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[187463_at]

Description C08B11.8 /REP_DB=WormBase Gene ID /WP=CE01478 /TR=SW:Q09226 /GB=CAA86666.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 59      6grp_high1_10_10 > 94      4grp_highest_8_8 > 17      4grp_high_8_8 > 61      6grp_highest_nonorm > 74      6grp_total > 85      4grp_total > 59      6grp_highest_sub > In      6grp_high1_sub > In      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 317
1 2 3 4 range
A 566.2
(-0.4)
P 		532.8
(-1)
P 		744.7
(3)
P 		427.7
(-3)
P 		317
B 607.4
(0.3)
P 		715
(3)
P 		638.7
(1.1)
P 		472.2
(-3)
P 		243
C 605.5
(0.4)
P 		668.3
(1.7)
P 		732.3
(3)
P 		443.5
(-3)
P 		289
avr 593
(0.4) 		638.7
(1.4) 		705.2
(3) 		447.8
(-3) 		257

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 472
1 2 3 4 5 6 range
A 590.8
(-2.1)
P 		558
(-3)
P 		648.8
(-0.5)
P 		655.1
(-0.4)
P 		778.1
(3)
P 		735.2
(1.8)
P 		220
B 575.4
(-3)
P 		606.7
(-2.4)
P 		587.1
(-2.8)
P 		774
(0.6)
P 		693.1
(-0.9)
P 		904.7
(3)
P 		329
C 433
(-3)
P 		708.4
(1.1)
P 		681.9
(0.7)
P 		834.1
(3)
P 		569.4
(-1)
P 		656.6
(0.3)
P 		401
avr 533.1
(-3) 		624.4
(-0.6) 		639.3
(-0.3) 		754.4
(2.7) 		680.2
(0.8) 		765.5
(3) 		232
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 193
1 2 3 4 range
A 206.2
(3)
P 		115.7
(-0.8)
P 		194.7
(2.5)
P 		62.6
(-3)
P 		144
B 193.7
(3)
P 		176.6
(1.9)
P 		148
(0.1)
P 		100.6
(-3)
P 		93
C 200.4
(1.2)
P 		192.1
(0.9)
P 		255.2
(3)
P 		74.8
(-3)
P 		180
avr 200.1
(3) 		161.5
(1.1) 		199.3
(3) 		79.3
(-3) 		121

