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[187401_at]

Description K10D2.5 /REP_DB=WormBase Gene ID /WP=CE02017 /TR=SW:Q09411 /GB=AAA62542.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 59      6grp_high1_10_10 > 94      4grp_highest_8_8 > 15      4grp_high_8_8 > 06      6grp_highest_nonorm > 92      6grp_total > 85      4grp_total > 02      6grp_highest_sub > In      6grp_high1_sub > In      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 393
1 2 3 4 range
A 143.3
(-3)
P 		171.6
(-2.5)
P 		193.9
(-2.1)
P 		473.4
(3)
P 		330
B 137.7
(-3)
P 		181.3
(-2)
P 		175.3
(-2.1)
P 		399.3
(3)
P 		262
C 197.7
(-2.4)
P 		168
(-2.9)
P 		163.8
(-3)
P 		530.2
(3)
P 		366
avr 159.6
(-3) 		173.6
(-2.7) 		177.7
(-2.6) 		467.6
(3) 		308

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 490
1 2 3 4 5 6 range
A 106.9
(-3)
P 		138.1
(-2.5)
P 		400.4
(1.3)
P 		519
(3)
P 		372.7
(0.9)
P 		449.9
(2)
P 		412
B 146.8
(-3)
P 		221
(-2)
P 		393.9
(0.3)
P 		352.3
(-0.3)
P 		597.2
(3)
P 		513.7
(1.9)
P 		450
C 140.6
(-3)
P 		262.7
(-1.3)
P 		255.9
(-1.4)
P 		375.9
(0.3)
P 		395.4
(0.6)
P 		563.9
(3)
P 		423
avr 131.4
(-3) 		207.3
(-1.8) 		350.1
(0.5) 		415.7
(1.5) 		455.1
(2.1) 		509.2
(3) 		378
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 52
1 2 3 4 range
A 52.2
(-0.2)
P 		37.2
(-3)
P 		50.7
(-0.5)
P 		69.3
(3)
P 		32
B 43.9
(-2.6)
P 		44.8
(-2.4)
P 		40.6
(-3)
P 		85.1
(3)
P 		45
C 65.4
(-0.5)
P 		48.3
(-3)
P 		57.1
(-1.7)
P 		89.4
(3)
P 		41
avr 53.8
(-1.4) 		43.4
(-3) 		49.5
(-2) 		81.3
(3) 		38

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 223
1 2 3 4 5 6 range
A 19.7
(-3)
P 		49.1
(-1.4)
P 		87.2
(0.6)
P 		131.6
(3)
P 		112.5
(2)
P 		109.5
(1.8)
P 		112
B 51.1
(-3)
P 		73.5
(-2.3)
P 		95.6
(-1.6)
P 		93.3
(-1.7)
P 		242.6
(3)
P 		191.6
(1.4)
P 		192
C 52.1
(-3)
P 		80.2
(-1.8)
P 		65.1
(-2.4)
P 		75.6
(-2)
P 		128
(0.3)
P 		188.8
(3)
P 		137
avr 41
(-3) 		67.6
(-1.7) 		82.6
(-1) 		100.2
(-0.1) 		161
(2.9) 		163.3
(3) 		122
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187401_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6158
Sequence Type Exemplar sequence
Representative Public ID K10D2.5 NCBI
Target Description K10D2.5 /REP_DB=WormBase Gene ID /WP=CE02017 /TR=SW:Q09411 /GB=AAA62542.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065696(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065696 NCBI Caenorhabditis elegans K10D2.5 (K10D2.5) mRNA, complete cds. 11/11 None
SNAP00000029355 ENSEMBL cdna:SNAP chromosome:CEL140:III:5172747:5173193:1 11/11 None
GENEFINDER00000029362 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:5172747:5173193:1 11/11 None
K10D2.5 ENSEMBL cdna:known chromosome:CEL140:III:5172742:5173222:1 gene:K10D2.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:5172745-5173192(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl K10D2.5
Entrez Gene 175706 Entrez gene
SwissProt Q09411 EMBL-EBI
WormBase CE02017 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA62542 Hypothetical protein K10D2.5 [Caenorhabditis elegans] ref|NP_498097.1| K10D2.5 [Caenorhabditis elegans] sp|Q09411|YRJ5_CAEEL Hypothetical protein K10D2.5 in chromosome III 4.0E-56
blast CAE73780 Hypothetical protein CBG21325 [Caenorhabditis briggsae] 3.0E-31
Protein Domains
Database ID Description E-Value
Pfam IPR001199 EMBL-EBI Cytochrome b5 1.7E-4
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_498097.1 1 10-27
K10D2.5 1 10-27
SNAP00000029355 1 10-27
GENEFINDER00000029362 1 10-27
Sequence
Target Sequence 
>C. ELEGANS:187401_AT
aaatcggggaatatgagctcactttcatcgatgttgcggtgtttgtggtgtttctcgtgg
ctttgaataaaattgttaaaaggatgcttgttgcaaaaatcaatgagccagcgaaatatg
agatcgaacagctcgaagagcgtgatatgacaatggaagaaatcgaaagcatgcgccgcg
aggagaacaggtgcctggtcattgtggaagacaaaatttacgacctctctggctcacaag
atctctatgacaacaaccgcgatcttttcgagagtagtgaaggatgtggacctgaatggg
caccgat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAATCGGGGAATATGAGCTCACTTT 115 115 17 Antisense
AGCTCACTTTCATCGATGTTGCGGT 654 77 32 Antisense
GATGTTGCGGTGTTTGTGGTGTTTC 448 409 46 Antisense
TGGTGTTTCTCGTGGCTTTGAATAA 269 553 62 Antisense
AAATCGAAAGCATGCGCCGCGAGGA 109 119 176 Antisense
TGCGCCGCGAGGAGAACAGGTGCCT 270 583 188 Antisense
ACAGGTGCCTGGTCATTGTGGAAGA 383 99 203 Antisense
GACAAAATTTACGACCTCTCTGGCT 175 365 226 Antisense
CTCTCTGGCTCACAAGATCTCTATG 554 229 241 Antisense
CTATGACAACAACCGCGATCTTTTC 193 225 261 Antisense
GATGTGGACCTGAATGGGCACCGAT 43 409 299 Antisense
Sequence Source Affymetrix Proprietary Database