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[187495_s_at] acyl-coA dehydrogenase

Description F28A10.6 /REP_DB=WormBase Gene ID /WP=CE19411 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=acyl-coA dehydrogenase
Cluster 6grp_highest_10_10 > 24      6grp_high1_10_10 > 30      4grp_highest_8_8 > 11      4grp_high_8_8 > 47      6grp_highest_nonorm > 42      6grp_total > 42      4grp_total > 49      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 403
1 2 3 4 range
A 931.6
(-3)
P 		1297.7
(3)
P 		1270.2
(2.5)
P 		1111.4
(-0.1)
P 		366
B 1179.8
(-1)
P 		1240.5
(2.4)
P 		1251.8
(3)
P 		1143.7
(-3)
P 		108
C 975.5
(-3)
P 		1209.7
(0.9)
P 		1334.7
(3)
P 		1047.5
(-1.8)
P 		359
avr 1029
(-3) 		1249.3
(2.2) 		1285.6
(3) 		1100.9
(-1.3) 		257

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 977
1 2 3 4 5 6 range
A 1129.1
(1.3)
P 		1099.7
(1.1)
P 		1353.5
(3)
P 		886.6
(-0.6)
P 		565.6
(-3)
P 		703.9
(-1.9)
P 		788
B 983.7
(-0.7)
P 		891.9
(-1.4)
P 		1542.5
(3)
P 		1017.2
(-0.5)
P 		647.6
(-3)
P 		781.5
(-2.1)
P 		895
C 770.9
(-1.5)
P 		1152.1
(2.5)
P 		1204.5
(3)
P 		981.3
(0.7)
P 		631.2
(-3)
P 		656.6
(-2.7)
P 		573
avr 961.2
(-0.2) 		1047.9
(0.5) 		1366.8
(3) 		961.7
(-0.2) 		614.8
(-3) 		714
(-2.2) 		752
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 303
1 2 3 4 range
A 339.2
(3)
P 		281.7
(1)
P 		332.1
(2.8)
P 		162.7
(-3)
P 		177
B 376.2
(3)
P 		306.4
(-0.2)
P 		290.1
(-0.9)
P 		243.7
(-3)
P 		133
C 322.9
(0)
P 		347.8
(0.6)
P 		465.2
(3)
P 		176.6
(-3)
P 		289
avr 346.1
(2.4) 		312
(1.2) 		362.5
(3) 		194.3
(-3) 		168

