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[187551_at]

Description F09E5.8 /REP_DB=WormBase Gene ID /WP=CE02615 /TR=SW:P52057 /GB=AAA79348.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 45      6grp_high1_10_10 > 54      4grp_highest_8_8 > 03      4grp_high_8_8 > 38      6grp_highest_nonorm > 76      6grp_total > 55      4grp_total > 41      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 506
1 2 3 4 range
A 897.4
(-3)
P 		1372.1
(3)
P 		1235.1
(1.3)
P 		1217.6
(1)
P 		475
B 944.8
(-3)
P 		1341
(3)
P 		1340.6
(3)
P 		1292.7
(2.3)
P 		396
C 1025.1
(-3)
P 		1350.9
(2.2)
P 		1402.9
(3)
P 		1092.7
(-1.9)
P 		378
avr 955.8
(-3) 		1354.7
(3) 		1326.2
(2.6) 		1201
(0.7) 		399

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 789
1 2 3 4 5 6 range
A 925.6
(-2.6)
P 		1164.6
(-0.8)
P 		1667
(3)
P 		1118.4
(-1.2)
P 		877.8
(-3)
P 		1012.7
(-2)
P 		789
B 1187.9
(-0.6)
P 		1374.7
(1.5)
P 		1513.8
(3)
P 		1167.7
(-0.8)
P 		964.5
(-3)
P 		1298.1
(0.6)
P 		549
C 986.5
(-2.1)
P 		1510.2
(3)
P 		1104.9
(-0.9)
P 		1334.3
(1.3)
P 		893.2
(-3)
P 		1413.1
(2.1)
P 		617
avr 1033.3
(-1.6) 		1349.8
(2.1) 		1428.6
(3) 		1206.8
(0.4) 		911.8
(-3) 		1241.3
(0.8) 		517
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 311
1 2 3 4 range
A 326.8
(3)
P 		297.9
(1.8)
P 		322.9
(2.8)
P 		178.2
(-3)
P 		149
B 301.3
(-0.2)
P 		331.2
(3)
P 		310.7
(0.8)
P 		275.5
(-3)
P 		56
C 339.3
(0.1)
P 		388.4
(1)
P 		488.9
(3)
P 		184.2
(-3)
P 		305
avr 322.5
(1.1) 		339.2
(1.7) 		374.2
(3) 		212.6
(-3) 		162

