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[187466_at]

Description B0303.4 /REP_DB=WormBase Gene ID /WP=CE00537 /TR=SW:P34256 /GB=AAA27898.2 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 96      6grp_high1_10_10 > 69      4grp_highest_8_8 > 38      4grp_high_8_8 > 03      6grp_highest_nonorm > 91      6grp_total > 79      4grp_total > 05      6grp_highest_sub > Cu      6grp_high1_sub > Cu      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 231
1 2 3 4 range
A 245.1
(2.6)
P 		140.5
(-3)
P 		193.6
(-0.1)
P 		251.7
(3)
P 		111
B 193.8
(-1.2)
P 		130.5
(-2.9)
P 		125.9
(-3)
P 		356.8
(3)
P 		231
C 189.5
(-0.7)
P 		143.4
(-3)
P 		181.4
(-1.1)
P 		262.3
(3)
P 		119
avr 209.5
(-0.2) 		138.1
(-3) 		167
(-1.9) 		290.3
(3) 		152

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 196
1 2 3 4 5 6 range
A 183.1
(-2.5)
P 		173.5
(-3)
P 		183.9
(-2.4)
P 		192.5
(-1.9)
P 		280.4
(2.9)
P 		282
(3)
P 		109
B 268.2
(-0.9)
P 		290.6
(-0)
P 		212.8
(-3)
P 		220.7
(-2.7)
P 		258.6
(-1.2)
P 		369.3
(3)
P 		157
C 243.1
(2.4)
P 		248.6
(3)
P 		196.2
(-3)
P 		230.4
(0.9)
P 		203.7
(-2.1)
P 		234.9
(1.4)
P 		52
avr 231.5
(-0.9) 		237.6
(-0.5) 		197.6
(-3) 		214.5
(-2) 		247.6
(0.1) 		295.4
(3) 		98
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 60
1 2 3 4 range
A 89.3
(3)
P 		30.5
(-3)
P 		50.6
(-0.9)
P 		36.8
(-2.4)
P 		59
B 61.8
(1.2)
P 		32.2
(-2.6)
P 		29.2
(-3)
P 		76
(3)
P 		47
C 62.7
(2.9)
P 		41.2
(-3)
P 		63.2
(3)
P 		44.2
(-2.2)
P 		22
avr 71.3
(3) 		34.6
(-3) 		47.7
(-0.9) 		52.3
(-0.1) 		37

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 104
1 2 3 4 5 6 range
A 33.7
(-3)
P 		61.7
(0.3)
P 		40
(-2.3)
P 		48.8
(-1.2)
P 		84.6
(3)
P 		68.7
(1.1)
P 		51
B 93.3
(-0.1)
P 		96.7
(0.1)
P 		51.7
(-3)
P 		58.5
(-2.5)
P 		105.1
(0.7)
P 		137.7
(3)
P 		86
C 90.1
(3)
P 		75.8
(1)
P 		49.9
(-2.5)
P 		46.3
(-3)
P 		65.9
(-0.3)
P 		78.6
(1.4)
P 		44
avr 72.4
(0.2) 		78.1
(0.9) 		47.2
(-3) 		51.2
(-2.5) 		85.2
(1.8) 		95
(3) 		48
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187466_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7758
Sequence Type Exemplar sequence
Representative Public ID B0303.4 NCBI
Target Description B0303.4 /REP_DB=WormBase Gene ID /WP=CE00537 /TR=SW:P34256 /GB=AAA27898.2 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066515(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066515 NCBI Caenorhabditis elegans B0303.4 (B0303.4) mRNA, complete cds. 9/11 None
B0303.4.1 ENSEMBL cdna:known chromosome:CEL140:III:8689752:8693354:1 gene:B0303.4 9/11 None
B0303.4.2 ENSEMBL cdna:known chromosome:CEL140:III:8689844:8693354:1 gene:B0303.4 9/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000042404 8/11 Cross Hyb Matching Probes None
GENEFINDER00000042424 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8689857-8693338(+) 95.6 100.0
Public Domain and Genome References
Gene Title
Ensembl B0303.4
Entrez Gene 176218 Entrez gene
SwissProt P34256 EMBL-EBI
WormBase CE29540 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA27898 Hypothetical protein B0303.4 [Caenorhabditis elegans] ref|NP_498916.1| B0303.4 [Caenorhabditis elegans] sp|P34256|YKA4_CAEEL Hypothetical protein B0303.4 in chromosome III 0.0
blast D88536 acidic protein - Caenorhabditis elegans 0.0
blast CAE62726 Hypothetical protein CBG06886 [Caenorhabditis briggsae] 1.0E-102
Protein Domains
Database ID Description E-Value
Pfam IPR000626 EMBL-EBI Ubiquitin 1.3E-14
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_498916.1 4 12-34,475-497,544-563,568-587
B0303.4.1 4 12-34,475-497,544-563,568-587
B0303.4.2 4 12-34,475-497,544-563,568-587
Sequence
Target Sequence 
>C. ELEGANS:187466_AT
cttctgacgattagccaatgggtatgcaatttacttgttgataatggattgttggaacaa
gatgatgatgatcaggaaactcatttgcaaacttcaacattattttggatttttggaggt
gattctaattttcaaaaaaaaacacgtcaaaacgcaatatttattacaggtcaaatggtc
gcagtgagcatatttctgtactactttccagatgtatttgatcgagtcggcttttcgatc
ttcattattgtatttctttacttcgtttttgtggtttattcacgtcagcgacgacgccaa
ccggatcctgttgaggttcaaaatgaaatgatggaaaatcaaatcgtccaggagactatt
cgtcttctattcttgtacacttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTTCTGACGATTAGCCAATGGGTAT 300 197 1543 Antisense
GGAAACTCATTTGCAAACTTCAACA 562 527 1617 Antisense
CAGGTCAAATGGTCGCAGTGAGCAT 538 207 1709 Antisense
GTGAGCATATTTCTGTACTACTTTC 389 483 1726 Antisense
TGTACTACTTTCCAGATGTATTTGA 28 559 1739 Antisense
TGTATTTGATCGAGTCGGCTTTTCG 428 559 1755 Antisense
GAGTCGGCTTTTCGATCTTCATTAT 154 401 1766 Antisense
TATTCACGTCAGCGACGACGCCAAC 675 655 1819 Antisense
ACGCCAACCGGATCCTGTTGAGGTT 195 91 1836 Antisense
GACTATTCGTCTTCTATTCTTGTAC 303 373 1896 Antisense
TTCGTCTTCTATTCTTGTACACTTC 42 697 1901 Antisense
Sequence Source Affymetrix Proprietary Database