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[187461_at]

Description Y104H12D.2 /REP_DB=WormBase Gene ID /WP=CE24050 /TR=Q9N5A2 /GB=AAF60395.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 59      6grp_high1_10_10 > 94      4grp_highest_8_8 > 38      4grp_high_8_8 > 03      6grp_highest_nonorm > 82      6grp_total > 75      4grp_total > 13      6grp_highest_sub > In      6grp_high1_sub > In      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 206
1 2 3 4 range
A 331.7
(3)
P 		237.6
(-2.8)
P 		234.9
(-3)
P 		311.8
(1.8)
P 		97
B 288.7
(-1.1)
P 		269
(-2.8)
P 		267.1
(-3)
P 		334.3
(3)
P 		67
C 272.1
(-1.6)
P 		222.7
(-3)
P 		223.2
(-3)
P 		428.8
(3)
P 		206
avr 297.5
(-0.1) 		243.1
(-2.9) 		241.7
(-3) 		358.3
(3) 		117

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 254
1 2 3 4 5 6 range
A 327.8
(0.6)
P 		231.3
(-3)
P 		323.5
(0.5)
P 		322
(0.4)
P 		391.5
(3)
P 		291.5
(-0.7)
P 		160
B 275.1
(-3)
P 		340.3
(-0.8)
P 		303.6
(-2)
P 		332.9
(-1)
P 		312.4
(-1.7)
P 		452.7
(3)
P 		178
C 199.2
(-3)
P 		276.2
(-1)
P 		318.1
(0.2)
P 		424.6
(3)
P 		268.3
(-1.2)
P 		383.7
(1.9)
P 		225
avr 267.4
(-3) 		282.6
(-2.2) 		315.1
(-0.4) 		359.8
(2.1) 		324.1
(0.1) 		376
(3) 		109
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 75
1 2 3 4 range
A 120.8
(3)
P 		51.6
(-2.5)
P 		61.4
(-1.7)
P 		45.6
(-3)
P 		75
B 92.1
(3)
P 		66.4
(-2.1)
P 		61.9
(-3)
P 		71.2
(-1.2)
P 		30
C 90.1
(3)
P 		64
(-3)
P 		77.8
(0.2)
P 		72.3
(-1.1)
P 		26
avr 101
(3) 		60.7
(-3) 		67
(-2.1) 		63
(-2.7) 		40

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 108
1 2 3 4 5 6 range
A 60.4
(-3)
P 		82.3
(-0.7)
P 		70.4
(-2)
P 		81.7
(-0.8)
P 		118.1
(3)
P 		71
(-1.9)
P 		58
B 95.7
(-1.6)
P 		113.3
(-0.5)
P 		73.7
(-3)
P 		88.2
(-2.1)
P 		126.9
(0.4)
P 		168.8
(3)
P 		95
C 73.8
(-3)
P 		84.3
(-1.8)
P 		81
(-2.2)
P 		85.4
(-1.7)
P 		86.8
(-1.6)
P 		128.4
(3)
P 		55
avr 76.6
(-2.8) 		93.3
(-0.7) 		75
(-3) 		85.1
(-1.7) 		110.6
(1.5) 		122.7
(3) 		48
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187461_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11164
Sequence Type Exemplar sequence
Representative Public ID Y104H12D.2 NCBI
Target Description Y104H12D.2 /REP_DB=WormBase Gene ID /WP=CE24050 /TR=Q9N5A2 /GB=AAF60395.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171253, NM_067648
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
SNAP00000032194 ENSEMBL cdna:SNAP chromosome:CEL140:IV:1178039:1182790:1 None
NM_067648 NCBI Caenorhabditis elegans Y104H12D.2 (Y104H12D.2) mRNA, complete cds. A 
SNAP00000032195 ENSEMBL cdna:SNAP chromosome:CEL140:IV:1182975:1201418:1 None
NM_171253 NCBI Caenorhabditis elegans Y104H12D.4 (Y104H12D.4) mRNA, complete cds. None
Y104H12D.2 ENSEMBL cdna:known chromosome:CEL140:IV:1178022:1182837:1 gene:Y104H12D.2 A 
Y104H12D.4 ENSEMBL cdna:known chromosome:CEL140:IV:1182592:1186604:1 gene:Y104H12D.4 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000032198 4/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:1181188-1185046(+) 100.0 100.0
Public Domain and Genome References
Gene Title

