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[187527_at]

Description Y71F9AL.14 /REP_DB=WormBase Gene ID /WP=CE27022 /TR=Q9N4I1 /GB=AAF36009.2 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 96      6grp_high1_10_10 > 79      4grp_highest_8_8 > 54      4grp_high_8_8 > 10      6grp_highest_nonorm > 82      6grp_total > 79      4grp_total > 22      6grp_highest_sub > Cu      6grp_high1_sub > Cu      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 246
1 2 3 4 range
A 324.6
(-0.6)
P 		384
(3)
P 		285.9
(-3)
P 		344.9
(0.6)
P 		98
B 509.1
(3)
P 		349.8
(-2.4)
P 		331.2
(-3)
P 		478.1
(2)
P 		178
C 493.6
(1.7)
P 		358.5
(-3)
P 		431
(-0.5)
P 		532.2
(3)
P 		174
avr 442.4
(2.5) 		364.1
(-2.1) 		349.4
(-3) 		451.7
(3) 		102

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 405
1 2 3 4 5 6 range
A 405.3
(1.3)
P 		388.9
(0.6)
P 		444.2
(3)
P 		351.3
(-1)
P 		306.3
(-3)
P 		311
(-2.8)
P 		138
B 252.8
(-3)
P 		393.1
(0.4)
P 		428.2
(1.2)
P 		365.4
(-0.3)
P 		502
(3)
P 		485.7
(2.6)
P 		249
C 515.6
(0.4)
P 		390
(-1.9)
P 		331.7
(-3)
P 		352.9
(-2.6)
P 		455.9
(-0.7)
P 		658.1
(3)
P 		326
avr 391.2
(-1.4) 		390.7
(-1.4) 		401.4
(-0.9) 		356.5
(-3) 		421.4
(0) 		484.9
(3) 		128
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 113
1 2 3 4 range
A 118.2
(3)
P 		83.3
(-0.1)
P 		74.7
(-0.9)
P 		50.5
(-3)
P 		68
B 162.4
(3)
P 		86.4
(-2.3)
P 		76.7
(-3)
P 		101.9
(-1.2)
P 		86
C 163.4
(3)
P 		103.1
(-1.9)
P 		150.2
(1.9)
P 		89.7
(-3)
P 		74
avr 148
(3) 		90.9
(-2.1) 		100.5
(-1.2) 		80.7
(-3) 		67

