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[187554_at]

Description R10E12.2 /REP_DB=WormBase Gene ID /WP=CE00311 /TR=SW:P34553 /GB=CAA82668.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 42      6grp_high1_10_10 > 15      4grp_highest_8_8 > 58      4grp_high_8_8 > 33      6grp_highest_nonorm > 31      6grp_total > 15      4grp_total > 46      6grp_highest_sub > 02      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S2      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 539
1 2 3 4 range
A 1161.6
(3)
P 		922
(0.1)
P 		659.9
(-3)
P 		690
(-2.6)
P 		502
B 1147.9
(3)
P 		834.6
(-0.6)
P 		622.7
(-3)
P 		806.1
(-0.9)
P 		525
C 1095.3
(3)
P 		755.9
(-1.8)
P 		672.5
(-3)
P 		706.9
(-2.5)
P 		423
avr 1134.9
(3) 		837.5
(-0.7) 		651.7
(-3) 		734.3
(-2) 		483

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 606
1 2 3 4 5 6 range
A 1292.9
(3)
P 		1264.7
(2.7)
P 		785.3
(-2)
P 		1027.3
(0.4)
P 		715
(-2.7)
P 		687
(-3)
P 		606
B 1208.7
(2.1)
P 		1293.3
(3)
P 		796.8
(-2)
P 		844.3
(-1.6)
P 		884.3
(-1.2)
P 		702
(-3)
P 		591
C 1237.4
(2.6)
P 		1275.3
(3)
P 		1091.3
(1)
P 		798.5
(-2.3)
P 		739.3
(-2.9)
P 		733.4
(-3)
P 		542
avr 1246.3
(2.7) 		1277.8
(3) 		891.1
(-1.1) 		890
(-1.1) 		779.5
(-2.2) 		707.5
(-3) 		570
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 322
1 2 3 4 range
A 423
(3)
P 		200.1
(-1.2)
P 		172.5
(-1.7)
P 		101
(-3)
P 		322
B 366
(3)
P 		206.1
(-1.3)
P 		144.3
(-3)
P 		171.8
(-2.3)
P 		222
C 362.6
(3)
P 		217.3
(-0.6)
P 		234.4
(-0.2)
P 		119.2
(-3)
P 		243
avr 383.9
(3) 		207.8
(-1.2) 		183.7
(-1.7) 		130.7
(-3) 		253

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 298
1 2 3 4 5 6 range
A 238.3
(-1.5)
P 		449.8
(3)
P 		171
(-2.9)
P 		260.6
(-1)
P 		215.8
(-2)
P 		167.3
(-3)
P 		283
B 420.6
(2.7)
P 		430.5
(3)
P 		193.5
(-3)
P 		223.6
(-2.2)
P 		359.3
(1.2)
P 		261.8
(-1.3)
P 		237
C 458.6
(3)
P 		389.1
(1.6)
P 		277.8
(-0.6)
P 		160.6
(-3)
P 		239.2
(-1.4)
P 		245.5
(-1.3)
P 		298
avr 372.5
(1.5) 		423.1
(3) 		214.1
(-3) 		214.9
(-3) 		271.4
(-1.4) 		224.9
(-2.7) 		209
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187554_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6748
Sequence Type Exemplar sequence
Representative Public ID R10E12.2 NCBI
Target Description R10E12.2 /REP_DB=WormBase Gene ID /WP=CE00311 /TR=SW:P34553 /GB=CAA82668.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066813(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066813 NCBI Caenorhabditis elegans R10E12.2 (R10E12.2) mRNA, complete cds. 11/11 None
SNAP00000019729 ENSEMBL cdna:SNAP chromosome:CEL140:III:9963220:9966583:-1 11/11 None
GENEFINDER00000019730 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:9963220:9966583:-1 11/11 None
R10E12.2 ENSEMBL cdna:known chromosome:CEL140:III:9963050:9973756:-1 gene:R10E12.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:9963215-9966579(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl R10E12.2
Entrez Gene 187782 Entrez gene
SwissProt P34553 EMBL-EBI
WormBase CE00311 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA82668 Hypothetical protein R10E12.2 [Caenorhabditis elegans] ref|NP_499214.1| R10E12.2 [Caenorhabditis elegans] sp|P34553|YNK2_CAEEL Hypothetical protein R10E12.2 in chromosome III 0.0
blast CAE65077 Hypothetical protein CBG09935 [Caenorhabditis briggsae] 1.0E-173
blast CAA83591 Hypothetical protein C05B5.4 [Caenorhabditis elegans] ref|NP_499216.1| C05B5.4 [Caenorhabditis elegans] sp|P34292|YKO4_CAEEL Hypothetical protein C05B5.4 in chromosome III 2.0E-61
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_499214.1 1 214-236
R10E12.2 1 214-236
SNAP00000019729 1 214-236
GENEFINDER00000019730 1 214-236
Sequence
Target Sequence 
>C. ELEGANS:187554_AT
cgtggctttttggatcatcatcttcattgatttctgatgatcgaattgttcatattggag
ttcttgtcctggcaattgttgctgtcttgtcttcaactgcatttgctatcattttggtta
tgtatttgagaaataagagattggtcaaggagacactcaagaagccacgttttcatccgt
atatctcggtgaaggggcatgaaattgctgaggacaattgcagatatgatcttccatggg
aacagcaacaacgccccctaacctactggatgacgtcatcaaacaaatcatccgaatcca
caatgacgtcaccattggattcagcttcatcccttcatggaagtggtggaagtcatcaga
atccacacatgtacaattctcatcatcatcagcatcagcaacagcagaatgctcatccat
cagaaatgtatcattcaacatatacacgtgatggatatcaaacatatcgtccaccaccac
catcagttcatccaccaatatttccaccacaaacacaattattccatccaccaacatact
caacacaacgtcacgtaacatctccaaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGTGGCTTTTTGGATCATCATCTTC 437 245 593 Antisense
TTGGAGTTCTTGTCCTGGCAATTGT 403 707 647 Antisense
GTTGCTGTCTTGTCTTCAACTGCAT 70 441 670 Antisense
GGAGACACTCAAGAAGCCACGTTTT 654 517 741 Antisense
GCCACGTTTTCATCCGTATATCTCG 184 279 756 Antisense
CCCCTAACCTACTGGATGACGTCAT 23 257 847 Antisense
CATCCGAATCCACAATGACGTCACC 646 211 881 Antisense
GACGTCACCATTGGATTCAGCTTCA 67 379 897 Antisense
TTCAGCTTCATCCCTTCATGGAAGT 304 687 912 Antisense
ACCATCAGTTCATCCACCAATATTT 135 87 1071 Antisense
ACAACGTCACGTAACATCTCCAAAT 162 113 1137 Antisense
Sequence Source Affymetrix Proprietary Database