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[187502_s_at]

Description K02A2.1 /REP_DB=WormBase Gene ID /WP=CE21012 /TR=SW:Q09571 /GB=AAC46705.2 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 12      6grp_total > 23      4grp_total > 14      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 186
1 2 3 4 range
A 186.1
(2.3)
P 		97.2
(-3)
A 		129.3
(-1.1)
A 		197.7
(3)
P 		101
B 181.1
(3)
P 		135.1
(-2.3)
A 		128.8
(-3)
A 		154.7
(-0)
P 		52
C 210.6
(0.4)
P 		128.6
(-2.6)
P 		118.3
(-3)
M 		283.5
(3)
P 		165
avr 192.6
(1.7) 		120.3
(-3) 		125.5
(-2.7) 		212
(3) 		92

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 398
1 2 3 4 5 6 range
A 459
(2.5)
P 		436
(2.2)
P 		490.1
(3)
P 		233.1
(-0.9)
P 		155.4
(-2)
P 		92.4
(-3)
M 		398
B 481.4
(3)
P 		434.3
(2.3)
P 		381.7
(1.4)
P 		264
(-0.5)
P 		187.6
(-1.7)
P 		103.4
(-3)
P 		378
C 445.1
(3)
P 		443.7
(3)
P 		424.3
(2.6)
P 		252.9
(-0.5)
A 		185
(-1.8)
P 		119.7
(-3)
P 		325
avr 461.8
(3) 		438
(2.6) 		432
(2.5) 		250
(-0.6) 		176
(-1.8) 		105.2
(-3) 		357
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 49
1 2 3 4 range
A 67.7
(3)
P 		21.1
(-3)
A 		33.8
(-1.4)
A 		28.9
(-2)
P 		47
B 57.8
(3)
P 		33.4
(-2.2)
A 		29.9
(-3)
A 		33
(-2.3)
P 		28
C 69.7
(3)
P 		37
(-3)
P 		41.2
(-2.2)
M 		47.8
(-1)
P 		33
avr 65.1
(3) 		30.5
(-3) 		35
(-2.2) 		36.6
(-1.9) 		35

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 145
1 2 3 4 5 6 range
A 84.6
(-0.2)
P 		155
(3)
P 		106.7
(0.8)
P 		59.1
(-1.3)
P 		46.9
(-1.9)
P 		22.5
(-3)
M 		133
B 167.5
(3)
P 		144.5
(1.9)
P 		92.7
(-0.5)
P 		69.9
(-1.5)
P 		76.2
(-1.2)
P 		38.5
(-3)
P 		129
C 165
(3)
P 		135.4
(1.6)
P 		108
(0.3)
P 		50.9
(-2.5)
A 		59.9
(-2)
P 		40.1
(-3)
P 		125
avr 139
(2.7) 		145
(3) 		102.5
(0.7) 		60
(-1.6) 		61
(-1.5) 		33.7
(-3) 		111
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 187502_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5670
Sequence Type Exemplar sequence
Representative Public ID K02A2.1 NCBI
Target Description K02A2.1 /REP_DB=WormBase Gene ID /WP=CE21012 /TR=SW:Q09571 /GB=AAC46705.2 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063159(11), NM_063155(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063159 NCBI Caenorhabditis elegans D1022.3 (D1022.3) mRNA, complete cds. 11/11 A 
NM_063155 NCBI Caenorhabditis elegans K02A2.1 (K02A2.1) mRNA, complete cds. 11/11 None
SNAP00000004643 ENSEMBL cdna:SNAP chromosome:CEL140:II:7455172:7456138:-1 11/11 None
GENEFINDER00000004660 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:7455172:7456138:-1 11/11 None
SNAP00000023850 ENSEMBL cdna:SNAP chromosome:CEL140:II:7435187:7435714:1 11/11 None
GENEFINDER00000023856 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:7432626:7435714:1 11/11 None
K02A2.1 ENSEMBL cdna:known chromosome:CEL140:II:7435187:7435714:1 gene:K02A2.1 11/11 None
D1022.3 ENSEMBL cdna:known chromosome:CEL140:II:7455172:7456138:-1 gene:D1022.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7435183-7435711(+) 100.0 100.0
chrII:7455168-7455696(-) 97.69 97.69
Public Domain and Genome References
Gene Title