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 229
1 2 3 4 5 6 range
A 108.9
(-3)
P 		198.4
(1.3)
P 		141.3
(-1.5)
P 		166.2
(-0.3)
P 		234.8
(3)
P 		179
(0.3)
P 		126
B 200.2
(-1.2)
P 		202
(-1.2)
P 		142.6
(-3)
P 		205
(-1.1)
P 		281.6
(1.3)
P 		337.4
(3)
P 		195
C 160.5
(-3)
P 		216.2
(2.6)
P 		173.6
(-1.7)
P 		167.7
(-2.3)
P 		184.3
(-0.6)
P 		219.8
(3)
P 		59
avr 156.5
(-2.7) 		205.5
(0.4) 		152.5
(-3) 		179.6
(-1.2) 		233.6
(2.2) 		245.4
(3) 		93
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187463_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3855
Sequence Type Exemplar sequence
Representative Public ID C08B11.8 NCBI
Target Description C08B11.8 /REP_DB=WormBase Gene ID /WP=CE01478 /TR=SW:Q09226 /GB=CAA86666.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063284(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063284 NCBI Caenorhabditis elegans C08B11.8 (C08B11.8) mRNA, complete cds. 11/11 None
C08B11.8 ENSEMBL cdna:known chromosome:CEL140:II:8039931:8041638:1 gene:C08B11.8 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000039310 1/11 Cross Hyb Matching Probes None
GENEFINDER00000039317 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:8039927-8041635(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C08B11.8
Entrez Gene 182392 Entrez gene
SwissProt Q09226 EMBL-EBI
WormBase CE01478 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:249513_AT ALG6, ALG8 glycosyltransferase family protein at
CANINE_2:CFAAFFX.28581.1.S1_AT similar to Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase (Dolichyl-P-Glc:Man9GlcNAc2-PP-dolichyl glucosyltransferase) cfa
DROSOPHILA_2:1636630_S_AT dm
DROSOPHILA_2:1623305_AT dm
DROSGENOME1:142765_AT dm
CHICKEN:GGA.5118.1.S1_S_AT asparagine-linked glycosylation 6 homolog (yeast, alpha-1,3-glucosyltransferase) gga
HG-U133_PLUS_2:219649_AT asparagine-linked glycosylation 6 homolog (yeast, alpha-1,3-glucosyltransferase) hs
HG-U133A:219649_AT asparagine-linked glycosylation 6 homolog (yeast, alpha-1,3-glucosyltransferase) hs
HG-FOCUS:219649_AT asparagine-linked glycosylation 6 homolog (yeast, alpha-1,3-glucosyltransferase) hs
HG-U133A_2:219649_AT asparagine-linked glycosylation 6 homolog (yeast, alpha-1,3-glucosyltransferase) hs
HU35KSUBB:RC_N63143_AT asparagine-linked glycosylation 6 homolog (yeast, alpha-1,3-glucosyltransferase) hs
U133_X3P:G7019324_3P_AT asparagine-linked glycosylation 6 homolog (yeast, alpha-1,3-glucosyltransferase) hs
HG-U95B:52733_AT asparagine-linked glycosylation 6 homolog (yeast, alpha-1,3-glucosyltransferase) hs
HG-U95B:52735_G_AT asparagine-linked glycosylation 6 homolog (yeast, alpha-1,3-glucosyltransferase) hs
HG-U95E:88210_AT asparagine-linked glycosylation 6 homolog (yeast, alpha-1,3-glucosyltransferase) hs
HU35KSUBC:RC_D20155_AT Asparagine-linked glycosylation 6 homolog (yeast, alpha-1,3-glucosyltransferase) hs
MG-U74BV2:114103_F_AT asparagine-linked glycosylation 6 homolog (yeast, alpha-1,3,-glucosyltransferase) mm
MG-U74BV2:114104_R_AT asparagine-linked glycosylation 6 homolog (yeast, alpha-1,3,-glucosyltransferase) mm
MG-U74BV2:164349_R_AT asparagine-linked glycosylation 6 homolog (yeast, alpha-1,3,-glucosyltransferase) mm
MOE430B:1439007_AT asparagine-linked glycosylation 6 homolog (yeast, alpha-1,3,-glucosyltransferase) mm
MOUSE430_2:1439007_AT asparagine-linked glycosylation 6 homolog (yeast, alpha-1,3,-glucosyltransferase) mm
MU19KSUBA:TC16906_AT PREDICTED: Mus musculus asparagine-linked glycosylation 6 homolog (yeast, alpha-1,3,-glucosyltransferase) (Alg6), mRNA mm
YEAST_2:1773141_AT Glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease Sc
Protein Similarities
Method ID Description E-Value
blast CAA86666 Hypothetical protein C08B11.8 [Caenorhabditis elegans] ref|NP_495685.1| C08B11.8 [Caenorhabditis elegans] sp|Q09226|ALG6_CAEEL Probable dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase (Dolichyl-P-Glc:Man9GlcNAc2-PP-dolichyl glucosyltransferase) 0.0
blast CAE67770 Hypothetical protein CBG13345 [Caenorhabditis briggsae] 0.0
blast XP_796240 PREDICTED: similar to dolichyl-P-Glc:Man9GlcNAc2-PP- dolichylglucosyltransferase, partial [Strongylocentrotus purpuratus] 8.0E-88
Protein Domains
Database ID Description E-Value
Pfam IPR004856 EMBL-EBI ALG6, ALG8 glycosyltransferase 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_495685.1 11 47-64,152-174,181-198,213-235,269-291,331-353,360-379,383-405,412-434,444-463,475-497
C08B11.8 11 47-64,152-174,181-198,213-235,269-291,331-353,360-379,383-405,412-434,444-463,475-497
Sequence
Target Sequence 
>C. ELEGANS:187463_AT
taattcgcgtttttccattcaatcgtggactatatgaagataaagtagcaagtttctggt
gtgcgttctcgtttatactaaaacgcctaccgcttcagagcgtgcaaatttatatcagca
ctgccctcgtacttgctggatctgcaccgtctctgctagtattgttcttacgacccacag
aaaaacaattcagaatttcgttaacagcgacaggactttcctttttcttattttccttcc
acgtacatgaaaagactatccttctagctgcagttccagcgcttttgctaatttctgaat
atacatctcttgtcatttggttcctgaacataacaaatataagcattttcagtttgtgcg
tcaaggataattttgccctttcactctctttcttcttcgcatattttgtt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TAATTCGCGTTTTTCCATTCAATCG 188 633 902 Antisense
TTCTGGTGTGCGTTCTCGTTTATAC 581 693 955 Antisense
TATACTAAAACGCCTACCGCTTCAG 664 647 975 Antisense
AATTTATATCAGCACTGCCCTCGTA 408 175 1008 Antisense
GCACCGTCTCTGCTAGTATTGTTCT 417 315 1045 Antisense
GCGACAGGACTTTCCTTTTTCTTAT 367 287 1108 Antisense
AAAGACTATCCTTCTAGCTGCAGTT 670 121 1152 Antisense
TGCAGTTCCAGCGCTTTTGCTAATT 515 577 1170 Antisense
TACATCTCTTGTCATTTGGTTCCTG 168 645 1203 Antisense
AGGATAATTTTGCCCTTTCACTCTC 196 57 1265 Antisense
CTCTTTCTTCTTCGCATATTTTGTT 125 231 1287 Antisense
Sequence Source Affymetrix Proprietary Database