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[187557_s_at]

Description T12A2.2 /REP_DB=WormBase Gene ID /WP=CE01395 /TR=SW:P46975 /GB=AAC24442.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 30      6grp_high1_10_10 > 14      4grp_highest_8_8 > 09      4grp_high_8_8 > 61      6grp_highest_nonorm > 67      6grp_total > 05      4grp_total > 58      6grp_highest_sub > De      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1698
1 2 3 4 range
A 2042.6
(-1.5)
P 		2737.4
(3)
P 		2520.4
(1.6)
P 		1818.7
(-3)
P 		919
B 2147.3
(-0.6)
P 		2615.9
(1.1)
P 		3121.7
(3)
P 		1483.3
(-3)
P 		1638
C 2114.7
(0.4)
P 		2384.2
(1.8)
P 		2634.9
(3)
P 		1424
(-3)
P 		1211
avr 2101.5
(-0.3) 		2579.2
(2.1) 		2759
(3) 		1575.3
(-3) 		1184

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1940
1 2 3 4 5 6 range
A 1898.4
(0.1)
P 		2398.9
(3)
P 		1364.5
(-3)
P 		1867.3
(-0.1)
P 		1708.1
(-1)
P 		1703.6
(-1)
P 		1034
B 1685.5
(-0.1)
P 		2208
(3)
P 		1631.9
(-0.4)
P 		1708.9
(0.1)
P 		1522.5
(-1)
P 		1185.9
(-3)
P 		1022
C 3064.4
(3)
P 		1967.7
(-0.4)
P 		2228.7
(0.4)
P 		1260
(-2.6)
P 		2067.9
(-0.1)
P 		1124
(-3)
P 		1940
avr 2216.1
(3) 		2191.5
(2.8) 		1741.7
(-0.2) 		1612.1
(-1.1) 		1766.2
(-0.1) 		1337.8
(-3) 		878
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 678
1 2 3 4 range
A 743.7
(3)
P 		594.2
(1.1)
P 		658.9
(1.9)
P 		266.2
(-3)
P 		478
B 684.7
(2.4)
P 		646.1
(1.9)
P 		723.4
(3)
P 		316.1
(-3)
P 		407
C 700
(1.1)
P 		685.5
(0.9)
P 		918.3
(3)
P 		240.1
(-3)
P 		678
avr 709.5
(2.3) 		641.9
(1.5) 		766.9
(3) 		274.1
(-3) 		493

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 883
1 2 3 4 5 6 range
A 350
(-2.4)
P 		853.2
(3)
P 		297.2
(-3)
P 		473.6
(-1.1)
P 		515.4
(-0.6)
P 		414.8
(-1.7)
P 		556
B 586.5
(0.4)
P 		735
(3)
P 		396.3
(-3)
P 		452.6
(-2)
P 		618.6
(0.9)
P 		442.2
(-2.2)
P 		339
C 1135.9
(3)
P 		600.4
(-0.6)
P 		567.2
(-0.9)
P 		253.4
(-3)
P 		669.1
(-0.2)
P 		376.3
(-2.2)
P 		883
avr 690.8
(2.3) 		729.5
(3) 		420.2
(-2.5) 		393.2
(-3) 		601
(0.7) 		411.1
(-2.7) 		336
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 187557_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6103
Sequence Type Exemplar sequence
Representative Public ID T12A2.2 NCBI
Target Description T12A2.2 /REP_DB=WormBase Gene ID /WP=CE01395 /TR=SW:P46975 /GB=AAC24442.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065961(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065961 NCBI Caenorhabditis elegans T12A2.2 (T12A2.2) mRNA, complete cds. 11/11 None
SNAP00000026127 ENSEMBL cdna:SNAP chromosome:CEL140:III:6248586:6251183:1 11/11 None
GENEFINDER00000026136 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:6248586:6251183:1 11/11 None
T12A2.2.3 ENSEMBL cdna:known chromosome:CEL140:III:6248256:6251516:1 gene:T12A2.2 11/11 None
T12A2.2.2 ENSEMBL cdna:known chromosome:CEL140:III:6248583:6251536:1 gene:T12A2.2 11/11 None
T12A2.2.1 ENSEMBL cdna:known chromosome:CEL140:III:6248583:6251546:1 gene:T12A2.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:6248584-6251182(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T12A2.2
