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[187482_at]

Description K10D2.4 /REP_DB=WormBase Gene ID /WP=CE02016 /TR=SW:Q09410 /GB=AAA62543.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 88      6grp_high1_10_10 > 88      4grp_highest_8_8 > 14      4grp_high_8_8 > 22      6grp_highest_nonorm > 93      6grp_total > 97      4grp_total > 16      6grp_highest_sub > Da      6grp_high1_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 255
1 2 3 4 range
A 196.3
(-3)
P 		256
(-1.5)
P 		315.1
(0)
P 		433.4
(3)
P 		237
B 189.2
(-3)
P 		241.7
(-1.6)
P 		273.8
(-0.8)
P 		420.5
(3)
P 		231
C 229.3
(-3)
P 		290.9
(-1.3)
P 		273.8
(-1.8)
P 		443.7
(3)
P 		214
avr 204.9
(-3) 		262.9
(-1.5) 		287.6
(-0.8) 		432.5
(3) 		228

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 482
1 2 3 4 5 6 range
A 245.8
(-3)
P 		267.1
(-2.7)
P 		348.5
(-1.4)
P 		353.6
(-1.3)
P 		390.1
(-0.7)
P 		628.9
(3)
P 		383
B 252.6
(-2.5)
P 		214
(-3)
P 		297.6
(-2)
P 		264.1
(-2.4)
P 		500.3
(0.6)
P 		695.9
(3)
P 		482
C 279.6
(-2.1)
P 		222
(-3)
P 		240.8
(-2.7)
P 		259.6
(-2.4)
P 		570.8
(2.2)
P 		620.8
(3)
P 		399
avr 259.3
(-2.6) 		234.4
(-3) 		295.6
(-2.1) 		292.4
(-2.2) 		487.1
(0.7) 		648.5
(3) 		414
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 40
1 2 3 4 range
A 71.5
(0.6)
P 		55.6
(-3)
P 		82.4
(3)
P 		63.4
(-1.3)
P 		27
B 60.3
(-2.9)
P 		59.7
(-3)
P 		63.4
(-2.3)
P 		89.6
(3)
P 		30
C 75.9
(-2.7)
P 		83.6
(-0.4)
P 		95.4
(3)
P 		74.8
(-3)
P 		21
avr 69.2
(-1.8) 		66.3
(-3) 		80.4
(3) 		75.9
(1.1) 		14

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 214
1 2 3 4 5 6 range
A 45.3
(-3)
P 		95
(-0.2)
P 		75.9
(-1.3)
P 		89.7
(-0.5)
P 		117.7
(1)
P 		153.1
(3)
P 		108
B 87.9
(-2.4)
P 		71.2
(-3)
P 		72.3
(-2.9)
P 		69.9
(-3)
P 		203.3
(1.2)
P 		259.5
(3)
P 		190
C 103.6
(-1)
P 		67.7
(-2.4)
P 		61.3
(-2.6)
P 		52.2
(-3)
P 		184.7
(2.1)
P 		207.8
(3)
P 		156
avr 78.9
(-2.6) 		78
(-2.6) 		69.8
(-3) 		70.6
(-3) 		168.6
(1.3) 		206.8
(3) 		137
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187482_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6258
Sequence Type Exemplar sequence
Representative Public ID K10D2.4 NCBI
Target Description K10D2.4 /REP_DB=WormBase Gene ID /WP=CE02016 /TR=SW:Q09410 /GB=AAA62543.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065697(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065697 NCBI Caenorhabditis elegans K10D2.4 (K10D2.4) mRNA, complete cds. 11/11 None
SNAP00000029356 ENSEMBL cdna:SNAP chromosome:CEL140:III:5173403:5173717:1 11/11 None
GENEFINDER00000029364 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:5173403:5173717:1 11/11 None
K10D2.4 ENSEMBL cdna:known chromosome:CEL140:III:5173396:5173768:1 gene:K10D2.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:5173401-5173716(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl K10D2.4
Entrez Gene 187262 Entrez gene
SwissProt Q09410 EMBL-EBI
WormBase CE02016 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA62543 Hypothetical protein K10D2.4 [Caenorhabditis elegans] ref|NP_498098.1| K10D2.4 [Caenorhabditis elegans] sp|Q09410|YRJ4_CAEEL Hypothetical protein K10D2.4 in chromosome III 1.0E-42
blast CAE73781 Hypothetical protein CBG21326 [Caenorhabditis briggsae] 4.0E-31
Sequence
Target Sequence 
>C. ELEGANS:187482_AT
aactggtccgagcacttgtgggtttccgaagtctcaccagcaaaagagtcattcatcacc
acgatttgtgagcaccgtcaagctcaatgggataatcaagatcttctgcgtcacttgcaa
gacagtgtggctattctgactcgtgaagatcaacgtcatgtgaacgctgtccat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACTGGTCCGAGCACTTGTGGGTTT 649 137 55 Antisense
TTGTGGGTTTCCGAAGTCTCACCAG 202 707 70 Antisense
TTTCCGAAGTCTCACCAGCAAAAGA 105 669 77 Antisense
AAAAGAGTCATTCATCACCACGATT 670 129 96 Antisense
TCACCACGATTTGTGAGCACCGTCA 2 627 110 Antisense
CCGTCAAGCTCAATGGGATAATCAA 397 265 129 Antisense
GGATAATCAAGATCTTCTGCGTCAC 398 511 144 Antisense
GATCTTCTGCGTCACTTGCAAGACA 636 419 154 Antisense
GACAGTGTGGCTATTCTGACTCGTG 187 369 175 Antisense
GACTCGTGAAGATCAACGTCATGTG 483 373 192 Antisense
TCAACGTCATGTGAACGCTGTCCAT 198 631 204 Antisense
Sequence Source Affymetrix Proprietary Database