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[187528_s_at]

Description Y71F9AL.14 /REP_DB=WormBase Gene ID /WP=CE27022 /TR=Q9N4I1 /GB=AAF36009.2 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 67      6grp_high1_10_10 > 85      4grp_highest_8_8 > 63      4grp_high_8_8 > 28      6grp_highest_nonorm > 85      6grp_total > 66      4grp_total > 28      6grp_highest_sub > In      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 345
1 2 3 4 range
A 662.9
(-3)
P 		934.9
(3)
P 		689.1
(-2.4)
P 		910.2
(2.5)
P 		272
B 770.8
(3)
P 		718.9
(0.5)
P 		660
(-2.3)
P 		644.3
(-3)
P 		127
C 716.6
(2)
P 		599.4
(-2.6)
P 		589.8
(-3)
P 		741
(3)
P 		151
avr 716.8
(0.6) 		751.1
(2.3) 		646.3
(-3) 		765.2
(3) 		119

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 526
1 2 3 4 5 6 range
A 643
(-3)
P 		722.3
(-1.1)
P 		894.1
(3)
P 		732.2
(-0.9)
P 		663.3
(-2.5)
P 		711.4
(-1.4)
P 		251
B 623.4
(-2.7)
P 		609.9
(-3)
P 		761.4
(-0.4)
P 		608.2
(-3)
P 		875.8
(1.5)
P 		965.6
(3)
P 		357
C 615.8
(-2.7)
P 		636.4
(-2.5)
P 		700.9
(-1.7)
P 		644.2
(-2.4)
P 		588.5
(-3)
P 		1114.3
(3)
P 		526
avr 627.4
(-3) 		656.2
(-2.4) 		785.5
(0.1) 		661.5
(-2.3) 		709.2
(-1.4) 		930.4
(3) 		303
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 121
1 2 3 4 range
A 241.4
(3)
P 		202.9
(0.9)
P 		180.2
(-0.4)
P 		133.2
(-3)
P 		108
B 245.8
(3)
P 		177.5
(-0.8)
P 		152.9
(-2.1)
P 		137.3
(-3)
P 		109
C 237.2
(3)
P 		172.3
(-0.5)
P 		205.6
(1.3)
P 		124.9
(-3)
P 		112
avr 241.5
(3) 		184.2
(-0.1) 		179.6
(-0.4) 		131.8
(-3) 		110

