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[187458_at]

Description Y54E10BL.4 /REP_DB=WormBase Gene ID /WP=CE25436 /TR=Q9N3E0 /GB=AAF60776.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 88      4grp_total > 59      6grp_cgc > 59      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 35
1 2 3 4 range
A 3.1
(-3)
A
34.1
(2.3)
A
38
(3)
A
7.7
(-2.2)
A
35
B 23.6
(0.5)
A
27.3
(3)
A
26.4
(2.4)
A
18.3
(-3)
A
9
C 27
(3)
A
4.6
(-3)
A
9.5
(-1.7)
A
6
(-2.6)
A
22
avr 17.9
(0.1)
22
(1.9)
24.6
(3)
10.7
(-3)
14

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 81
1 2 3 4 5 6 range
A 34.9
(-2.1)
A
30.9
(-2.5)
A
32.7
(-2.3)
A
26.6
(-3)
A
46
(-0.9)
P
82.6
(3)
P
56
B 32.6
(-2.7)
A
35.8
(-2.4)
A
28.7
(-3)
A
33.7
(-2.6)
A
41.3
(-1.9)
P
98.3
(3)
P
70
C 32.7
(-2.4)
A
24.5
(-3)
A
52.8
(-0.9)
A
37.9
(-2)
A
98.8
(2.5)
P
105.6
(3)
P
81
avr 33.4
(-2.7)
30.4
(-3)
38.1
(-2.3)
32.7
(-2.8)
62
(-0.1)
95.5
(3)
65
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9
1 2 3 4 range
A 1.1
(-3)
A
7.4
(1.3)
A
9.9
(3)
A
1.1
(-3)
A
9
B 7.5
(3)
A
6.7
(1.7)
A
6.1
(0.7)
A
3.9
(-3)
A
4
C 8.9
(3)
A
1.3
(-2.8)
A
3.3
(-1.3)
A
1
(-3)
A
8
avr 5.8
(2.2)
5.1
(1.2)
6.4
(3)
2
(-3)
4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 30
1 2 3 4 5 6 range
A 6.4
(-3)
A
11
(-1)
A
7.1
(-2.7)
A
6.7
(-2.9)
A
13.9
(0.3)
P
20.1
(3)
P
14
B 11.4
(-2.1)
A
11.9
(-2)
A
7
(-3)
A
8.9
(-2.6)
A
16.8
(-1)
P
36.7
(3)
P
30
C 12.1
(-2)
A
7.5
(-3)
A
13.4
(-1.7)
A
7.6
(-3)
A
32
(2.3)
P
35.4
(3)
P
28
avr 10
(-2.4)
10.1
(-2.4)
9.2
(-2.6)
7.7
(-3)
20.9
(0.4)
30.7
(3)
23
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187458_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.177
Sequence Type Exemplar sequence
Representative Public ID Y54E10BL.4 NCBI
Target Description Y54E10BL.4 /REP_DB=WormBase Gene ID /WP=CE25436 /TR=Q9N3E0 /GB=AAF60776.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058683(11)
Transcript Assignments
Representative
Transcript
Description Matching Probes Related Probesets by Grade
NM_058683 NCBI Caenorhabditis elegans DNaJ domain (prokaryotic heat shock protein) family member (dnj-28) (dnj-28) mRNA, complete cds. 11/11 None
SNAP00000040892 ENSEMBL cdna:SNAP chromosome:CEL140:I:2996511:3001267:1 11/11 None
GENEFINDER00000040900 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:2996511:3001243:1 11/11 None
Y54E10BL.4 ENSEMBL cdna:known chromosome:CEL140:I:2996407:3001299:1 gene:Y54E10BL.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:2996510-3001267(+) 97.51 100.0
Public Domain and Genome References
Gene Title
Gene Symbol dnj-28
Ensembl Y54E10BL.4
Entrez Gene 171870 Entrez gene
SwissProt Q9N3E0 EMBL-EBI
WormBase CE25436 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF60776 Dnaj domain (prokaryotic heat shock protein) protein 28 [Caenorhabditis elegans] ref|NP_491084.1| DNaJ domain (prokaryotic heat shock protein) family member (dnj-28) [Caenorhabditis elegans] 0.0
blast CAE60558 Hypothetical protein CBG04187 [Caenorhabditis briggsae] 1.0E-168
blast CAE65855 Hypothetical protein CBG10993 [Caenorhabditis briggsae] 1.0E-122
Protein Domains
Database ID Description E-Value
scop a.2.3.Chaperone J-domain All alpha proteins; Long alpha-hairpin; Chaperone J-domain; Chaperone J-domain 1.99999996755032E-17
scop a.2.3.Chaperone J-domain All alpha proteins; Long alpha-hairpin; Chaperone J-domain; Chaperone J-domain 9.99999983775159E-18
Pfam IPR001623 EMBL-EBI Heat shock protein DnaJ, N-terminal 2.8E-29
Pfam IPR001440 EMBL-EBI TPR repeat 1.3E-6
Pfam IPR001440 EMBL-EBI TPR repeat 0.34
Pfam IPR001440 EMBL-EBI TPR repeat 0.24
Pfam IPR001440 EMBL-EBI TPR repeat 0.16
Pfam IPR001440 EMBL-EBI TPR repeat 7.3E-6
Sequence
Target Sequence
>C. ELEGANS:187458_AT
gaagccattcaacagtgtactcgagtactggaatttgatgattctgatgtggaaacgttg
attcagagagctgaagcttatatggcagacgaggagtatgatatggcaatcgctgactac
gaaaaagccgaagaatgggattcatcaaacgacgccgtccgaactggaaaagatcaagca
aagcgtgcaaaggaactcgtcggaaaacgagattactacaaaattctcggagtccgccgg
aacgccaataaacgagaaatcacaaaagcctatcgaaaaatggctcaaaaatggcatccg
gacaactttcaggatgagaaggagaagaagaaagctgagaagaagtttatcgatattgcc
gcggcgaaggaagttttgagcaatgaggagaagcggcgagctttcgataatggacaagat
ccactggattcggaagcagggaggggtggaggtggaggtggaggatcacacggatttcat
aatttccacggatttaatccattcggcggtggtggtgg


BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position
Strandedness
GAAGCCATTCAACAGTGTACTCGAG 185 345 940 Antisense
GTATGATATGGCAATCGCTGACTAC 704 453 1035 Antisense
ATCAAACGACGCCGTCCGAACTGGA 12 25 1083 Antisense
AAGCGTGCAAAGGAACTCGTCGGAA 107 149 1120 Antisense
TACAAAATTCTCGGAGTCCGCCGGA 77 641 1156 Antisense
TCCGCCGGAACGCCAATAAACGAGA 340 595 1172 Antisense
AGAAGTTTATCGATATTGCCGCGGC 247 75 1280 Antisense
GCCGCGGCGAAGGAAGTTTTGAGCA 319 285 1297 Antisense
AAGCGGCGAGCTTTCGATAATGGAC 646 149 1330 Antisense
GATCCACTGGATTCGGAAGCAGGGA 328 415 1357 Antisense
TTAATCCATTCGGCGGTGGTGGTGG 156 683 1433 Antisense
Sequence Source Affymetrix Proprietary Database