Ensembl Y104H12D.2
Y104H12D.4
Entrez Gene 176936 Entrez gene
3564762 Entrez gene
SwissProt Q9N5A2 EMBL-EBI
Q8MXU4 EMBL-EBI
WormBase CE31631 Wormbase
CE31633 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK68403 Hypothetical protein Y104H12D.3 [Caenorhabditis elegans] ref|NP_500051.2| Y104H12D.3 [Caenorhabditis elegans] 0.0
blast AAM97962 Hypothetical protein Y104H12D.2 [Caenorhabditis elegans] ref|NP_500049.2| Y104H12D.2 [Caenorhabditis elegans] 1.0E-175
blast CAE65555 Hypothetical protein CBG10544 [Caenorhabditis briggsae] 2.0E-96
blast AAC69001 Sphingosine phosphate lyase protein 1 [Caenorhabditis elegans] ref|NP_499913.1| Sphingosine Phosphate Lyase family member (spl-1) [Caenorhabditis elegans] gb|AAD44756.1| sphingosine-1-phosphate lyase [Caenorhabditis elegans] sp|Q9Y194|SGPL_CAEEL Sphingosine-1-phosphate lyase (SP-lyase) (SPL) (Sphingosine-1-phosphate aldolase) 6.0E-96
blast T32508 hypothetical protein C50A2.1 - Caenorhabditis elegans 7.0E-89
blast AAM97963 Hypothetical protein Y104H12D.4 [Caenorhabditis elegans] ref|NP_741302.1| Y104H12D.4 [Caenorhabditis elegans] 2.0E-57
Protein Domains
Database ID Description E-Value
Pfam IPR002129 EMBL-EBI Pyridoxal-dependent decarboxylase 0.48
Pfam IPR002129 EMBL-EBI Pyridoxal-dependent decarboxylase 2.9E-5
Trans Membrane
ID Number Of Domains Domain Boundaries
SNAP00000032195 1 245-264
Sequence
Target Sequence 
>C. ELEGANS:187461_AT
agcagatgatagtccttcggctcgagcactaaatgacgtcatatcaatcgataatcacgt
tatgttcaaagctatgctaatcaatggaagatatgacgctgttgaatggaaaaaaattgc
cgattctcatgaacttgtgatgccgaaaaaagtgaacacgtcggattcgtatgcacagac
tcctgcgtgtcttgtgacaatcatgttccgaaaactgctccaaaatcatccagaagatat
ggatcgatactcatcaatattggatcaattgccaccgtcagcacttccagattgctattt
ttcccgcacctacgagctgaaatgcccatttgcggaaggtttcacaatggaaattggcga
tttgttcctttttcactttgttttcgatggctcctcggagaaatgcacacgtttgacgcc
gatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGCAGATGATAGTCCTTCGGCTCGA 121 77 171 Antisense
TCGGCTCGAGCACTAAATGACGTCA 488 605 187 Antisense
GCCGATTCTCATGAACTTGTGATGC 604 283 289 Antisense
GTCGGATTCGTATGCACAGACTCCT 629 471 330 Antisense
CACAGACTCCTGCGTGTCTTGTGAC 620 195 344 Antisense
CACTTCCAGATTGCTATTTTTCCCG 374 195 452 Antisense
TTCCCGCACCTACGAGCTGAAATGC 162 699 471 Antisense
AATGCCCATTTGCGGAAGGTTTCAC 245 161 491 Antisense
TTCCTTTTTCACTTTGTTTTCGATG 10 699 535 Antisense
TTGTTTTCGATGGCTCCTCGGAGAA 155 707 548 Antisense
GAAATGCACACGTTTGACGCCGATT 477 361 570 Antisense
Sequence Source Affymetrix Proprietary Database