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[187428_at]

Description T25E4.1 /REP_DB=WormBase Gene ID /WP=CE28493 /TR=SW:Q10014 /GB=AAC46730.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 53      6grp_high1_10_10 > 24      6grp_highest_nonorm > 20      6grp_total > 02      4grp_total > 49      6grp_highest_sub > 02      6grp_high1_sub > 02      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2319
1 2 3 4 range
A 81.7
(-3)
A 		2131.9
(3)
P 		2139.5
(3)
P 		158.7
(-2.8)
P 		2058
B 77
(-3)
A 		2367.8
(3)
P 		2385.1
(3)
P 		127.3
(-2.9)
A 		2308
C 168.2
(-3)
A 		2396.1
(3)
P 		2184.7
(2.4)
P 		151.1
(-3)
A 		2245
avr 109
(-3) 		2298.6
(3) 		2236.4
(2.8) 		145.7
(-2.9) 		2190

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1097
1 2 3 4 5 6 range
A 1309.5
(1.1)
P 		1626.9
(3)
P 		604.2
(-3)
P 		940.8
(-1)
P 		1203.7
(0.5)
P 		1113.4
(-0)
P 		1023
B 1115.8
(-0.5)
P 		1701.2
(3)
P 		826
(-2.3)
P 		706.5
(-3)
P 		1153.2
(-0.3)
P 		850.6
(-2.1)
P 		995
C 1137.7
(-0.6)
P 		1636.2
(3)
P 		797.5
(-3)
P 		1024.1
(-1.4)
P 		1135.7
(-0.6)
P 		803.4
(-3)
P 		839
avr 1187.7
(-0.1) 		1654.8
(3) 		742.6
(-3) 		890.5
(-2) 		1164.2
(-0.2) 		922.5
(-1.8) 		912
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 738
1 2 3 4 range
A 29.8
(-2.9)
A 		462.8
(1.9)
P 		559.3
(3)
P 		23.2
(-3)
P 		536
B 24.6
(-3)
A 		584.8
(3)
P 		552.7
(2.7)
P 		27.1
(-3)
A 		560
C 55.7
(-2.8)
A 		688.9
(2.4)
P 		761.4
(3)
P 		25.5
(-3)
A 		736
avr 36.7
(-2.9) 		578.8
(2.5) 		624.5
(3) 		25.3
(-3) 		599

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 447
1 2 3 4 5 6 range
A 241.4
(-1.5)
P 		578.6
(3)
P 		131.6
(-3)
P 		238.6
(-1.6)
P 		363.2
(0.1)
P 		271.1
(-1.1)
P 		447
B 388.2
(0.2)
P 		566.3
(3)
P 		200.6
(-2.8)
P 		187.1
(-3)
P 		468.5
(1.5)
P 		317.2
(-0.9)
P 		379
C 421.7
(1.4)
P 		499.2
(3)
P 		203
(-3)
P 		205.9
(-2.9)
P 		367.5
(0.3)
P 		268.9
(-1.7)
P 		296
avr 350.4
(-0.2) 		548
(3) 		178.4
(-3) 		210.5
(-2.5) 		399.7
(0.6) 		285.7
(-1.3) 		370
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187428_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4699
Sequence Type Exemplar sequence
Representative Public ID T25E4.1 NCBI
Target Description T25E4.1 /REP_DB=WormBase Gene ID /WP=CE28493 /TR=SW:Q10014 /GB=AAC46730.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062679(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062679 NCBI Caenorhabditis elegans T25E4.1 (T25E4.1) mRNA, complete cds. 11/11 None
SNAP00000027599 ENSEMBL cdna:SNAP chromosome:CEL140:II:5578371:5580338:1 11/11 None
GENEFINDER00000027601 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5578332:5579786:1 11/11 None
T25E4.1 ENSEMBL cdna:known chromosome:CEL140:II:5578290:5579935:1 gene:T25E4.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5578329-5579638(+) 99.59 99.59
Public Domain and Genome References
Gene Title
Ensembl T25E4.1
Entrez Gene 173943 Entrez gene
SwissProt Q10014 EMBL-EBI
WormBase CE28493 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC46730 Hypothetical protein T25E4.1 [Caenorhabditis elegans] ref|NP_495080.1| T25E4.1 [Caenorhabditis elegans] sp|Q10014|YR01_CAEEL Hypothetical protein T25E4.1 in chromosome II precursor 2.0E-68
blast T16942 hypothetical protein T25E4.1 - Caenorhabditis elegans 2.0E-68
blast CAE59141 Hypothetical protein CBG02445 [Caenorhabditis briggsae] 5.0E-66
blast CAE59141 Hypothetical protein CBG02445 [Caenorhabditis briggsae] 6.0E-66
Sequence
Target Sequence 
>C. ELEGANS:187428_AT
aacgtcgtcaatacattgctccactcgctggagcggctcaagttccacgtaaccctctgt
tcttcgcagctccagctcttccagtagctgctgctccagccctcgtccgaccggcattcg
ctccagtaccagttgccgcagctccagcttttgctccagttccagttgctgcaccaatgg
tccgcccaatgcttcaacagccagctattgttgctccagttgctccagttgttgcaccag
ttggacaatgcccaggaggaccatcccttccaattgaatgtgatccaaagagaccatggc
cacaatgcccaccacaatcttattgctatgccaccaactctgttgatattggaccatatt
tctgttgcccaatctggtccacctatggagcggcctggcgtccagctactccattctaca
actacgttccaccagttccagccaactggccagatgtagcaagaatgaccgctaattggc
ccgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACGTCGTCAATACATTGCTCCACT 122 145 134 Antisense
TCCACTCGCTGGAGCGGCTCAAGTT 417 589 153 Antisense
ACAGCCAGCTATTGTTGCTCCAGTT 474 109 330 Antisense
GTTGCACCAGTTGGACAATGCCCAG 484 439 364 Antisense
GACCATCCCTTCCAATTGAATGTGA 545 383 392 Antisense
AAGAGACCATGGCCACAATGCCCAC 234 155 421 Antisense
AATGCCCACCACAATCTTATTGCTA 218 167 437 Antisense
TGCTATGCCACCAACTCTGTTGATA 527 579 457 Antisense
ACCATATTTCTGTTGCCCAATCTGG 349 85 486 Antisense
CAATCTGGTCCACCTATGGAGCGGC 704 181 503 Antisense
CAAGAATGACCGCTAATTGGCCCGC 165 185 593 Antisense
Sequence Source Affymetrix Proprietary Database