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 255
1 2 3 4 5 6 range
A 118.5
(-3)
P 		256.9
(3)
P 		194.7
(0.3)
P 		185.7
(-0.1)
P 		200.1
(0.5)
P 		173.2
(-0.6)
P 		138
B 216.9
(-1.3)
P 		203
(-1.7)
P 		184.9
(-2.3)
P 		161.1
(-3)
P 		355.8
(2.9)
P 		360.1
(3)
P 		199
C 228.2
(-0.6)
P 		194.2
(-1.4)
P 		178.4
(-1.8)
P 		129.5
(-3)
P 		190.4
(-1.5)
P 		373
(3)
P 		244
avr 187.9
(-1.8) 		218
(-0.5) 		186
(-1.9) 		158.8
(-3) 		248.8
(0.8) 		302.1
(3) 		143
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 187528_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.291
Sequence Type Exemplar sequence
Representative Public ID Y71F9AL.14 NCBI
Target Description Y71F9AL.14 /REP_DB=WormBase Gene ID /WP=CE27022 /TR=Q9N4I1 /GB=AAF36009.2 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058664(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058664 NCBI Caenorhabditis elegans Y71F9AL.14 (Y71F9AL.14) mRNA, complete cds. 11/11 A 
SNAP00000005888 ENSEMBL cdna:SNAP chromosome:CEL140:I:2880922:2883441:-1 11/11 None
GENEFINDER00000005904 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:2881288:2883441:-1 11/11 None
Y71F9AL.14 ENSEMBL cdna:known chromosome:CEL140:I:2880920:2883455:-1 gene:Y71F9AL.14 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:2881892-2883441(-) 96.63 100.0
Public Domain and Genome References
Gene Title
Ensembl Y71F9AL.14
Entrez Gene 171856 Entrez gene
SwissProt Q9N4I1 EMBL-EBI
WormBase CE27022 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.27035.1.S1_AT similar to Dynactin subunit 5 (Dynactin subunit p25) cfa
CANINE_2:CFAAFFX.27035.1.S1_S_AT similar to Dynactin subunit 5 (Dynactin subunit p25) cfa
DROSOPHILA_2:1623231_AT dynactin-subunit-p25 dm
DROSGENOME1:144314_AT dynactin-subunit-p25 dm
CHICKEN:GGA.9600.1.S1_AT dynactin 5 (p25) gga
CHICKEN:GGA.9600.2.S1_AT dynactin 5 (p25) gga
CHICKEN:GGA.9600.3.S1_A_AT dynactin 5 (p25) gga
HG-U133_PLUS_2:209232_S_AT dynactin 5 (p25) hs
HG-U133A_2:209232_S_AT dynactin 5 (p25) hs
HG-U133A:209232_S_AT dynactin 5 (p25) hs
HU35KSUBA:RC_AA621434_AT dynactin 5 (p25) hs
HU35KSUBB:RC_AA004509_AT dynactin 5 (p25) hs
HU35KSUBD:RC_AA280837_AT dynactin 5 (p25) hs
U133_X3P:G13278857_3P_A_AT dynactin 5 (p25) hs
U133_X3P:HS.111429.0.S1_3P_A_AT dynactin 5 (p25) hs
HG-U133A_2:209231_S_AT dynactin 5 (p25) hs
HG-U133_PLUS_2:209231_S_AT dynactin 5 (p25) hs
HG-U133A:209231_S_AT dynactin 5 (p25) hs
HG-U95AV2:37434_AT dynactin 5 (p25) hs
HG-U95B:43113_AT dynactin 5 (p25) hs
HG-U95B:43114_G_AT dynactin 5 (p25) hs
HG-U95C:65610_AT dynactin 5 (p25) hs
HG-U95C:66137_AT Dynactin 5 (p25) hs
MOE430B:1415748_A_AT dynactin 5 mm
MOE430A:1415748_A_AT dynactin 5 mm
MOUSE430A_2:1415748_A_AT dynactin 5 mm
MOUSE430_2:1415748_A_AT dynactin 5 mm
MU11KSUBA:AA110732_S_AT dynactin 5 mm
MG-U74AV2:96636_AT dynactin 5 mm
MOE430B:1458247_S_AT dynactin 5 mm
MOUSE430_2:1458247_S_AT dynactin 5 mm
MG-U74CV2:167183_AT Dynactin 5, mRNA (cDNA clone MGC:19347 IMAGE:4235312) mm
MU19KSUBA:TC22936_AT Dynactin 5, mRNA (cDNA clone MGC:19347 IMAGE:4235312) mm
RAE230A:1373441_AT Similar to dynactin subunit p25 (predicted) rn
RAT230_2:1373441_AT Similar to dynactin subunit p25 (predicted) rn
Protein Similarities
Method ID Description E-Value
blast AAF36009 Hypothetical protein Y71F9AL.14 [Caenorhabditis elegans] ref|NP_491065.1| Y71F9AL.14 [Caenorhabditis elegans] 1.0E-104
blast CAE60589 Hypothetical protein CBG04225 [Caenorhabditis briggsae] 1.0E-91
blast AAF36009 Hypothetical protein Y71F9AL.14 [Caenorhabditis elegans] ref|NP_491065.1| Y71F9AL.14 [Caenorhabditis elegans] 6.0E-84
blast CAE60589 Hypothetical protein CBG04225 [Caenorhabditis briggsae] 6.0E-76
blast AAH87561 Dynactin 4 [Xenopus tropicalis] ref|NP_001011198.1| dynactin 4 [Xenopus tropicalis] 9.0E-43
Protein Domains
Database ID Description E-Value
Pfam IPR001451 EMBL-EBI Bacterial transferase hexapeptide repeat 1.5
Pfam IPR001451 EMBL-EBI Bacterial transferase hexapeptide repeat 1.0
Pfam IPR001451 EMBL-EBI Bacterial transferase hexapeptide repeat 0.23
Pfam IPR001451 EMBL-EBI Bacterial transferase hexapeptide repeat 0.022
Pfam IPR001451 EMBL-EBI Bacterial transferase hexapeptide repeat 5.0
Sequence
Target Sequence 
>C. ELEGANS:187528_S_AT
aagaatgtgtcgtcaatgccgctcaaatctacgcatttgtacatcttggagctcgtgcgg
tgctcggaaatggttgtgtgattcgagaatgttcgagggttcttccggatactgtagttc
cagcggacgcactttttccaccgtactcaacgatcggcgggaatcctg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGAATGTGTCGTCAATGCCGCTCA 565 151 296 Antisense
TGTCGTCAATGCCGCTCAAATCTAC 107 559 303 Antisense
ATGCCGCTCAAATCTACGCATTTGT 329 41 311 Antisense
ACGCATTTGTACATCTTGGAGCTCG 3 93 326 Antisense
ACATCTTGGAGCTCGTGCGGTGCTC 328 105 336 Antisense
TGCGGTGCTCGGAAATGGTTGTGTG 164 583 351 Antisense
GATTCGAGAATGTTCGAGGGTTCTT 300 433 375 Antisense
GGATACTGTAGTTCCAGCGGACGCA 214 509 402 Antisense
TGTAGTTCCAGCGGACGCACTTTTT 58 561 408 Antisense
TTTTCCACCGTACTCAACGATCGGC 501 673 429 Antisense
TACTCAACGATCGGCGGGAATCCTG 579 643 439 Antisense
Sequence Source Affymetrix Proprietary Database