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[187436_s_at]

Description F02A9.4B /REP_DB=WormBase Gene ID /WP=CE20652 /TR=SW:P34384 /GB=CAA79619.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 45      6grp_high1_10_10 > 54      4grp_highest_8_8 > 15      4grp_high_8_8 > 06      6grp_highest_nonorm > 74      6grp_total > 55      4grp_total > 10      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 925
1 2 3 4 range
A 898.5
(-2.2)
P 		794.9
(-3)
P 		817.8
(-2.8)
P 		1549.7
(3)
P 		755
B 755.8
(-3)
P 		869.5
(-2.2)
P 		753.5
(-3)
P 		1678.4
(3)
P 		925
C 880.3
(-3)
P 		991.3
(-1.8)
P 		983.8
(-1.9)
P 		1448.2
(3)
P 		568
avr 844.9
(-3) 		885.2
(-2.7) 		851.7
(-2.9) 		1558.8
(3) 		714

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 418
1 2 3 4 5 6 range
A 540.9
(-2.3)
P 		709.2
(1.8)
P 		553.5
(-2)
P 		757.5
(3)
P 		511.7
(-3)
P 		743.3
(2.7)
P 		246
B 579.8
(-1)
P 		698.2
(2.2)
P 		712.7
(2.6)
P 		726.1
(3)
P 		507.4
(-3)
P 		634.7
(0.5)
P 		219
C 772.8
(0.3)
P 		643.6
(-2)
P 		925.4
(3)
P 		589.4
(-3)
P 		628.7
(-2.3)
P 		647.9
(-2)
P 		336
avr 631.2
(-0.3) 		683.7
(1.5) 		730.5
(3) 		691
(1.7) 		549.3
(-3) 		675.3
(1.2) 		181
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 185
1 2 3 4 range
A 327.2
(3)
P 		172.6
(-3)
P 		213.8
(-1.4)
P 		226.8
(-0.9)
P 		155
B 241
(-0.8)
P 		214.8
(-1.7)
P 		174.6
(-3)
P 		357.7
(3)
P 		183
C 291.4
(-0.1)
P 		285
(-0.5)
P 		342.9
(3)
P 		244.2
(-3)
P 		99
avr 286.5
(3) 		224.1
(-3) 		243.8
(-1.1) 		276.2
(2) 		62

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 187
1 2 3 4 5 6 range
A 99.7
(-3)
P 		252.2
(3)
P 		120.6
(-2.2)
P 		192.1
(0.6)
P 		154.4
(-0.8)
P 		181
(0.2)
P 		153
B 201.7
(-0.3)
P 		232.4
(2.6)
P 		173.1
(-3)
P 		192.3
(-1.2)
P 		206.1
(0.1)
P 		236.7
(3)
P 		64
C 286.4
(3)
P 		196.4
(-0.2)
P 		235.5
(1.2)
P 		118.5
(-3)
P 		203.4
(0)
P 		216.9
(0.5)
P 		168
avr 195.9
(-0.1) 		227
(3) 		176.4
(-2.1) 		167.6
(-3) 		188
(-0.9) 		211.5
(1.4) 		59
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 187436_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6374
Sequence Type Exemplar sequence
Representative Public ID F02A9.4B NCBI
Target Description F02A9.4B /REP_DB=WormBase Gene ID /WP=CE20652 /TR=SW:P34384 /GB=CAA79619.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066611(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066611 NCBI Caenorhabditis elegans F02A9.4b (F02A9.4) mRNA, complete cds. 9/11 None
SNAP00000002185 ENSEMBL cdna:SNAP chromosome:CEL140:III:9084794:9089279:-1 10/11 None
GENEFINDER00000002191 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:9084794:9089279:-1 9/11 None
F02A9.4b ENSEMBL cdna:known chromosome:CEL140:III:9084269:9089281:-1 gene:F02A9.4 9/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
176285 2 1 NM_066611 174359_at (11), 187436_s_at (9)
NM_066612 173806_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:9084789-9089275(-) 100.0 100.0
Public Domain and Genome References
Ensembl F02A9.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA79619 Hypothetical protein F02A9.4b [Caenorhabditis elegans] ref|NP_499012.2| F02A9.4b [Caenorhabditis elegans] sp|P34384|YLPD_CAEEL Hypothetical protein F02A9.4b in chromosome III 0.0
blast CAA79618 Hypothetical protein F02A9.4a [Caenorhabditis elegans] ref|NP_499013.1| F02A9.4a [Caenorhabditis elegans] sp|P34385|YLPC_CAEEL Hypothetical protein F02A9.4a in chromosome III 0.0
blast CAE62669 Hypothetical protein CBG06810 [Caenorhabditis briggsae] 0.0
blast NP_998092 methylcrotonoyl-Coenzyme A carboxylase 2 (beta) [Danio rerio] gb|AAH67577.1| Hypothetical protein zgc:85685 [Danio rerio] 0.0
blast XP_787663 PREDICTED: similar to mitochondrial ribosomal protein L44 [Strongylocentrotus purpuratus] 2.0E-35
Protein Families
Method ID Description E-Value
ec ACCD_ECOLI (P08193) Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (EC 6.4.1.2) (ACCase beta chain) 0.13
ec ACCD_ECOLI (P08193) Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (EC 6.4.1.2) (ACCase beta chain) 2.0E-73
Protein Domains
Database ID Description E-Value
Pfam IPR000022 EMBL-EBI Carboxyl transferase 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:187436_S_AT
gtaatgacatatacccacttgccgatcctcttgctgttgtaactgatttgttaaaatcta
acggtgtcactgaaattgagccacgagttcttcgatcggccggagagaacagcgcagagc
cgatctatgttgttgcaatttatgcggacaaattgaagaatgttggacaaagtgctggag
aatcattggcaattgctgttgatatggctgctagagaagcattgctcagactctgggata
ttacttcagaaaaagtcctcttctttggcgatcgtgctgccaatgtaccactggaacgat
actctcaaccgaatttctcattgtcacagaagtgctctccagggtatttgccaaaaccaa
tcttcaaaatcaatgaaaatctacttcttaatttcagaacaaacacgaacattattgacc
atagccaacctgttgaatcaccagacaatctaattgaagcagttctccgataccgaaatg
tggttgatgcagaagtaggaaagagctacactaaacgattgcgacacaaattctcgcgtg
gttcgcttgcgaaacggtccttcagatat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTAATGACATATACCCACTTGCCGA 687 461 656 Antisense
CGATCCTCTTGCTGTTGTAACTGAT 642 247 678 Antisense
TGAGCCACGAGTTCTTCGATCGGCC 86 571 732 Antisense
GATCGGCCGGAGAGAACAGCGCAGA 206 417 749 Antisense
ACAGCGCAGAGCCGATCTATGTTGT 273 109 764 Antisense
TCGTGCTGCCAATGTACCACTGGAA 392 603 927 Antisense
GATACTCTCAACCGAATTTCTCATT 669 423 953 Antisense
AGAAGTGCTCTCCAGGGTATTTGCC 487 75 983 Antisense
GAAGCAGTTCTCCGATACCGAAATG 690 339 1111 Antisense
ATTCTCGCGTGGTTCGCTTGCGAAA 251 11 1185 Antisense
GCTTGCGAAACGGTCCTTCAGATAT 122 307 1200 Antisense
Sequence Source Affymetrix Proprietary Database