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[187507_at]

Description C32D5.4 /REP_DB=WormBase Gene ID /WP=CE01844 /TR=SW:Q09264 /GB=AAC46792.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 97      4grp_total > 26      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 43
1 2 3 4 range
A 6.4
(-3)
A 		36.4
(1.7)
A 		8.4
(-2.7)
A 		44.8
(3)
A 		38
B 2.1
(-3)
A 		26.5
(1)
A 		5.5
(-2.4)
A 		38.9
(3)
A 		37
C 16.7
(0.2)
A 		10.8
(-1.5)
A 		26.7
(3)
A 		5.6
(-3)
A 		21
avr 8.4
(-3) 		24.6
(1.5) 		13.5
(-1.6) 		29.8
(3) 		21

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 115
1 2 3 4 5 6 range
A 13.8
(-2.7)
A 		8.1
(-3)
A 		20.5
(-2.3)
A 		30.5
(-1.8)
A 		78.4
(0.8)
P 		119.9
(3)
P 		112
B 28.4
(-2.7)
A 		24.1
(-3)
A 		46.1
(-1.4)
A 		38.9
(-1.9)
A 		105.3
(3)
P 		97.4
(2.4)
P 		81
C 5.9
(-3)
A 		24.1
(-2)
A 		5.2
(-3)
A 		52.5
(-0.4)
A 		86.9
(1.5)
P 		114.2
(3)
P 		109
avr 16
(-3) 		18.8
(-2.8) 		23.9
(-2.5) 		40.6
(-1.4) 		90.2
(1.7) 		110.5
(3) 		95
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9
1 2 3 4 range
A 2.3
(-2.9)
A 		7.9
(3)
A 		2.2
(-3)
A 		6.6
(1.6)
A 		6
B 0.7
(-3)
A 		6.6
(1.7)
A 		1.3
(-2.5)
A 		8.3
(3)
A 		8
C 5.5
(0.3)
A 		3.1
(-1.4)
A 		9.3
(3)
A 		0.9
(-3)
A 		8
avr 2.8
(-3) 		5.9
(3) 		4.3
(-0.1) 		5.3
(1.8) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 42
1 2 3 4 5 6 range
A 2.6
(-3)
A 		2.9
(-2.9)
A 		4.5
(-2.6)
A 		7.7
(-1.8)
A 		23.6
(1.7)
P 		29.2
(3)
P 		27
B 9.9
(-2.7)
A 		8
(-3)
A 		11.2
(-2.4)
A 		10.3
(-2.6)
A 		42.8
(3)
P 		36.3
(1.9)
P 		35
C 2.2
(-2.9)
A 		7.4
(-2)
A 		1.3
(-3)
A 		10.6
(-1.5)
A 		28.1
(1.4)
P 		38.2
(3)
P 		37
avr 4.9
(-3) 		6.1
(-2.8) 		5.7
(-2.8) 		9.5
(-2.1) 		31.5
(2.4) 		34.6
(3) 		30
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187507_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5703
Sequence Type Exemplar sequence
Representative Public ID C32D5.4 NCBI
Target Description C32D5.4 /REP_DB=WormBase Gene ID /WP=CE01844 /TR=SW:Q09264 /GB=AAC46792.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062869(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062869 NCBI Caenorhabditis elegans C32D5.4 (C32D5.4) mRNA, complete cds. 11/11 None
SNAP00000026825 ENSEMBL cdna:SNAP chromosome:CEL140:II:6318130:6322741:-1 11/11 None
GENEFINDER00000026841 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:6321345:6323026:-1 11/11 None
C32D5.4 ENSEMBL cdna:known chromosome:CEL140:II:6321309:6323026:-1 gene:C32D5.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:6321341-6323023(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C32D5.4
Entrez Gene 174043 Entrez gene
SwissProt Q09264 EMBL-EBI
WormBase CE01844 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC46792 Hypothetical protein C32D5.4 [Caenorhabditis elegans] ref|NP_495270.1| C32D5.4 [Caenorhabditis elegans] sp|Q09264|YQD4_CAEEL Hypothetical protein C32D5.4 in chromosome II 0.0
blast AAC46791 Hypothetical protein C32D5.3 [Caenorhabditis elegans] ref|NP_495269.1| C32D5.3 [Caenorhabditis elegans] sp|Q09263|YQD3_CAEEL Hypothetical protein C32D5.3 in chromosome II 0.0
blast CAE59290 Hypothetical protein CBG02625 [Caenorhabditis briggsae] 0.0
blast AAC46792 Hypothetical protein C32D5.4 [Caenorhabditis elegans] ref|NP_495270.1| C32D5.4 [Caenorhabditis elegans] sp|Q09264|YQD4_CAEEL Hypothetical protein C32D5.4 in chromosome II 1.0E-156
blast CAE59291 Hypothetical protein CBG02626 [Caenorhabditis briggsae] 4.0E-91
Sequence
Target Sequence 
>C. ELEGANS:187507_AT
acaacgcatctggtcatcaagtatcattttgaatagtgcagaacttgaatatcccgaagt
caagtacaagctacgtgtcagcgttaaaaagaagtttgatccaaaattgagctgcaacag
aaataagggaacaatcaagcgaaatgatgtattcgtgttgacgtaccaacttcctgaatc
atcaatcgaagctgtccgtctgacacttattgttgatggaaatccacatgaaattcctct
ttgtaaatataaaaaggcagattattattggcttggaaaagaaacaaatgttcccgggcc
aactgaattgcttcgagaacggaacgttagcccgaatacagcctccacaattaagaatgt
gtctccaggacgcatgattgatttaccagaaggacttaaaaacttcgtaattggtgaaaa
tggtcttgaatccatctgcaaactacttcatcctcacgctgtctcactttttcgagac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACAACGCATCTGGTCATCAAGTATC 422 111 498 Antisense
TAGTGCAGAACTTGAATATCCCGAA 100 655 531 Antisense
GTACAAGCTACGTGTCAGCGTTAAA 286 461 561 Antisense
TGATGTATTCGTGTTGACGTACCAA 367 563 642 Antisense
GACGTACCAACTTCCTGAATCATCA 124 379 657 Antisense
GAAGCTGTCCGTCTGACACTTATTG 384 343 685 Antisense
CCGGGCCAACTGAATTGCTTCGAGA 309 265 791 Antisense
CGGAACGTTAGCCCGAATACAGCCT 88 241 817 Antisense
GAATGTGTCTCCAGGACGCATGATT 224 333 852 Antisense
AATGGTCTTGAATCCATCTGCAAAC 208 163 916 Antisense
TCACGCTGTCTCACTTTTTCGAGAC 436 625 951 Antisense
Sequence Source Affymetrix Proprietary Database