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[187519_at]

Description B0303.2 /REP_DB=WormBase Gene ID /WP=CE24764 /TR=SW:P34254 /GB=AAA27896.2 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 34      6grp_high1_10_10 > 32      4grp_highest_8_8 > 01      4grp_high_8_8 > 45      6grp_highest_nonorm > 71      6grp_total > 21      4grp_total > 50      6grp_highest_sub > 02_03      6grp_high1_sub > 02_03      6grp_total_sub > Hat      6grp_rule_based > S2      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 378
1 2 3 4 range
A 202.4
(-1.8)
P 		474.8
(3)
P 		318.4
(0.3)
P 		131.4
(-3)
P 		343
B 197.5
(-2.1)
P 		509.8
(3)
P 		293.7
(-0.5)
P 		143
(-3)
P 		367
C 164.2
(-3)
P 		486
(3)
P 		480.1
(2.9)
P 		161.8
(-3)
P 		324
avr 188
(-2.3) 		490.2
(3) 		364.1
(0.8) 		145.4
(-3) 		345

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 556
1 2 3 4 5 6 range
A 341.3
(-0.5)
P 		436.8
(0.5)
P 		667.8
(3)
P 		291.3
(-1.1)
P 		203.4
(-2)
P 		112.3
(-3)
P 		556
B 443.5
(0.9)
P 		557
(2.6)
P 		585.8
(3)
P 		360.8
(-0.3)
P 		176.4
(-2.9)
P 		171.2
(-3)
P 		415
C 317.6
(-0.8)
P 		607.9
(3)
P 		338.4
(-0.6)
P 		318.6
(-0.8)
P 		154.9
(-3)
P 		180.2
(-2.7)
P 		453
avr 367.5
(0.4) 		533.9
(3) 		530.7
(2.9) 		323.6
(-0.3) 		178.2
(-2.6) 		154.6
(-3) 		379
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 148
1 2 3 4 range
A 73.7
(0.9)
P 		103.1
(3)
P 		83.2
(1.6)
P 		19.2
(-3)
P 		84
B 63
(-1)
P 		125.9
(3)
P 		68.1
(-0.6)
P 		30.5
(-3)
P 		95
C 54.4
(-1.8)
P 		139.7
(1.8)
P 		167.3
(3)
P 		27.3
(-3)
P 		140
avr 63.7
(-0.7) 		122.9
(3) 		106.2
(2) 		25.7
(-3) 		97

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 158
1 2 3 4 5 6 range
A 62.9
(-1.3)
P 		155.4
(3)
P 		145.4
(2.5)
P 		73.9
(-0.8)
P 		61.4
(-1.4)
P 		27.3
(-3)
P 		128
B 154.3
(1.5)
P 		185.4
(3)
P 		142.3
(0.9)
P 		95.6
(-1.4)
P 		71.7
(-2.6)
P 		63.8
(-3)
P 		122
C 117.7
(-0)
P 		185.5
(3)
P 		86.1
(-1.4)
P 		64.1
(-2.4)
P 		50.1
(-3)
P 		60.3
(-2.5)
P 		135
avr 111.6
(-0.1) 		175.4
(3) 		124.6
(0.6) 		77.9
(-1.7) 		61.1
(-2.5) 		50.5
(-3) 		125
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187519_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7558
Sequence Type Exemplar sequence
Representative Public ID B0303.2 NCBI
Target Description B0303.2 /REP_DB=WormBase Gene ID /WP=CE24764 /TR=SW:P34254 /GB=AAA27896.2 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066513(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066513 NCBI Caenorhabditis elegans B0303.2 (B0303.2) mRNA, complete cds. 9/11 None
SNAP00000042402 ENSEMBL cdna:SNAP chromosome:CEL140:III:8684711:8686228:1 9/11 None
GENEFINDER00000042414 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:8684711:8686228:1 9/11 None
B0303.2 ENSEMBL cdna:known chromosome:CEL140:III:8684710:8686280:1 gene:B0303.2 9/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8684705-8686227(+) 99.58 100.0
Public Domain and Genome References
Gene Title
Ensembl B0303.2
Entrez Gene 176217 Entrez gene
SwissProt P34254 EMBL-EBI
WormBase CE00536 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
8168 methyltransferase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA27896 Hypothetical protein B0303.2 [Caenorhabditis elegans] ref|NP_498914.1| B0303.2 [Caenorhabditis elegans] sp|P34254|YKA2_CAEEL Hypothetical protein B0303.2 in chromosome III 1.0E-157
blast CAE57607 Hypothetical protein CBG00588 [Caenorhabditis briggsae] 1.0E-132
blast AAT11866 putative phenylethanolamine N-methyltransferase isoform a [Dictyocaulus viviparus] gb|AAT11865.1| putative phenylethanolamine N-methyltransferase [Dictyocaulus viviparus] 8.0E-37
Protein Domains
Database ID Description E-Value
Pfam IPR000940 EMBL-EBI Methyltransferase, NNMT/PNMT/TEMT 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:187519_AT
gatgtgaacgtccatgaatctccggttgttcaatcaattgtctggaaagaaaatgagcaa
gttcaagttccagacaaatttcaagttgtatcaactgtcttctgtctagagtattcatgt
gaaactcttgaagcatatttccgggcggtacgaagtgcgtgttcccttattgatgaaggt
ggtattctgattcaaggtggtgttctcgatgcaacgacctacaattttggaggaaaaaca
tttcgatgtcatcggctgaaacaagcacatataattgaaagtctcaaggtaaattgtatt
tttgaaaaaagtgttttaaaatattattctttgaaaaaaaagatccaccaacaagagcaa
aaaatttcaaaatgttccaaattattctcccaaccatatgatttcaggcaaatggtatgg
cgacaactgccgaacaag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATGTGAACGTCCATGAATCTCCGG 413 409 460 Antisense
GAATCTCCGGTTGTTCAATCAATTG 171 327 475 Antisense
GTTGTATCAACTGTCTTCTGTCTAG 699 437 544 Antisense
TTGAAGCATATTTCCGGGCGGTACG 307 703 587 Antisense
GGGCGGTACGAAGTGCGTGTTCCCT 700 489 602 Antisense
GAAGTGCGTGTTCCCTTATTGATGA 662 335 611 Antisense
GATTCAAGGTGGTGTTCTCGATGCA 135 431 648 Antisense
GATGCAACGACCTACAATTTTGGAG 623 411 667 Antisense
ATGTTCCAAATTATTCTCCCAACCA 178 47 831 Antisense
TTCTCCCAACCATATGATTTCAGGC 201 693 844 Antisense
GGTATGGCGACAACTGCCGAACAAG 506 503 873 Antisense
Sequence Source Affymetrix Proprietary Database