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 149
1 2 3 4 5 6 range
A 74.7
(-3)
P 		138.3
(3)
P 		96.7
(-0.9)
P 		89.1
(-1.6)
P 		92.4
(-1.3)
P 		75.7
(-2.9)
P 		64
B 88
(-3)
P 		130.8
(-0.8)
P 		104
(-2.2)
P 		96.8
(-2.5)
P 		203.9
(3)
P 		181.1
(1.8)
P 		116
C 191.1
(1.8)
P 		119
(-1.1)
P 		84.4
(-2.5)
P 		71
(-3)
P 		147.5
(0.1)
P 		220.3
(3)
P 		149
avr 117.9
(-0.4) 		129.4
(0.6) 		95
(-2.2) 		85.6
(-3) 		147.9
(2.1) 		159
(3) 		73
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187527_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.291
Sequence Type Exemplar sequence
Representative Public ID Y71F9AL.14 NCBI
Target Description Y71F9AL.14 /REP_DB=WormBase Gene ID /WP=CE27022 /TR=Q9N4I1 /GB=AAF36009.2 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058664(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058664 NCBI Caenorhabditis elegans Y71F9AL.14 (Y71F9AL.14) mRNA, complete cds. 10/11 A 
SNAP00000005888 ENSEMBL cdna:SNAP chromosome:CEL140:I:2880922:2883441:-1 10/11 None
GENEFINDER00000005904 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:2881288:2883441:-1 10/11 None
Y71F9AL.14 ENSEMBL cdna:known chromosome:CEL140:I:2880920:2883455:-1 gene:Y71F9AL.14 10/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:2881892-2883441(-) 96.63 100.0
Public Domain and Genome References
Gene Title
Ensembl Y71F9AL.14
Entrez Gene 171856 Entrez gene
SwissProt Q9N4I1 EMBL-EBI
WormBase CE27022 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.27035.1.S1_AT similar to Dynactin subunit 5 (Dynactin subunit p25) cfa
CANINE_2:CFAAFFX.27035.1.S1_S_AT similar to Dynactin subunit 5 (Dynactin subunit p25) cfa
DROSOPHILA_2:1623231_AT dynactin-subunit-p25 dm
DROSGENOME1:144314_AT dynactin-subunit-p25 dm
CHICKEN:GGA.9600.1.S1_AT dynactin 5 (p25) gga
CHICKEN:GGA.9600.2.S1_AT dynactin 5 (p25) gga
CHICKEN:GGA.9600.3.S1_A_AT dynactin 5 (p25) gga
HG-U133_PLUS_2:209232_S_AT dynactin 5 (p25) hs
HG-U133A_2:209232_S_AT dynactin 5 (p25) hs
HG-U133A:209232_S_AT dynactin 5 (p25) hs
HU35KSUBA:RC_AA621434_AT dynactin 5 (p25) hs
HU35KSUBB:RC_AA004509_AT dynactin 5 (p25) hs
HU35KSUBD:RC_AA280837_AT dynactin 5 (p25) hs
U133_X3P:G13278857_3P_A_AT dynactin 5 (p25) hs
U133_X3P:HS.111429.0.S1_3P_A_AT dynactin 5 (p25) hs
HG-U133A_2:209231_S_AT dynactin 5 (p25) hs
HG-U133_PLUS_2:209231_S_AT dynactin 5 (p25) hs
HG-U133A:209231_S_AT dynactin 5 (p25) hs
HG-U95AV2:37434_AT dynactin 5 (p25) hs
HG-U95B:43113_AT dynactin 5 (p25) hs
HG-U95B:43114_G_AT dynactin 5 (p25) hs
HG-U95C:65610_AT dynactin 5 (p25) hs
HG-U95C:66137_AT Dynactin 5 (p25) hs
MOE430B:1415748_A_AT dynactin 5 mm
MOE430A:1415748_A_AT dynactin 5 mm
MOUSE430A_2:1415748_A_AT dynactin 5 mm
MOUSE430_2:1415748_A_AT dynactin 5 mm
MU11KSUBA:AA110732_S_AT dynactin 5 mm
MG-U74AV2:96636_AT dynactin 5 mm
MOE430B:1458247_S_AT dynactin 5 mm
MOUSE430_2:1458247_S_AT dynactin 5 mm
MG-U74CV2:167183_AT Dynactin 5, mRNA (cDNA clone MGC:19347 IMAGE:4235312) mm
MU19KSUBA:TC22936_AT Dynactin 5, mRNA (cDNA clone MGC:19347 IMAGE:4235312) mm
RAE230A:1373441_AT Similar to dynactin subunit p25 (predicted) rn
RAT230_2:1373441_AT Similar to dynactin subunit p25 (predicted) rn
Protein Similarities
Method ID Description E-Value
blast AAF36009 Hypothetical protein Y71F9AL.14 [Caenorhabditis elegans] ref|NP_491065.1| Y71F9AL.14 [Caenorhabditis elegans] 1.0E-104
blast CAE60589 Hypothetical protein CBG04225 [Caenorhabditis briggsae] 1.0E-91
blast AAF36009 Hypothetical protein Y71F9AL.14 [Caenorhabditis elegans] ref|NP_491065.1| Y71F9AL.14 [Caenorhabditis elegans] 6.0E-84
blast CAE60589 Hypothetical protein CBG04225 [Caenorhabditis briggsae] 6.0E-76
blast AAH87561 Dynactin 4 [Xenopus tropicalis] ref|NP_001011198.1| dynactin 4 [Xenopus tropicalis] 9.0E-43
Protein Domains
Database ID Description E-Value
Pfam IPR001451 EMBL-EBI Bacterial transferase hexapeptide repeat 1.5
Pfam IPR001451 EMBL-EBI Bacterial transferase hexapeptide repeat 1.0
Pfam IPR001451 EMBL-EBI Bacterial transferase hexapeptide repeat 0.23
Pfam IPR001451 EMBL-EBI Bacterial transferase hexapeptide repeat 0.022
Pfam IPR001451 EMBL-EBI Bacterial transferase hexapeptide repeat 5.0
Sequence
Target Sequence 
>C. ELEGANS:187527_AT
atggatcttccaattgtctactacgatgagacggaatgggcgaagacgcatacgggaaat
aaagtgaataagaagcatgcaattgctggaacacagaatattttaattgctggaaaaaca
ataatcgaggaaggagtgacaattcgcggtgatctggcgacagttaaaattggaaaatat
tgtgttctaaagtctcgatgtaacatccgtccatgcatgaaaatattcagcaaaaaacca
acaatgtgcaacgttatgattggtgattatgtatttattgaagaagaatgtgtcgtcaat
gccgctcaaatctacgcatttgtacatcttggagctcgtgcggtgctcggaaatggttgt
gtgattcgagaatgttcgagggttcttccggatactgtagttccagcggacgcacttttt
ccaccgtactcaacgatcggcgggaatcctgagccggaaaatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGGATCTTCCAATTGTCTACTACG 602 51 13 Antisense
TCTTCCAATTGTCTACTACGATGAG 510 617 18 Antisense
GATGAGACGGAATGGGCGAAGACGC 460 411 37 Antisense
GGGCGAAGACGCATACGGGAAATAA 220 487 50 Antisense
AGGAGTGACAATTCGCGGTGATCTG 549 55 144 Antisense
AATTCGCGGTGATCTGGCGACAGTT 26 177 153 Antisense
ATATTGTGTTCTAAAGTCTCGATGT 254 19 189 Antisense
GTCTCGATGTAACATCCGTCCATGC 365 467 204 Antisense
GTAACATCCGTCCATGCATGAAAAT 383 461 212 Antisense
AAACCAACAATGTGCAACGTTATGA 378 125 247 Antisense
GGCGGGAATCCTGAGCCGGAAAATC 421 531 451 Antisense
Sequence Source Affymetrix Proprietary Database