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[187419_at]

Description F54F2.7 /REP_DB=WormBase Gene ID /WP=CE00200 /TR=SW:P34452 /GB=AAK26132.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 94      4grp_high_8_8 > 14      6grp_total > 85      4grp_total > 09      6grp_high1_sub > In      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1174
1 2 3 4 range
A 181.4
(-2.9)
P 		163.4
(-3)
P 		222.3
(-2.5)
P 		921.2
(3)
P 		758
B 130.8
(-3)
A 		183.7
(-2.7)
P 		173.4
(-2.8)
P 		1304.5
(3)
P 		1174
C 144.4
(-3)
P 		207.8
(-2.6)
P 		262.8
(-2.2)
P 		1055.2
(3)
P 		911
avr 152.2
(-3) 		185
(-2.8) 		219.5
(-2.6) 		1093.6
(3) 		941

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 236
1 2 3 4 5 6 range
A 152.4
(-3)
A 		160
(-2.6)
P 		168.2
(-2.1)
P 		224.3
(1.1)
P 		256.9
(3)
M 		238.3
(1.9)
P 		105
B 118.5
(-2.4)
A 		177.7
(-0.9)
P 		95.1
(-3)
A 		331.5
(3)
P 		174.2
(-1)
P 		296.6
(2.1)
P 		236
C 162.9
(-3)
P 		164.6
(-2.9)
P 		173.5
(-2.5)
P 		277.5
(2.4)
P 		224.8
(-0.1)
P 		289.8
(3)
P 		127
avr 144.6
(-3) 		167.4
(-2) 		145.6
(-3) 		277.8
(3) 		218.6
(0.3) 		274.9
(2.9) 		133
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 243
1 2 3 4 range
A 66
(-1.2)
P 		35.5
(-3)
P 		58.1
(-1.6)
P 		134.8
(3)
P 		99
B 41.7
(-3)
A 		45.4
(-2.9)
P 		40.2
(-3)
P 		278
(3)
P 		238
C 47.8
(-3)
P 		59.7
(-2.5)
P 		91.6
(-1)
P 		177.9
(3)
P 		130
avr 51.8
(-2.8) 		46.9
(-3) 		63.3
(-2.3) 		196.9
(3) 		150

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 88
1 2 3 4 5 6 range
A 28.1
(-3)
A 		56.9
(0.5)
P 		36.6
(-2)
P 		56.9
(0.5)
P 		77.5
(3)
M 		58
(0.6)
P 		49
B 41.2
(-1.8)
A 		59.1
(-0.5)
P 		23.1
(-3)
A 		87.8
(1.4)
P 		70.8
(0.3)
P 		110.6
(3)
P 		88
C 60.4
(-1.2)
P 		50.2
(-2.3)
P 		44.1
(-3)
P 		55.8
(-1.7)
P 		72.7
(0.2)
P 		97
(3)
P 		53
avr 43.2
(-2) 		55.4
(-0.7) 		34.6
(-3) 		66.8
(0.6) 		73.7
(1.4) 		88.5
(3) 		54
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187419_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6905
Sequence Type Exemplar sequence
Representative Public ID F54F2.7 NCBI
Target Description F54F2.7 /REP_DB=WormBase Gene ID /WP=CE00200 /TR=SW:P34452 /GB=AAK26132.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066545(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066545 NCBI Caenorhabditis elegans F54F2.7 (F54F2.7) mRNA, complete cds. 9/11 None
SNAP00000044289 ENSEMBL cdna:SNAP chromosome:CEL140:III:8806266:8807248:-1 9/11 None
GENEFINDER00000044298 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:8806266:8807248:-1 11/11 None
F54F2.7 ENSEMBL cdna:known chromosome:CEL140:III:8806175:8807248:-1 gene:F54F2.7 9/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8806261-8807599(-) 86.01 100.0
Public Domain and Genome References
Gene Title
Ensembl F54F2.7
Entrez Gene 176238 Entrez gene
SwissProt P34452 EMBL-EBI
WormBase CE29395 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK26132 Hypothetical protein F54F2.7 [Caenorhabditis elegans] ref|NP_498946.1| F54F2.7 [Caenorhabditis elegans] sp|P34452|YMA7_CAEEL Hypothetical protein F54F2.7 in chromosome III 6.0E-86
blast AAK26132 Hypothetical protein F54F2.7 [Caenorhabditis elegans] ref|NP_498946.1| F54F2.7 [Caenorhabditis elegans] sp|P34452|YMA7_CAEEL Hypothetical protein F54F2.7 in chromosome III 2.0E-79
blast CAE62732 Hypothetical protein CBG06893 [Caenorhabditis briggsae] 1.0E-74
blast CAE62732 Hypothetical protein CBG06893 [Caenorhabditis briggsae] 1.0E-65
blast EAA08138 ENSANGP00000021928 [Anopheles gambiae str. PEST] ref|XP_312490.1| ENSANGP00000021928 [Anopheles gambiae str. PEST] 1.0E-11
Sequence
Target Sequence 
>C. ELEGANS:187419_AT
gtctggacactcatatgcttctgtagtcgatcgaatttattcaagaaaaagttcggaact
ttatgataatattgcatatcttcatcatccaagtggtgtcaccgttgtcgttcttcgaaa
tattcctgaaagtgaagttgtcgaagtggattttggaacgacgaagaagcacggcgcaga
tcgttcaacaaatcaagtttctggaaaaggaaaaaaaggagctcttattctacaacccga
ctcaaaattgtgtacattcaaatgcaaagatggttcagagcatgtcgtgagagcaggtgt
tcgtggaactcttgttgaggtacagtactctttaaaggcgcacgcgcgttttttatttaa
caaaaattgtcgtgtcgagaccgggtaccctaattttggcgacccgctcatgaacgatcg
cctgaaaactacaccagattttattagaacagcacctgataatcagggatttatcgcgat
tatcacctacggagccggagtacgtg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCTGGACACTCATATGCTTCTGTA 105 473 87 Antisense
CATCATCCAAGTGGTGTCACCGTTG 374 213 169 Antisense
GTCACCGTTGTCGTTCTTCGAAATA 623 465 184 Antisense
AGAAGCACGGCGCAGATCGTTCAAC 159 75 251 Antisense
AAAGGAGCTCTTATTCTACAACCCG 83 121 301 Antisense
GAGCATGTCGTGAGAGCAGGTGTTC 268 389 364 Antisense
GCGCACGCGCGTTTTTTATTTAACA 65 281 424 Antisense
TTTTGGCGACCCGCTCATGAACGAT 436 677 480 Antisense
GAACGATCGCCTGAAAACTACACCA 115 347 498 Antisense
GGATTTATCGCGATTATCACCTACG 616 509 553 Antisense
ATCACCTACGGAGCCGGAGTACGTG 633 17 568 Antisense
Sequence Source Affymetrix Proprietary Database