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[187523_s_at]

Description H06H21.10 /REP_DB=WormBase Gene ID /WP=CE27180 /GB=AAK29849.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=ATPase
Cluster 6grp_highest_10_10 > 60      6grp_high1_10_10 > 17      4grp_highest_8_8 > 53      4grp_high_8_8 > 09      6grp_highest_nonorm > 70      6grp_total > 06      4grp_total > 30      6grp_highest_sub > De      6grp_cgc > 13      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 427
1 2 3 4 range
A 520.4
(3)
P 		183.5
(-2.4)
P 		146.6
(-3)
P 		335.8
(0)
P 		374
B 353.5
(3)
P 		177.5
(-1.1)
P 		93.4
(-3)
P 		350
(2.9)
P 		260
C 399.8
(3)
P 		185.6
(-1.8)
P 		134.4
(-3)
P 		376.8
(2.5)
P 		265
avr 424.6
(3) 		182.2
(-1.9) 		124.8
(-3) 		354.2
(1.6) 		300

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 315
1 2 3 4 5 6 range
A 323
(2.1)
P 		344.7
(3)
P 		213.9
(-2.2)
P 		254.4
(-0.6)
P 		192.5
(-3)
P 		198.3
(-2.8)
P 		152
B 495.7
(3)
P 		285.1
(-1)
P 		234.3
(-2)
P 		191.2
(-2.8)
P 		191.3
(-2.8)
P 		181.2
(-3)
P 		315
C 438.5
(3)
P 		286.4
(-1.2)
P 		229.3
(-2.7)
P 		245.8
(-2.3)
P 		227.1
(-2.8)
P 		218.6
(-3)
P 		220
avr 419.1
(3) 		305.4
(-0.1) 		225.8
(-2.3) 		230.5
(-2.2) 		203.6
(-2.9) 		199.4
(-3) 		220
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 168
1 2 3 4 range
A 189.5
(3)
P 		39.8
(-2.9)
P 		38.3
(-3)
P 		49.2
(-2.6)
P 		151
B 112.7
(3)
P 		43.8
(-1.5)
P 		21.6
(-3)
P 		74.6
(0.5)
P 		91
C 132.3
(3)
P 		53.4
(-2.5)
P 		46.8
(-3)
P 		63.5
(-1.8)
P 		86
avr 144.8
(3) 		45.7
(-2.4) 		35.6
(-3) 		62.4
(-1.5) 		109

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 126
1 2 3 4 5 6 range
A 59.5
(-2)
P 		122.6
(3)
P 		46.6
(-3)
P 		64.5
(-1.6)
P 		58.1
(-2.1)
P 		48.3
(-2.9)
P 		76
B 172.5
(3)
P 		94.9
(-0.8)
P 		56.9
(-2.7)
P 		50.6
(-3)
P 		77.7
(-1.7)
P 		67.6
(-2.2)
P 		122
C 162.5
(3)
P 		87.4
(-1)
P 		58.4
(-2.5)
P 		49.4
(-3)
P 		73.5
(-1.7)
P 		73.2
(-1.7)
P 		113
avr 131.5
(3) 		101.6
(0.7) 		54
(-3) 		54.8
(-2.9) 		69.8
(-1.8) 		63
(-2.3) 		78
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 187523_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10727
Sequence Type Exemplar sequence
Representative Public ID H06H21.10 NCBI
Target Description H06H21.10 /REP_DB=WormBase Gene ID /WP=CE27180 /GB=AAK29849.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=ATPase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028082(11), NM_001028081(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028082 NCBI Caenorhabditis elegans H06H21.10b (ATPase) mRNA, complete cds. 11/11 None
NM_001028081 NCBI Caenorhabditis elegans H06H21.10a (ATPase) mRNA, complete cds. 11/11 None
H06H21.10b ENSEMBL cdna:known chromosome:CEL140:IV:4827862:4839077:-1 gene:H06H21.10 11/11 None
H06H21.10a ENSEMBL cdna:known chromosome:CEL140:IV:4828066:4841117:-1 gene:H06H21.10 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:4828071-4834803(-) 100.0 100.0
Public Domain and Genome References
Ensembl H06H21.10
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK29849 Transbilayer amphipath transporters (subfamily iv p-type atpase) protein 2, isoform a [Caenorhabditis elegans] ref|NP_001023252.1| H06H21.10a [Caenorhabditis elegans] 0.0
blast AAO91710 Transbilayer amphipath transporters (subfamily iv p-type atpase) protein 2, isoform b [Caenorhabditis elegans] ref|NP_001023253.1| H06H21.10b [Caenorhabditis elegans] 0.0
blast CAE66400 Hypothetical protein CBG11664 [Caenorhabditis briggsae] 0.0
Protein Families
Method ID Description E-Value
ec A8B2_HUMAN (P98198) Potential phospholipid-transporting ATPase ID (EC 3.6.3.1) (ATPase class I type 8B member 2) 0.0
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001023253.1 8 20-42,74-96,649-671,678-700,733-755,768-790,800-822,846-868
NP_001023252.1 8 191-213,245-267,820-842,849-871,904-926,939-961,971-993,1017-1039
H06H21.10b 8 20-42,74-96,649-671,678-700,733-755,768-790,800-822,846-868
H06H21.10a 8 191-213,245-267,820-842,849-871,904-926,939-961,971-993,1017-1039
Sequence
Target Sequence 
>C. ELEGANS:187523_S_AT
tcctcacttctggttttcaattctaatggtttcagttgtactgttactaccagttatgct
taatcgattcttctggcttgatacacatccatcatttgctgataggctgagaattcgaaa
gaaaatgggcaagaaaccatcggcgaaagatgataaaaaaaccgcattcaaacgcacggc
agcaactcgacgaagtgtccgtggatcacttagaagtggttacgcattctctcattcaca
aggattcggagaactcattctcaaaggaaaattgttcaaaaatgtggaaaatctacgggg
aaagaataattcgaatgcgaaaattcacccgacttctgatgacttgcagccgatgcttat
ttctagtgtgcctgatgacagccaaggagcttcaagtattaatgcaatgcaccttccaat
gggtacacgtccacagaatgtaccccatacattgaatgtaaatactgatgactggtctca
atcatcggatttccgtccagcctatgcaaaggaaccatcaccactgcagggtacagtaat
ccgtggcgat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCCTCACTTCTGGTTTTCAATTCTA 83 593 1413 Antisense
GATTCTTCTGGCTTGATACACATCC 557 431 1478 Antisense
GCATTCAAACGCACGGCAGCAACTC 517 307 1576 Antisense
GCAGCAACTCGACGAAGTGTCCGTG 381 313 1591 Antisense
GAAGTGGTTACGCATTCTCTCATTC 649 335 1625 Antisense
AATTCACCCGACTTCTGATGACTTG 62 177 1734 Antisense
GCCGATGCTTATTTCTAGTGTGCCT 582 283 1761 Antisense
TGCACCTTCCAATGGGTACACGTCC 65 567 1820 Antisense
GGTCTCAATCATCGGATTTCCGTCC 423 501 1886 Antisense
ATTTCCGTCCAGCCTATGCAAAGGA 579 11 1901 Antisense
GCAGGGTACAGTAATCCGTGGCGAT 543 309 1938 Antisense
Sequence Source Affymetrix Proprietary Database