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[187417_s_at]

Description F02E8.5 /REP_DB=WormBase Gene ID /WP=CE07020 /TR=SW:Q19124 /GB=AAA96208.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 83      6grp_high1_10_10 > 48      6grp_highest_nonorm > 90      6grp_total > 48      4grp_total > 12      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 200
1 2 3 4 range
A 160.1
(-1.3)
P 		107.8
(-3)
M 		109.8
(-2.9)
P 		293.7
(3)
P 		186
B 194.4
(0.3)
P 		135.2
(-1.6)
A 		93.8
(-3)
A 		275.7
(3)
P 		182
C 123.8
(-2.2)
P 		146.4
(-1.5)
A 		95.8
(-3)
P 		294
(3)
P 		198
avr 159.4
(-1.1) 		129.8
(-2) 		99.8
(-3) 		287.8
(3) 		188

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 95
1 2 3 4 5 6 range
A 211.5
(3)
P 		124.2
(-3)
P 		148
(-1.4)
P 		159.5
(-0.6)
P 		144.4
(-1.6)
P 		166.3
(-0.1)
P 		87
B 170
(1.7)
P 		116.2
(-3)
P 		123.1
(-2.4)
P 		168.8
(1.6)
P 		184.3
(3)
P 		178
(2.4)
P 		68
C 127.1
(-2.9)
P 		192.4
(3)
P 		175.5
(1.5)
P 		129.9
(-2.7)
P 		126.1
(-3)
P 		152.8
(-0.6)
P 		66
avr 169.5
(3) 		144.3
(-3) 		148.9
(-1.9) 		152.7
(-1) 		151.6
(-1.3) 		165.7
(2.1) 		25
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 40
1 2 3 4 range
A 58.3
(3)
P 		23.4
(-3)
M 		28.7
(-2.1)
P 		43
(0.4)
P 		35
B 62
(3)
P 		33.4
(-1.3)
A 		21.7
(-3)
A 		58.8
(2.5)
P 		40
C 41
(-0.2)
P 		42.1
(0.2)
A 		33.4
(-3)
P 		49.6
(3)
P 		16
avr 53.8
(3) 		33
(-1.8) 		27.9
(-3) 		50.5
(2.2) 		26