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 314
1 2 3 4 5 6 range
A 170.6
(-3)
P 		414.2
(3)
P 		363
(1.7)
P 		283.6
(-0.2)
P 		264.9
(-0.7)
P 		246.6
(-1.1)
P 		244
B 413.3
(0.6)
P 		457.6
(2.1)
P 		367.6
(-1)
P 		309.3
(-3)
P 		391.9
(-0.2)
P 		484.1
(3)
P 		175
C 365.6
(-0.1)
P 		460.8
(2.6)
P 		281.2
(-2.6)
P 		268.3
(-3)
P 		289
(-2.4)
P 		473
(3)
P 		205
avr 316.5
(-1.9) 		444.2
(3) 		337.3
(-1.1) 		287.1
(-3) 		315.3
(-1.9) 		401.2
(1.4) 		157
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187551_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5618
Sequence Type Exemplar sequence
Representative Public ID F09E5.8 NCBI
Target Description F09E5.8 /REP_DB=WormBase Gene ID /WP=CE02615 /TR=SW:P52057 /GB=AAA79348.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062600(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062600 NCBI Caenorhabditis elegans F09E5.8 (F09E5.8) mRNA, complete cds. 11/11 None
SNAP00000044010 ENSEMBL cdna:SNAP chromosome:CEL140:II:5350646:5351904:1 11/11 None
GENEFINDER00000044033 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5350646:5351904:1 11/11 None
F09E5.8 ENSEMBL cdna:known chromosome:CEL140:II:5350639:5351921:1 gene:F09E5.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5350643-5351902(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F09E5.8
Entrez Gene 173906 Entrez gene
SwissProt P52057 EMBL-EBI
WormBase CE02615 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:264349_AT alanine racemase family protein at
CANINE_2:CFAAFFX.10271.1.S1_AT similar to Proline synthetase co-transcribed bacterial homolog protein cfa
CANINE_2:CFAAFFX.10272.1.S1_S_AT similar to Proline synthetase co-transcribed bacterial homolog protein cfa
DROSOPHILA_2:1638630_AT dm
DROSGENOME1:150876_AT dm
CHICKEN:GGA.1013.1.S1_AT similar to Proline synthetase co-transcribed bacterial homolog protein gga
CHICKEN:GGA.1013.2.S1_A_AT similar to Proline synthetase co-transcribed bacterial homolog protein gga
CHICKEN:GGA.1952.1.S1_AT similar to Proline synthetase co-transcribed bacterial homolog protein gga
HG-U133A:209384_AT proline synthetase co-transcribed homolog (bacterial) hs
HG-U133A_2:209384_AT proline synthetase co-transcribed homolog (bacterial) hs
HG-FOCUS:209384_AT proline synthetase co-transcribed homolog (bacterial) hs
HG-U133_PLUS_2:209384_AT proline synthetase co-transcribed homolog (bacterial) hs
HG-U133A:209385_S_AT proline synthetase co-transcribed homolog (bacterial) hs
HG-U133_PLUS_2:209385_S_AT proline synthetase co-transcribed homolog (bacterial) hs
HG-U133A_2:209385_S_AT proline synthetase co-transcribed homolog (bacterial) hs
HG-U95AV2:40424_AT proline synthetase co-transcribed homolog (bacterial) hs
HG-U95AV2:40545_AT proline synthetase co-transcribed homolog (bacterial) hs
HU35KSUBA:AA431066_AT proline synthetase co-transcribed homolog (bacterial) hs
HU35KSUBC:RC_AA129921_AT proline synthetase co-transcribed homolog (bacterial) hs
HU35KSUBD:RC_H93562_AT proline synthetase co-transcribed homolog (bacterial) hs
U133_X3P:G12052757_3P_A_AT proline synthetase co-transcribed homolog (bacterial) hs
U133_X3P:HS.301959.2.S1_3P_A_AT proline synthetase co-transcribed homolog (bacterial) hs
U133_X3P:HS.301959.0.A2_3P_AT proline synthetase co-transcribed homolog (bacterial) hs
U133_X3P:209385_3P_S_AT proline synthetase co-transcribed homolog (bacterial) hs
HG-U133A_2:214545_S_AT proline synthetase co-transcribed homolog (bacterial) hs
HG-U133_PLUS_2:214545_S_AT proline synthetase co-transcribed homolog (bacterial) hs
HG-U133A:214545_S_AT proline synthetase co-transcribed homolog (bacterial) hs
HG-U95E:80540_R_AT proline synthetase co-transcribed homolog (bacterial) hs
HG-U133_PLUS_2:216519_S_AT Proline synthetase co-transcribed homolog (bacterial) hs
HG-U133A_2:216519_S_AT Proline synthetase co-transcribed homolog (bacterial) hs
HG-U133A:216519_S_AT Proline synthetase co-transcribed homolog (bacterial) hs
HG-U133_PLUS_2:227315_X_AT Proline synthetase co-transcribed homolog (bacterial) hs
HG-U133B:227315_X_AT Proline synthetase co-transcribed homolog (bacterial) hs
HG-U95D:91148_R_AT Proline synthetase co-transcribed homolog (bacterial) hs
U133_X3P:HS.301959.1.A1_3P_A_AT Proline synthetase co-transcribed homolog (bacterial) hs
MG-U74AV2:101568_AT proline synthetase co-transcribed mm
MOE430A:1423044_AT proline synthetase co-transcribed mm
MOUSE430A_2:1423044_AT proline synthetase co-transcribed mm
MOUSE430_2:1423044_AT proline synthetase co-transcribed mm
MOE430B:1441685_AT proline synthetase co-transcribed mm
MOUSE430_2:1441685_AT proline synthetase co-transcribed mm
MG-U74AV2:101567_AT Proline synthetase co-transcribed (Prosc), mRNA mm
MU11KSUBA:AA537449_AT Proline synthetase co-transcribed (Prosc), mRNA mm
MU19KSUBA:TC16487_AT Proline synthetase co-transcribed (Prosc), mRNA mm
MU19KSUBC:TC40046_AT Proline synthetase co-transcribed (Prosc), mRNA mm
RG-U34C:RC_AI177374_AT proline synthetase co-transcribed (predicted) rn
RAE230A:1389549_AT proline synthetase co-transcribed (predicted) rn
RAT230_2:1389549_AT proline synthetase co-transcribed (predicted) rn
RAE230B:1383722_AT proline synthetase co-transcribed (predicted) rn
RAT230_2:1383722_AT proline synthetase co-transcribed (predicted) rn
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
8784 alanine racemase activity inferred from electronic annotation QuickGO AmiGO
30170 pyridoxal phosphate binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA79348 Hypothetical protein F09E5.8 [Caenorhabditis elegans] ref|NP_495001.1| F09E5.8 [Caenorhabditis elegans] sp|P52057|YU68_CAEEL Hypothetical UPF0001 protein F09E5.8 in chromosome II 1.0E-138
blast CAE59082 Hypothetical protein CBG02374 [Caenorhabditis briggsae] 1.0E-110
blast AAF57017 CG1983-PA [Drosophila melanogaster] ref|NP_651776.2| CG1983-PA [Drosophila melanogaster] gb|AAO39501.1| RE46560p [Drosophila melanogaster] 2.0E-51
Protein Domains
Database ID Description E-Value
Pfam IPR001608 EMBL-EBI Protein of unknown function UPF0001 4.9E-10
Sequence
Target Sequence 
>C. ELEGANS:187551_AT
atattcgttggcattttatcggacaagtgcaatcaaataaaattggaaaaatttgcaatt
ctcctggactttggtgtgttgaaacagtggagactgagaaacatgctcggattttcgaca
aggaatggtcaaaacacggagccaatttgtcaccactacgagttctcgtgcaagtaaata
catccggagaagataataaaggaggaatcgagattggagaagcaccgaaacttgcggaat
tcattcgaaaagaatgccaaaacttgaaatttgatggattcatgactatcggatcgtttg
acaacagccacgcaagcggagaaaatccggatttcgagaagctcttcaaagtccgacaaa
catgggcagaacaaacaggtgaatctgcggattcagtagaactctcaatgggaatgtccg
atgatttccttcaagc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATATTCGTTGGCATTTTATCGGACA 43 15 239 Antisense
AATTCTCCTGGACTTTGGTGTGTTG 401 177 295 Antisense
GAAACATGCTCGGATTTTCGACAAG 98 357 336 Antisense
AAACACGGAGCCAATTTGTCACCAC 392 129 370 Antisense
TTGTCACCACTACGAGTTCTCGTGC 685 703 385 Antisense
GCACCGAAACTTGCGGAATTCATTC 426 295 460 Antisense
GATGGATTCATGACTATCGGATCGT 470 407 511 Antisense
CGGATCGTTTGACAACAGCCACGCA 660 243 528 Antisense
GAGAAGCTCTTCAAAGTCCGACAAA 18 393 574 Antisense
GTAGAACTCTCAATGGGAATGTCCG 477 455 634 Antisense
GAATGTCCGATGATTTCCTTCAAGC 338 331 650 Antisense
Sequence Source Affymetrix Proprietary Database