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[187421_s_at]

Description B0244.7 /REP_DB=WormBase Gene ID /WP=CE26790 /TR=SW:Q09966 /GB=AAK68670.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 33      6grp_high1_10_10 > 22      4grp_high_8_8 > 11      6grp_highest_nonorm > 90      6grp_total > 22      4grp_total > 14      6grp_highest_sub > 02      6grp_high1_sub > De      6grp_total_sub > Hat      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 203
1 2 3 4 range
A 138.6
(3)
P 		109.3
(-2.2)
P 		104.8
(-3)
P 		138.8
(3)
P 		34
B 163.2
(3)
P 		110
(-2.3)
A 		103
(-3)
P 		147.8
(1.5)
P 		60
C 164.8
(0.2)
P 		55.6
(-3)
A 		67.4
(-2.7)
P 		258.5
(3)
P 		203
avr 155.5
(1.3) 		91.6
(-3) 		91.7
(-3) 		181.7
(3) 		90

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 74
1 2 3 4 5 6 range
A 124.3
(2.8)
P 		125
(2.9)
P 		125.7
(3)
P 		93.2
(-2.3)
P 		102.7
(-0.7)
P 		88.7
(-3)
P 		37
B 119.1
(0.8)
P 		136.7
(3)
P 		120.8
(1)
P 		131.4
(2.3)
P 		90.2
(-2.7)
P 		87.9
(-3)
P 		49
C 110.8
(0.5)
P 		142.1
(3)
P 		118.3
(1.1)
P 		100.9
(-0.3)
P 		68
(-3)
P 		85
(-1.6)
P 		74
avr 118.1
(0.9) 		134.6
(3) 		121.6
(1.4) 		108.5
(-0.3) 		87
(-3) 		87.2
(-3) 		48
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 39
1 2 3 4 range
A 50.5
(3)
P 		23.7
(-2.3)
P 		27.4
(-1.6)
P 		20.3
(-3)
P 		30
B 52.1
(3)
P 		27.2
(-2.3)
A 		23.9
(-3)
P 		31.5
(-1.4)
P 		28
C 54.6
(3)
P 		16
(-3)
A 		23.5
(-1.8)
P 		43.6
(1.3)
P 		39
avr 52.4
(3) 		22.3
(-3) 		24.9
(-2.5) 		31.8
(-1.1) 		30

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 25
1 2 3 4 5 6 range
A 22.9
(-2.7)
P 		44.5
(3)
P 		27.4
(-1.5)
P 		23.6
(-2.5)
P 		31
(-0.5)
P 		21.6
(-3)
P 		23
B 41.4
(1.5)
P 		45.5
(3)
P 		29.3
(-3)
P 		34.8
(-1)
P 		36.6
(-0.3)
P 		32.8
(-1.7)
P 		16
C 41.1
(2.4)
P 		43.4
(3)
P 		30.1
(-0.5)
P 		20.3
(-3)
P 		22
(-2.6)
P 		28.4
(-0.9)
P 		23
avr 35.1
(-0.1) 		44.5
(3) 		28.9
(-2.1) 		26.2
(-3) 		29.9
(-1.8) 		27.6
(-2.5) 		18
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 187421_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7464
Sequence Type Exemplar sequence
Representative Public ID B0244.7 NCBI
Target Description B0244.7 /REP_DB=WormBase Gene ID /WP=CE26790 /TR=SW:Q09966 /GB=AAK68670.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065839(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065839 NCBI Caenorhabditis elegans B0244.10 (B0244.10) mRNA, complete cds. 10/11 None
GENEFINDER00000016000 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:5731449:5737247:1 10/11 None
B0244.10 ENSEMBL cdna:known chromosome:CEL140:III:5732980:5737052:1 gene:B0244.10 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000015991 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:5731447-5737444(+) 98.03 100.0
Public Domain and Genome References
Gene Title
Ensembl B0244.10
Entrez Gene 175806 Entrez gene
SwissProt Q960A0 EMBL-EBI
WormBase CE26788 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK68668 Hypothetical protein B0244.10 [Caenorhabditis elegans] ref|NP_498240.1| B0244.10 [Caenorhabditis elegans] sp|Q960A0|YS9A_CAEEL Putative G-protein coupled receptor B0244.10 0.0
blast D88465 protein B0244.7 [imported] - Caenorhabditis elegans 0.0
blast AAK68670 Hypothetical protein B0244.7 [Caenorhabditis elegans] ref|NP_498239.1| B0244.7 [Caenorhabditis elegans] sp|Q09966|YS97_CAEEL Putative G-protein coupled receptor B0244.7 1.0E-180
blast D88465 protein B0244.7 [imported] - Caenorhabditis elegans 1.0E-164
blast CAE64330 Hypothetical protein CBG09010 [Caenorhabditis briggsae] 1.0E-147
Protein Domains
Database ID Description E-Value
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 2.2E-6
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_498240.1 13 51-73,85-107,127-149,161-183,228-250,263-282,318-349,362-384,394-427,440-462,487-509,536-558,578-597
B0244.10 13 51-73,85-107,127-149,161-183,228-250,263-282,318-349,362-384,394-427,440-462,487-509,536-558,578-597
GENEFINDER00000016000 17 51-73,94-116,131-153,166-188,208-230,261-283,303-325,364-386,401-423,465-487,507-538,551-573,583-616,629-651,676-698,725-747,767-786
Sequence
Target Sequence 
>C. ELEGANS:187421_S_AT
gatatacatcattgcattcgcgatagttttattttcggtttgtgttgcgattccatttgg
cttatttcaagcagccgacgaaatagacggccctataaaatgtgatagtgaaagtttttg
catagcatgtctcatccttatgggatgcaccggcacattgctctttgtcacagtatctct
ccactggcacagttataaatccaagaaaatgggaaacgtgtcatcttctgcattcaatca
tggaaaaagccgactaacatggacaactactattttggtcattttatgttgcgttgagtt
aataccaactgggttacttgcagcgtttggaaaatctgaatcaatatcagatgactgtta
cgatttctacaatgcaaatagtttaattttcccagctatcgtatcatcattggagacatt
tttaggatcaataacatttctactggacccaattataaacttttcctttgacaaacgcat
ttcgaaagttttcagttctcagtcaatatctcacctcacgagtaattttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATATACATCATTGCATTCGCGATA 300 427 1989 Antisense
ATAGCATGTCTCATCCTTATGGGAT 615 21 2110 Antisense
TTATGGGATGCACCGGCACATTGCT 647 679 2126 Antisense
ATTGCTCTTTGTCACAGTATCTCTC 210 7 2145 Antisense
GTATCTCTCCACTGGCACAGTTATA 288 455 2161 Antisense
AAACGTGTCATCTTCTGCATTCAAT 509 127 2202 Antisense
AGCCGACTAACATGGACAACTACTA 152 71 2236 Antisense
ACCAACTGGGTTACTTGCAGCGTTT 85 85 2292 Antisense
TTAATTTTCCCAGCTATCGTATCAT 349 685 2371 Antisense
ATAACATTTCTACTGGACCCAATTA 181 25 2419 Antisense
ATATCTCACCTCACGAGTAATTTTC 117 21 2494 Antisense
Sequence Source Affymetrix Proprietary Database