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 220
1 2 3 4 5 6 range
A 208.1
(-2)
P 		391.1
(3)
P 		294.8
(0.4)
P 		224.9
(-1.5)
P 		170.7
(-3)
P 		171.4
(-3)
P 		220
B 342.3
(1.3)
P 		296.9
(-1.2)
P 		374.6
(3)
P 		269.4
(-2.7)
P 		263.1
(-3)
P 		291.4
(-1.5)
P 		112
C 285.7
(0.4)
P 		351.5
(3)
P 		306.6
(1.3)
P 		197.3
(-3)
P 		204.3
(-2.7)
P 		219.8
(-2.1)
P 		154
avr 278.7
(-0) 		346.5
(3) 		325.3
(2) 		230.5
(-2.2) 		212.7
(-3) 		227.5
(-2.3) 		134
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 187495_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5887
Sequence Type Exemplar sequence
Representative Public ID F28A10.6 NCBI
Target Description F28A10.6 /REP_DB=WormBase Gene ID /WP=CE19411 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=acyl-coA dehydrogenase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061431(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061431 NCBI Caenorhabditis elegans F28A10.6 (F28A10.6) mRNA, complete cds. 11/11 None
SNAP00000009515 ENSEMBL cdna:SNAP chromosome:CEL140:II:846977:848606:-1 11/11 None
F28A10.6 ENSEMBL cdna:known chromosome:CEL140:II:846897:848608:-1 gene:F28A10.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:846976-848606(-) 100.0 100.0
Public Domain and Genome References
Gene Title acyl-coA dehydrogenase
Ensembl F28A10.6
Entrez Gene 173466 Entrez gene
SwissProt Q95YB2 EMBL-EBI
WormBase CE19411 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.15329.1.S1_AT similar to Acyl-CoA dehydrogenase family member 8, mitochondrial precursor (ACAD-8) (Isobutyryl-CoA dehydrogenase) (Activator-recruited cofactor 42 kDa component) (ARC42) cfa
CHICKEN:GGA.16393.1.S1_AT acyl-Coenzyme A dehydrogenase family, member 8 gga
HG-U133_PLUS_2:221669_S_AT acyl-Coenzyme A dehydrogenase family, member 8 hs
HG-U133A_2:221669_S_AT acyl-Coenzyme A dehydrogenase family, member 8 hs
HG-U133A:221669_S_AT acyl-Coenzyme A dehydrogenase family, member 8 hs
HG-FOCUS:221669_S_AT acyl-Coenzyme A dehydrogenase family, member 8 hs
HU35KSUBB:RC_H09750_AT acyl-Coenzyme A dehydrogenase family, member 8 hs
U133_X3P:G12805020_3P_A_AT acyl-Coenzyme A dehydrogenase family, member 8 hs
HG-U95C:63383_AT acyl-Coenzyme A dehydrogenase family, member 8 hs
HG-U95E:77945_AT acyl-Coenzyme A dehydrogenase family, member 8 hs
HU35KSUBC:RC_N26556_AT Acyl-Coenzyme A dehydrogenase family, member 8 hs
HG-U95B:54278_AT Acyl-Coenzyme A dehydrogenase family, member 8 hs
HG-U133_PLUS_2:238175_AT Acyl-Coenzyme A dehydrogenase family, member 8 hs
HG-U133B:238175_AT Acyl-Coenzyme A dehydrogenase family, member 8 hs
HG-U95B:55898_AT Acyl-Coenzyme A dehydrogenase family, member 8 hs
HU35KSUBC:RC_D20772_AT Acyl-Coenzyme A dehydrogenase family, member 8 hs
HU35KSUBC:RC_F09156_AT Acyl-Coenzyme A dehydrogenase family, member 8 hs
U133_X3P:HS.66095.0.A1_3P_AT Acyl-Coenzyme A dehydrogenase family, member 8 hs
MG-U74BV2:163813_AT acyl-Coenzyme A dehydrogenase family, member 8 mm
MG-U74BV2:163010_AT acyl-Coenzyme A dehydrogenase family, member 8 mm
MG-U74CV2:168164_R_AT acyl-Coenzyme A dehydrogenase family, member 8 mm
MOE430A:1419261_AT acyl-Coenzyme A dehydrogenase family, member 8 mm
MOUSE430A_2:1419261_AT acyl-Coenzyme A dehydrogenase family, member 8 mm
MOUSE430_2:1419261_AT acyl-Coenzyme A dehydrogenase family, member 8 mm
MOE430A:1419262_AT acyl-Coenzyme A dehydrogenase family, member 8 mm
MOUSE430A_2:1419262_AT acyl-Coenzyme A dehydrogenase family, member 8 mm
MOUSE430_2:1419262_AT acyl-Coenzyme A dehydrogenase family, member 8 mm
MU19KSUBB:TC30840_AT Acyl-Coenzyme A dehydrogenase family, member 8, mRNA (cDNA clone MGC:46786 IMAGE:5053565) mm
MU19KSUBB:TC30840_G_AT Acyl-Coenzyme A dehydrogenase family, member 8, mRNA (cDNA clone MGC:46786 IMAGE:5053565) mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3995 acyl-CoA dehydrogenase activity inferred from electronic annotation QuickGO AmiGO
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK85472 Hypothetical protein F28A10.6 [Caenorhabditis elegans] ref|NP_493832.1| F28A10.6 [Caenorhabditis elegans] 0.0
blast CAE62925 Hypothetical protein CBG07125 [Caenorhabditis briggsae] 0.0
blast AAH66503 Acad8 protein [Danio rerio] 1.0E-134
Protein Families
Method ID Description E-Value
ec ACDM_HUMAN (P11310) Acyl-CoA dehydrogenase, medium-chain specific, mitochondrial precursor (EC 1.3.99.3) (MCAD) 1.0E-137
Protein Domains
Database ID Description E-Value
Pfam IPR006090 EMBL-EBI Acyl-CoA dehydrogenase, C-terminal 1.6E-69
Pfam IPR006091 EMBL-EBI Acyl-CoA dehydrogenase, central domain 8.5E-41
Pfam IPR006092 EMBL-EBI Acyl-CoA dehydrogenase, N-terminal 1.9E-15
Sequence
Target Sequence 
>C. ELEGANS:187495_S_AT
gaaaagacgggttcggcttcaacatcgcaatggctgggctcaacggaggacgcattaaca
ttgcgagttgctcactaggagctgctcaaaggagcatggacttagctattgaacatttga
aatatagaaaacaatttggtaagagcttggcagacttccagtataaccagttcaagcttg
cggagctcgccacaaaattgtatactagcagactaatcgttagaaacgccgcggagcaac
tagacaatgatgatccggagaaggtggctctttgtgcaatggcgaagcttcacgccactg
ataattgtttcgatgtcgtcaacggagctctgcagatgttcggtggctacggattcctga
aagattatccggttcaacagtacctcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAAAGACGGGTTCGGCTTCAACAT 635 149 755 Antisense
TCAACATCGCAATGGCTGGGCTCAA 229 631 773 Antisense
GCGAGTTGCTCACTAGGAGCTGCTC 181 291 817 Antisense
GGCAGACTTCCAGTATAACCAGTTC 557 535 903 Antisense
ACCAGTTCAAGCTTGCGGAGCTCGC 631 83 920 Antisense
AAACGCCGCGGAGCAACTAGACAAT 581 125 978 Antisense
TCCGGAGAAGGTGGCTCTTTGTGCA 426 593 1008 Antisense
GAAGCTTCACGCCACTGATAATTGT 198 343 1038 Antisense
GTCAACGGAGCTCTGCAGATGTTCG 7 465 1072 Antisense
AGATGTTCGGTGGCTACGGATTCCT 630 67 1088 Antisense
GATTATCCGGTTCAACAGTACCTCC 221 425 1117 Antisense
Sequence Source Affymetrix Proprietary Database