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 49
1 2 3 4 5 6 range
A 39
(0.4)
P 		44.2
(3)
P 		32.2
(-3)
P 		40.4
(1.1)
P 		43.6
(2.7)
P 		40.5
(1.2)
P 		12
B 59.2
(0.9)
P 		38.7
(-1.8)
P 		29.9
(-3)
P 		44.7
(-1)
P 		74.9
(3)
P 		66.4
(1.9)
P 		45
C 47.1
(0.9)
P 		58.7
(3)
P 		44.7
(0.4)
P 		26.1
(-3)
P 		40.8
(-0.3)
P 		51.2
(1.6)
P 		33
avr 48.4
(1.4) 		47.2
(1) 		35.6
(-3) 		37.1
(-2.5) 		53.1
(3) 		52.7
(2.9) 		18
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 187417_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16072
Sequence Type Exemplar sequence
Representative Public ID F02E8.5 NCBI
Target Description F02E8.5 /REP_DB=WormBase Gene ID /WP=CE07020 /TR=SW:Q19124 /GB=AAA96208.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076367(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076367 NCBI Caenorhabditis elegans F02E8.5 (F02E8.5) mRNA, complete cds. 11/11 None
GENEFINDER00000018052 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:4459730:4462582:-1 11/11 None
SNAP00000018048 ENSEMBL cdna:SNAP chromosome:CEL140:X:4459842:4462429:-1 9/11 None
F02E8.5 ENSEMBL cdna:known chromosome:CEL140:X:4459622:4462582:-1 gene:F02E8.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:4459842-4462583(-) 97.99 100.0
Public Domain and Genome References
Gene Title
Ensembl F02E8.5
Entrez Gene 180717 Entrez gene
SwissProt Q19124 EMBL-EBI
WormBase CE07020 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:248543_AT transducin family protein / WD-40 repeat family protein at
CANINE_2:CFAAFFX.18299.1.S1_S_AT similar to Autophagy protein 16-like (APG16-like) cfa
CANINE_2:CFAAFFX.18329.1.S1_S_AT similar to Autophagy protein 16-like (APG16-like) cfa
DROSGENOME1:151111_AT dm
DROSOPHILA_2:1633643_A_AT dm
DROSGENOME1:150849_AT dm
DROSGENOME1:141401_AT dm
CHICKEN:GGAAFFX.1064.1.S1_S_AT ATG16 autophagy related 16-like 1 (S. cerevisiae) gga
CHICKEN:GGAAFFX.21700.1.S1_S_AT ATG16 autophagy related 16-like 1 (S. cerevisiae) gga
CHICKEN:GGAAFFX.24656.1.S1_S_AT ATG16 autophagy related 16-like 1 (S. cerevisiae) gga
HG-U133_PLUS_2:220521_S_AT ATG16 autophagy related 16-like 1 (S. cerevisiae) hs
HG-U133A:220521_S_AT ATG16 autophagy related 16-like 1 (S. cerevisiae) hs
HG-U133A_2:220521_S_AT ATG16 autophagy related 16-like 1 (S. cerevisiae) hs
HU35KSUBA:RC_AA112287_AT ATG16 autophagy related 16-like 1 (S. cerevisiae) hs
U133_X3P:G8922193_3P_S_AT ATG16 autophagy related 16-like 1 (S. cerevisiae) hs
U133_X3P:HS.16390.2.S1_3P_S_AT ATG16 autophagy related 16-like 1 (S. cerevisiae) hs
HG-U133_PLUS_2:232612_S_AT ATG16 autophagy related 16-like 1 (S. cerevisiae) hs
HG-U133B:232612_S_AT ATG16 autophagy related 16-like 1 (S. cerevisiae) hs
HG-U95B:44719_AT ATG16 autophagy related 16-like 1 (S. cerevisiae) hs
HG-U95E:85197_AT ATG16 autophagy related 16-like 1 (S. cerevisiae) hs
HG-U95D:85174_R_AT ATG16 autophagy related 16-like 1 (S. cerevisiae) hs
MOUSE430_2:1441852_X_AT autophagy-related 16-like 1 (yeast) mm
MOE430B:1441852_X_AT autophagy-related 16-like 1 (yeast) mm
MG-U74AV2:160581_AT autophagy-related 16-like 1 (yeast) mm
MOE430A:1428169_AT autophagy-related 16-like 1 (yeast) mm
MOUSE430A_2:1428169_AT autophagy-related 16-like 1 (yeast) mm
MOUSE430_2:1428169_AT autophagy-related 16-like 1 (yeast) mm
MU19KSUBA:TC21068_AT Autophagy-related 16-like (yeast) (Atg16l), transcript variant b, mRNA mm
MU19KSUBA:TC21068_G_AT Autophagy-related 16-like (yeast) (Atg16l), transcript variant b, mRNA mm
MU19KSUBC:TC38846_AT Autophagy-related 16-like (yeast) (Atg16l), transcript variant b, mRNA mm
RAE230A:1372375_AT APG16 autophagy 16-like (S. cerevisiae) (predicted) rn
RAT230_2:1372375_AT APG16 autophagy 16-like (S. cerevisiae) (predicted) rn
RG-U34A:RC_AA892083_AT APG16 autophagy 16-like (S. cerevisiae) (predicted) rn
Protein Similarities
Method ID Description E-Value
blast AAA96208 Hypothetical protein F02E8.5 [Caenorhabditis elegans] ref|NP_508768.1| F02E8.5 [Caenorhabditis elegans] sp|Q19124|YDEM_CAEEL Hypothetical WD-repeat protein F02E8.5 in chromosome X 0.0
blast CAE68597 Hypothetical protein CBG14468 [Caenorhabditis briggsae] 0.0
blast CAE59321 Hypothetical protein CBG02663 [Caenorhabditis briggsae] 1.0E-114
Protein Domains
Database ID Description E-Value
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 3.2E-6
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 1.8E-4
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 2.4E-10
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.18
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.38
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.068
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.0026
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.0020
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 3.2E-6
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 1.8E-4
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 2.4E-10
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.18
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.38
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.021
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_508768.1 1 21-43
F02E8.5 1 21-43
GENEFINDER00000018052 1 21-43
Sequence
Target Sequence 
>C. ELEGANS:187417_S_AT
tttgagtactctctctgggcattctgatcaagtaacatgcgtcaagttttatcaatcaca
ttcggcggtttctggcagcgcagaccgggttatcaaaatttgggatattcaaaatcaaag
atgcagccgctctctattccctgcgtctaaggttctcgacgttgcaaccaatatgggtgc
aagcccatcactattcgcatcaggacattttgacaaaaaactaagattttacgatggaag
gtcaacggatccagttagaactgttgacatgggaggtagaattacttctttggatgttac
aatgagtggatgtgaacttctcgtttcgactcgtgatgacactatctccctgattgatct
acgaacgttccagacggttcactgttacagcgcggaaaactaccgtactagttccgacct
ttcaagagttgttttatcatccggaaatgaatacgtcgctgcaggaagttcgaatggaag
catcttcgtctggaatagaaatagtactaagctagaaaaacagctttgcagtaactcgga
aaatgccatattctcactttcatggaacccaac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTTGAGTACTCTCTCTGGGCATTCT 32 667 1122 Antisense
CAATCACATTCGGCGGTTTCTGGCA 337 181 1174 Antisense
TGGCAGCGCAGACCGGGTTATCAAA 406 545 1194 Antisense
TTCCCTGCGTCTAAGGTTCTCGACG 485 697 1258 Antisense
CCCATCACTATTCGCATCAGGACAT 430 259 1305 Antisense
GGATGTGAACTTCTCGTTTCGACTC 435 509 1429 Antisense
TCGACTCGTGATGACACTATCTCCC 95 603 1447 Antisense
CACTATCTCCCTGATTGATCTACGA 286 197 1461 Antisense
GTTCCAGACGGTTCACTGTTACAGC 226 439 1488 Antisense
GTACTAGTTCCGACCTTTCAAGAGT 435 457 1526 Antisense
TATTCTCACTTTCATGGAACCCAAC 365 655 1670 Antisense
Sequence Source Affymetrix Proprietary Database