(+) 100.0 100.0
Public Domain and Genome References
Gene Title ATPase
Gene Symbol vha-8
Ensembl C17H12.14
Entrez Gene 177442 Entrez gene
SwissProt Q95X44 EMBL-EBI
WormBase CE19362 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:262354_AT vacuolar ATP synthase subunit E, putative / V-ATPase E subunit, putative / vacuolar proton pump E subunit, putative at
CANINE:1601154_AT similar to ATPase, H+ transporting, V1 subunit E isoform 1 cfa
CANINE:1599371_AT similar to ATPase, H+ transporting, V1 subunit E isoform 1 cfa
CANINE:1589967_S_AT similar to ATPase, H+ transporting, V1 subunit E isoform 1 cfa
CANINE_2:CFA.1302.1.S1_AT similar to ATPase, H+ transporting, V1 subunit E isoform 1 cfa
CANINE_2:CFAAFFX.24784.1.S1_S_AT similar to ATPase, H+ transporting, V1 subunit E isoform 1 cfa
DROSOPHILA_2:1629065_S_AT Vacuolar H+-ATPase 26kD E subunit dm
DROSGENOME1:151930_AT Vacuolar H+-ATPase 26kD E subunit dm
CHICKEN:GGAAFFX.12188.1.S1_S_AT ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E isoform 1 gga
HG-U133_PLUS_2:208678_AT ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E isoform 1 hs
HG-U133A_2:208678_AT ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E isoform 1 hs
HG-U133A:208678_AT ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E isoform 1 hs
HG-FOCUS:208678_AT ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E isoform 1 hs
HUGENEFL:X76228_AT ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E isoform 1 hs
U133_X3P:G13325247_3P_AT ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E isoform 1 hs
HG-U95AV2:37367_AT ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E isoform 1 hs
MOUSE430_2:1449711_AT ATPase, H+ transporting, V1 subunit E isoform 1 mm
MOE430A:1449711_AT ATPase, H+ transporting, V1 subunit E isoform 1 mm
MOUSE430A_2:1449711_AT ATPase, H+ transporting, V1 subunit E isoform 1 mm
MOUSE430_2:1449712_S_AT ATPase, H+ transporting, V1 subunit E isoform 1 mm
MOE430A:1449712_S_AT ATPase, H+ transporting, V1 subunit E isoform 1 mm
MOUSE430A_2:1449712_S_AT ATPase, H+ transporting, V1 subunit E isoform 1 mm
MU11KSUBA:U13841_F_AT ATPase, H+ transporting, V1 subunit E isoform 1 mm
MU11KSUBB:MSA.23749.0_F_AT ATPase, H+ transporting, V1 subunit E isoform 1 mm
MU11KSUBB:MSA.1869.0_F_AT ATPase, H+ transporting, V1 subunit E isoform 1 mm
MU11KSUBB:MSA.11239.0_F_AT ATPase, H+ transporting, V1 subunit E isoform 1 mm
MU11KSUBB:MSA.11002.0_AT ATPase, H+ transporting, V1 subunit E isoform 1 mm
MG-U74AV2:94532_AT ATPase, H+ transporting, V1 subunit E isoform 1 mm
MOE430B:1457049_AT ATPase, H+ transporting, V1 subunit E isoform 1 mm
MOUSE430_2:1457049_AT ATPase, H+ transporting, V1 subunit E isoform 1 mm
MOUSE430_2:1420038_AT V-ATPase E2 subunit mm
MOE430A:1420038_AT V-ATPase E2 subunit mm
MOUSE430A_2:1420038_AT V-ATPase E2 subunit mm
MU19KSUBB:TC26272_AT V-ATPase E2 subunit mm
RG-U34C:RC_AI169159_AT ATPase, H+ transporting, V1 subunit E isoform 1 rn
RAE230A:1371564_AT ATPase, H+ transporting, V1 subunit E isoform 1 rn
RAT230_2:1371564_AT ATPase, H+ transporting, V1 subunit E isoform 1 rn
RAE230A:1377229_AT ATPase, H+ transporting, V1 subunit E isoform 1 rn
RAT230_2:1377229_AT ATPase, H+ transporting, V1 subunit E isoform 1 rn
YEAST_2:1772572_AT Subunit E of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
15986 ATP synthesis coupled proton transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16469 proton-transporting two-sector ATPase complex inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
46933 hydrogen-transporting ATP synthase activity, rotational mechanism inferred from electronic annotation QuickGO AmiGO
46961 hydrogen-transporting ATPase activity, rotational mechanism inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK67210 Vacuolar h atpase protein 8 [Caenorhabditis elegans] ref|NP_501040.1| C17H12.14 [Caenorhabditis elegans] 1.0E-111
blast CAE61478 Hypothetical protein CBG05372 [Caenorhabditis briggsae] 1.0E-107
blast AAK67210 Vacuolar h atpase protein 8 [Caenorhabditis elegans] ref|NP_501040.1| C17H12.14 [Caenorhabditis elegans] 3.0E-99
blast CAE61478 Hypothetical protein CBG05372 [Caenorhabditis briggsae] 3.0E-95
Protein Domains
Database ID Description E-Value
Pfam IPR002842 EMBL-EBI H+-transporting two-sector ATPase, E subunit 6.4E-80
Pfam IPR002842 EMBL-EBI H+-transporting two-sector ATPase, E subunit 2.5E-71
Sequence
Target Sequence 
>C. ELEGANS:187561_S_AT
acgcaaaattcaagcctccaactctctcaacgctggacgtcttcgttgcttgaaggctcg
tgaagaccacatcggagccgtactcgacgaggctcgctcgaatctctcccgtatttccgg
agatgctgctcgttatccagctattttgaagggacttgtcatgcaaggacttcttcaatt
gctcgaaaaggaagtcgtccttcgttgccgtgagaaggatcttcgtcttgttgagcaact
tttgccagagtgccttgacggacttcaaaaggagtggggaagcaccaccaaggtcgttct
cgataaacaaaacttcttgccatcggagtctgctggaggagttgaactttctgctcgtgc
tggaaagatcaaggtgtcgtctactcttgaaagccgtttagagcttattgctaaccagat
tgtcccacaagtcagaacagctctcttcggt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACGCAAAATTCAAGCCTCCAACTCT 171 91 213 Antisense
GGACGTCTTCGTTGCTTGAAGGCTC 222 523 247 Antisense
GTACTCGACGAGGCTCGCTCGAATC 463 459 292 Antisense
GAATCTCTCCCGTATTTCCGGAGAT 156 329 312 Antisense
TCCGGAGATGCTGCTCGTTATCCAG 228 595 328 Antisense
GGATCTTCGTCTTGTTGAGCAACTT 123 513 429 Antisense
GCAACTTTTGCCAGAGTGCCTTGAC 700 319 447 Antisense
TTCTTGCCATCGGAGTCTGCTGGAG 505 689 526 Antisense
AGGAGTTGAACTTTCTGCTCGTGCT 206 57 549 Antisense
AAGGTGTCGTCTACTCTTGAAAGCC 570 159 583 Antisense
ACAAGTCAGAACAGCTCTCTTCGGT 96 113 639 Antisense
Sequence Source Affymetrix Proprietary Database