Ensembl K02A2.1
D1022.3
Entrez Gene 183900 Entrez gene
186856 Entrez gene
SwissProt Q18925 EMBL-EBI
Q09571 EMBL-EBI
WormBase CE02577 Wormbase
CE21012 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
16787 hydrolase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_495560 D1022.3 [Caenorhabditis elegans] gb|AAB93336.1| Hypothetical protein D1022.3 [Caenorhabditis elegans] 1.0E-139
blast NP_495560 D1022.3 [Caenorhabditis elegans] gb|AAB93336.1| Hypothetical protein D1022.3 [Caenorhabditis elegans] 1.0E-102
blast AAC46705 Hypothetical protein K02A2.1 [Caenorhabditis elegans] ref|NP_495556.1| K02A2.1 [Caenorhabditis elegans] sp|Q09571|YRD1_CAEEL Hypothetical protein K02A2.1 in chromosome II 6.0E-88
blast AAC46705 Hypothetical protein K02A2.1 [Caenorhabditis elegans] ref|NP_495556.1| K02A2.1 [Caenorhabditis elegans] sp|Q09571|YRD1_CAEEL Hypothetical protein K02A2.1 in chromosome II 4.0E-87
blast NP_495560 D1022.3 [Caenorhabditis elegans] gb|AAB93336.1| Hypothetical protein D1022.3 [Caenorhabditis elegans] 2.0E-83
Protein Domains
Database ID Description E-Value
Pfam IPR002925 EMBL-EBI Dienelactone hydrolase 1.1E-53
Pfam IPR002925 EMBL-EBI Dienelactone hydrolase 3.2E-38
Pfam IPR002925 EMBL-EBI Dienelactone hydrolase 2.0E-45
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000023856 4 15-37,50-69,101-123,135-154
Sequence
Target Sequence 
>C. ELEGANS:187502_S_AT
tagaagctgctttaaatgccttgaaatctgttccatgtgtagataaacagaagctcggtg
catttggattctgtattggaggactgtgttccctggattgtgctcgttatagatttgatg
gaattcgagctgttataagttttcatgggactctgacccctatcgaaggaattcctttag
aactgttagatgataattctattcaagttcaccatggagatgctgacaaacacgtttcaa
aagtcaccgttgacgcattccacgaagaaatgagagcaagaaattccgactttgttttta
tttctcatgggaaagcaatgcattcgttcactgatcctgagtctgctggaattgccccat
ctggagtcggttacgatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TAGAAGCTGCTTTAAATGCCTTGAA 57 651 50 Antisense
ATGCCTTGAAATCTGTTCCATGTGT 610 39 65 Antisense
AAGCTCGGTGCATTTGGATTCTGTA 616 149 100 Antisense
GTATTGGAGGACTGTGTTCCCTGGA 440 451 122 Antisense
TTCCCTGGATTGTGCTCGTTATAGA 391 697 138 Antisense
ATTCGAGCTGTTATAAGTTTTCATG 592 9 172 Antisense
CTCTGACCCCTATCGAAGGAATTCC 239 227 200 Antisense
TCACCGTTGACGCATTCCACGAAGA 119 625 293 Antisense
GAGCAAGAAATTCCGACTTTGTTTT 146 391 323 Antisense
AGCAATGCATTCGTTCACTGATCCT 105 77 363 Antisense
GCCCCATCTGGAGTCGGTTACGATG 628 283 403 Antisense
Sequence Source Affymetrix Proprietary Database