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[187440_at]

Description R12B2.6 /REP_DB=WormBase Gene ID /WP=CE01369 /TR=SW:P45898 /GB=AAA50744.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 74      4grp_total > 36      6grp_cgc > 66      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 101
1 2 3 4 range
A 70.7
(3)
A 		28.8
(-0.8)
A 		4.8
(-3)
A 		66.5
(2.6)
A 		66
B 49.6
(-2.3)
A 		106.2
(3)
A 		59
(-1.4)
A 		42.3
(-3)
A 		64
C 42.2
(-3)
A 		59.5
(3)
A 		43.5
(-2.5)
A 		43.1
(-2.7)
A 		17
avr 54.2
(0.8) 		64.8
(3) 		35.8
(-3) 		50.6
(0.1) 		29

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 74
1 2 3 4 5 6 range
A 22.7
(-3)
A 		25.9
(-2.7)
A 		54.3
(0.3)
A 		80.1
(3)
A 		31.3
(-2.1)
A 		42.4
(-0.9)
A 		57
B 39.8
(0.7)
A 		6.3
(-3)
A 		51.8
(2.1)
A 		11.8
(-2.4)
A 		11.9
(-2.4)
A 		60.3
(3)
P 		54
C 10.4
(-3)
A 		30
(-0.9)
A 		41.5
(0.3)
A 		52.1
(1.4)
A 		67.4
(3)
A 		32
(-0.7)
A 		57
avr 24.3
(-2.2) 		20.7
(-3) 		49.2
(3) 		48
(2.7) 		36.9
(0.4) 		44.9
(2.1) 		29
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 25
1 2 3 4 range
A 25.7
(3)
A 		6.3
(-1.8)
A 		1.3
(-3)
A 		9.7
(-0.9)
A 		24
B 15.8
(-0.6)
A 		26.2
(3)
A 		13.7
(-1.4)
A 		9
(-3)
A 		17
C 14
(1.1)
A 		17.1
(3)
A 		15.2
(1.8)
A 		7.3
(-3)
A 		10
avr 18.5
(3) 		16.5
(1.8) 		10.1
(-2.1) 		8.7
(-3) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 20
1 2 3 4 5 6 range
A 4.2
(-3)
A 		9.2
(-1.1)
A 		11.8
(-0.2)
A 		20.3
(3)
A 		9.4
(-1.1)
A 		10.3
(-0.7)
A 		16
B 13.9
(0.5)
A 		2.1
(-3)
A 		12.6
(0.1)
A 		3.1
(-2.7)
A 		4.8
(-2.2)
A 		22.5
(3)
P 		20
C 3.8
(-3)
A 		9.2
(-1.2)
A 		10.6
(-0.7)
A 		10.5
(-0.8)
A 		21.8
(3)
A 		10.7
(-0.7)
A 		18
avr 7.3
(-2.6) 		6.8
(-3) 		11.7
(0.8) 		11.3
(0.5) 		12
(1.1) 		14.5
(3) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187440_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7194
Sequence Type Exemplar sequence
Representative Public ID R12B2.6 NCBI
Target Description R12B2.6 /REP_DB=WormBase Gene ID /WP=CE01369 /TR=SW:P45898 /GB=AAA50744.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171134, NM_065857
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
GENEFINDER00000015250 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:5830172:5835706:-1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_171134 3/11 Cross Hyb Matching Probes None
NM_065857 8/11 Cross Hyb Matching Probes None
SNAP00000015245 6/11 Cross Hyb Matching Probes None
R12B2.5b.1 3/11 Cross Hyb Matching Probes A 
R12B2.5b.2 2/11 Cross Hyb Matching Probes A 
R12B2.5a 3/11 Cross Hyb Matching Probes A 
R12B2.6 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:5833421-5835705(-) 100.0 100.0
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA50743 Mediator protein 15, isoform a [Caenorhabditis elegans] ref|NP_741152.1| MeDiaTor family member (mdt-15) [Caenorhabditis elegans] sp|Q21955|YNL5_CAEEL Hypothetical protein R12B2.5 in chromosome III 0.0
blast AAM54164 Mediator protein 15, isoform b [Caenorhabditis elegans] ref|NP_741151.1| MeDiaTor family member (mdt-15) [Caenorhabditis elegans] 0.0
blast T16726 hypothetical protein R12B2.5 - Caenorhabditis elegans 0.0
Sequence
Target Sequence 
>C. ELEGANS:187440_AT
gaatctcgttgcctgttgctgaggctgctgctgctggtggtcgagctggcaaacatggaa
aagcaatcacattcctcacacctgacgatactgccgtctatttcgacttgaagcaggtaa
aaattgtggacggacaccttccaacacacgtgttctcgttgaattgcctgcgtcgttatg
cccgaatggactcgcggaacatgatgctgcacggagcaaacctagacaattcacatcttg
atgtgaaagtcaaaccggggaacgaatcaaaaagtgattttgccgtcggcgatccgaaaa
ttttgccggctcgtcgactattgacga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATCTCGTTGCCTGTTGCTGAGGC 347 329 17 Antisense
CTGCTGGTGGTCGAGCTGGCAAACA 514 237 47 Antisense
ACCTTCCAACACACGTGTTCTCGTT 414 85 152 Antisense
CGTTGAATTGCCTGCGTCGTTATGC 535 245 173 Antisense
CGTCGTTATGCCCGAATGGACTCGC 67 247 187 Antisense
ATGGACTCGCGGAACATGATGCTGC 177 51 202 Antisense
GATGCTGCACGGAGCAAACCTAGAC 470 413 219 Antisense
AAACCTAGACAATTCACATCTTGAT 21 127 234 Antisense
GTGATTTTGCCGTCGGCGATCCGAA 545 481 290 Antisense
GCGATCCGAAAATTTTGCCGGCTCG 115 289 305 Antisense
TTGCCGGCTCGTCGACTATTGACGA 114 699 319 Antisense
Sequence Source Affymetrix Proprietary Database