Entrez Gene 175886 Entrez gene
SwissProt P46975 EMBL-EBI
WormBase CE01395 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:245911_AT oligosaccharyl transferase STT3 subunit family protein at
CANINE_2:CFAAFFX.9075.1.S1_AT similar to source of immunodominant MHC-associated peptides cfa
DROSOPHILA_2:1631240_AT Oligosaccharyl transferase 3 dm
DROSGENOME1:143657_AT Oligosaccharyl transferase 3 dm
CHICKEN:GGAAFFX.12067.1.S1_AT similar to source of immunodominant MHC-associated peptides; homolog of yeast STT3 gene gga
HU35KSUBA:HUM_ALU_AT source of immunodominant MHC-associated peptides hs
HU35KSUBB:HUM_ALU_AT source of immunodominant MHC-associated peptides hs
HU35KSUBC:HUM_ALU_AT source of immunodominant MHC-associated peptides hs
HU35KSUBD:HUM_ALU_AT source of immunodominant MHC-associated peptides hs
HUGENEFL:HUM_ALU_AT source of immunodominant MHC-associated peptides hs
HC-G110:HUM_ALU_AT source of immunodominant MHC-associated peptides hs
HU35KSUBA:AA285277_AT source of immunodominant MHC-associated peptides hs
U133_X3P:HS.183454.0.A1_3P_AT source of immunodominant MHC-associated peptides hs
HG-U133_PLUS_2:224700_AT source of immunodominant MHC-associated peptides hs
HG-U133B:224700_AT source of immunodominant MHC-associated peptides hs
HG-U95C:65745_S_AT source of immunodominant MHC-associated peptides hs
HG-U133_PLUS_2:230627_AT Source of immunodominant MHC-associated peptides hs
HG-U133B:230627_AT Source of immunodominant MHC-associated peptides hs
HG-U133_PLUS_2:230798_AT Source of immunodominant MHC-associated peptides hs
HG-U133B:230798_AT Source of immunodominant MHC-associated peptides hs
HG-U133_PLUS_2:231285_AT Source of immunodominant MHC-associated peptides hs
HG-U133B:231285_AT Source of immunodominant MHC-associated peptides hs
HG-U133_PLUS_2:238303_AT Source of immunodominant MHC-associated peptides hs
HG-U133B:238303_AT Source of immunodominant MHC-associated peptides hs
HG-U95E:77047_AT Source of immunodominant MHC-associated peptides hs
HG-U95E:88755_AT Source of immunodominant MHC-associated peptides hs
HG-U95D:90479_AT Source of immunodominant MHC-associated peptides hs
HU35KSUBA:Z21244_AT Source of immunodominant MHC-associated peptides hs
U133_X3P:HS.122672.0.A1_3P_AT Source of immunodominant MHC-associated peptides hs
U133_X3P:HS.126390.0.A1_3P_AT Source of immunodominant MHC-associated peptides hs
U133_X3P:HS.143702.0.A1_3P_AT Source of immunodominant MHC-associated peptides hs
U133_X3P:HS.143702.0.A1_3P_X_AT Source of immunodominant MHC-associated peptides hs
U133_X3P:HS.160918.0.S1_3P_AT Source of immunodominant MHC-associated peptides hs
MOUSE430_2:1426342_AT RIKEN cDNA 1300006C19 gene mm
MOUSE430A_2:1426342_AT RIKEN cDNA 1300006C19 gene mm
MOE430A:1426342_AT RIKEN cDNA 1300006C19 gene mm
MOUSE430A_2:1426343_AT RIKEN cDNA 1300006C19 gene mm
MOUSE430_2:1426343_AT RIKEN cDNA 1300006C19 gene mm
MOE430A:1426343_AT RIKEN cDNA 1300006C19 gene mm
MG-U74AV2:95702_AT RIKEN cDNA 1300006C19 gene mm
MOE430A:1431541_AT RIKEN cDNA 1300006C19 gene mm
MOUSE430A_2:1431541_AT RIKEN cDNA 1300006C19 gene mm
