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[187452_at]

Description C31H2.4 /REP_DB=WormBase Gene ID /WP=CE04132 /TR=SW:Q18347 /GB=AAA83338.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 46      6grp_high1_10_10 > 54      4grp_highest_8_8 > 09      4grp_high_8_8 > 61      6grp_highest_nonorm > 72      6grp_total > 54      4grp_total > 58      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 297
1 2 3 4 range
A 331.9
(-0.1)
P 		402.4
(1.5)
P 		465.8
(3)
P 		210.5
(-3)
P 		255
B 283.4
(-0.8)
P 		465
(3)
P 		395.8
(1.5)
P 		179.2
(-3)
P 		286
C 401
(0.4)
P 		433.4
(1.5)
P 		476.4
(3)
P 		303.2
(-3)
P 		173
avr 338.8
(0) 		433.6
(2.7) 		446
(3) 		231
(-3) 		215

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 253
1 2 3 4 5 6 range
A 275.1
(-3)
P 		376.7
(0.4)
P 		409.7
(1.6)
P 		347.1
(-0.6)
P 		276.6
(-2.9)
P 		451.8
(3)
P 		177
B 395
(-0.3)
P 		367.5
(-1)
P 		528.2
(3)
P 		333
(-1.9)
P 		289.1
(-3)
P 		380.2
(-0.7)
P 		239
C 401.9
(1)
P 		370.4
(-0.2)
P 		456.9
(3)
P 		390.6
(0.5)
P 		294.8
(-3)
P 		352.7
(-0.9)
P 		162
avr 357.3
(-0.6) 		371.5
(-0.1) 		464.9
(3) 		356.9
(-0.6) 		286.8
(-3) 		394.9
(0.6) 		178
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 135
1 2 3 4 range
A 120.9
(2.9)
P 		87.4
(0.7)
P 		121.8
(3)
P 		30.8
(-3)
P 		91
B 90.4
(1.1)
P 		114.9
(3)
P 		91.7
(1.2)
P 		38.2
(-3)
P 		77
C 132.8
(1.3)
P 		124.6
(0.8)
P 		166
(3)
P 		51.1
(-3)
P 		115
avr 114.7
(2.2) 		109
(1.8) 		126.5
(3) 		40
(-3) 		87

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 98
1 2 3 4 5 6 range
A 50.7
(-3)
P 		134
(3)
P 		89.2
(-0.2)
P 		88
(-0.3)
P 		83.5
(-0.6)
P 		110
(1.3)
P 		83
B 137.4
(2.5)
P 		122.3
(0.8)
P 		128.3
(1.5)
P 		88.2
(-3)
P 		117.5
(0.3)
P 		141.8
(3)
P 		54
C 149
(3)
P 		113
(-0.1)
P 		116.3
(0.2)
P 		78.6
(-3)
P 		95.4
(-1.6)
P 		118.1
(0.4)
P 		70
avr 112.4
(1.3) 		123.1
(3) 		111.3
(1.1) 		84.9
(-3) 		98.8
(-0.8) 		123.3
(3) 		38
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187452_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16261
Sequence Type Exemplar sequence
Representative Public ID C31H2.4 NCBI
Target Description C31H2.4 /REP_DB=WormBase Gene ID /WP=CE04132 /TR=SW:Q18347 /GB=AAA83338.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076474(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076474 NCBI Caenorhabditis elegans C31H2.4 (C31H2.4) mRNA, complete cds. 11/11 None
SNAP00000008497 ENSEMBL cdna:SNAP chromosome:CEL140:X:5155131:5159314:-1 11/11 None
GENEFINDER00000008499 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:5155131:5159628:-1 11/11 None
C31H2.4 ENSEMBL cdna:known chromosome:CEL140:X:5155131:5159628:-1 gene:C31H2.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:5155131-5159629(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C31H2.4
Entrez Gene 183101 Entrez gene
SwissProt Q18347 EMBL-EBI
WormBase CE04132 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
9072 aromatic amino acid family metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3868 4-hydroxyphenylpyruvate dioxygenase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA83338 Hypothetical protein C31H2.4 [Caenorhabditis elegans] ref|NP_508875.1| C31H2.4 [Caenorhabditis elegans] sp|Q18347|YBWL_CAEEL Hypothetical protein C31H2.4 in chromosome X 0.0
blast CAE68701 Hypothetical protein CBG14622 [Caenorhabditis briggsae] 0.0
blast AAA83338 Hypothetical protein C31H2.4 [Caenorhabditis elegans] ref|NP_508875.1| C31H2.4 [Caenorhabditis elegans] sp|Q18347|YBWL_CAEEL Hypothetical protein C31H2.4 in chromosome X 1.0E-177
blast CAE68701 Hypothetical protein CBG14622 [Caenorhabditis briggsae] 1.0E-157
blast CAA90315 Hypothetical protein T21C12.2 [Caenorhabditis elegans] ref|NP_499324.1| 4-HydroxyPhenylpyruvate Dioxygenase (HPD) family member (hpd-1) [Caenorhabditis elegans] sp|Q22633|HPPD_CAEEL 4-hydroxyphenylpyruvate dioxygenase (4HPPD) (HPD) (HPPDase) 2.0E-86
Protein Domains
Database ID Description E-Value
Pfam IPR004360 EMBL-EBI Glyoxalase/Bleomycin resistance protein/dioxygenase domain 3.2E-10
Pfam IPR004360 EMBL-EBI Glyoxalase/Bleomycin resistance protein/dioxygenase domain 3.2E-10
Sequence
Target Sequence 
>C. ELEGANS:187452_AT
aaaatgcccgcaatccttatggatcatgtagttcaaaactatccaattggggagatggaa
gcagccgccgattggtattttaagacaatgaggcttaaaaggttttggtccgtagacgat
aaagttgcaacatcagaattttctgccatgaccgcttggctgttagtaaatgatgatcat
actgtacaggttacattagcagaaggagtaaagggacggaagggaaaaagtcaaattgag
gaattcattaattatcatggtggctcaggagttcaacattttgctttattagttgaagat
attatatctgcagtccaaataatgaaaagcagaagcgtggagtttctcacaatccctagc
caatattatgacaatttggaagaaaggctaagcaaaacaaatttgatagtcaaggaagac
ttaaaaatgattcgcgaattgaacattctcatggactttgacgagaacggttatcttctc
caaattttcagcaagccattgcaagatagacccacgctcttcatcgagattatccagcgg
gcgaatttcaaggggtttggagctggaaatttcaaagcgctgttcgacgcagtgga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAATGCCCGCAATCCTTATGGATC 578 133 478 Antisense
GAAGCAGCCGCCGATTGGTATTTTA 447 341 535 Antisense
GAATTTTCTGCCATGACCGCTTGGC 147 325 613 Antisense
GTGGCTCAGGAGTTCAACATTTTGC 485 491 737 Antisense
GAAGCGTGGAGTTTCTCACAATCCC 603 341 809 Antisense
TTCTCACAATCCCTAGCCAATATTA 102 691 821 Antisense
GTTATCTTCTCCAAATTTTCAGCAA 584 447 947 Antisense
GCCATTGCAAGATAGACCCACGCTC 500 281 972 Antisense
CACGCTCTTCATCGAGATTATCCAG 663 199 990 Antisense
GATTATCCAGCGGGCGAATTTCAAG 542 429 1005 Antisense
TCAAAGCGCTGTTCGACGCAGTGGA 548 629 1049 Antisense
Sequence Source Affymetrix Proprietary Database