MOUSE430_2:1431541_AT RIKEN cDNA 1300006C19 gene mm
MU19KSUBC:TC38773_AT RIKEN cDNA 1300006C19 gene (1300006C19Rik), mRNA mm
YEAST_2:1772876_AT Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; forms a subcomplex with Ost3p and Ost4p and is directly involved in catalysis Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6486 protein amino acid glycosylation inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4576 oligosaccharyl transferase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC24442 Hypothetical protein T12A2.2 [Caenorhabditis elegans] ref|NP_498362.1| T12A2.2 [Caenorhabditis elegans] sp|P46975|STT3_CAEEL Oligosaccharyl transferase STT3 subunit homolog 0.0
blast CAE70740 Hypothetical protein CBG17485 [Caenorhabditis briggsae] 0.0
blast CAG31623 hypothetical protein [Gallus gallus] ref|NP_001026684.1| similar to source of immunodominant MHC-associated peptides; homolog of yeast STT3 [Gallus gallus] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR003674 EMBL-EBI Oligosaccharyl transferase, STT3 subunit 1.0E-126
Pfam IPR003674 EMBL-EBI Oligosaccharyl transferase, STT3 subunit 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_498362.1 12 12-34,82-104,117-136,140-159,172-194,209-231,238-260,270-287,300-322,359-381,388-410,478-500
T12A2.2.3 12 12-34,82-104,117-136,140-159,172-194,209-231,238-260,270-287,300-322,359-381,388-410,478-500
T12A2.2.2 12 12-34,82-104,117-136,140-159,172-194,209-231,238-260,270-287,300-322,359-381,388-410,478-500
T12A2.2.1 12 12-34,82-104,117-136,140-159,172-194,209-231,238-260,270-287,300-322,359-381,388-410,478-500
SNAP00000026127 12 12-34,82-104,117-136,140-159,172-194,209-231,238-260,270-287,300-322,359-381,388-410,478-500
GENEFINDER00000026136 13 12-34,82-104,117-136,140-159,164-186,206-228,240-262,272-289,302-324,361-383,390-407,411-430,505-524
Sequence
Target Sequence 
>C. ELEGANS:187557_S_AT
atcgcaggaatggccaaccgtactacattagttgacaacaatacctggaacaactcgcac
attgcccttgtcggaaaagccatgtcctccaatgaatcagccgcttacgaaattatgacc
gaactcgacgttgactacattcttgttattttcggaggagttattggatattctggagat
gatatcaacaaattcttgtggatggttcgtattgcacaaggagaacacccgaaagatatc
cgcgaggagaactacttcacatcgactggtgaatattccactggagcaggtgcttctgaa
actatgctcaattgcctcatgtacaaaatgagctattatcgttttggtgaaactcgtgtt
ggatacaaccaggctggtggcttcgatagaactcgtggatatgtaattggaaagaaggat
atcactcttgaatacatagaagaagcatatacaacggagaactggcttgtgcgtatctac
aagcgtaagaagcttccaaacagaccaacagtgaagagtgaagaggctaccatcccaatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCGCAGGAATGGCCAACCGTACTA 130 37 1672 Antisense
ATACCTGGAACAACTCGCACATTGC 387 23 1712 Antisense
GTCGGAAAAGCCATGTCCTCCAATG 215 473 1741 Antisense
CCTCCAATGAATCAGCCGCTTACGA 13 267 1757 Antisense
AACTCGACGTTGACTACATTCTTGT 500 141 1793 Antisense
GAACACCCGAAAGATATCCGCGAGG 77 353 1894 Antisense
GAGGAGAACTACTTCACATCGACTG 36 405 1915 Antisense
AACTATGCTCAATTGCCTCATGTAC 328 139 1971 Antisense
CCAGGCTGGTGGCTTCGATAGAACT 24 267 2040 Antisense
GAGAACTGGCTTGTGCGTATCTACA 21 393 2128 Antisense
GAGTGAAGAGGCTACCATCCCAATT 368 401 2187 Antisense
Sequence Source Affymetrix Proprietary Database