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[187391_at]

Description F40B5.1 /REP_DB=WormBase Gene ID /WP=CE01935 /TR=SW:Q09319 /GB=AAA64336.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 91      4grp_total > 03      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 127
1 2 3 4 range
A 23.2
(-3)
A 		31.4
(-2.6)
A 		30.2
(-2.7)
A 		146.7
(3)
P 		124
B 42.7
(0.5)
P 		27.1
(-1.9)
A 		19.4
(-3)
A 		59.6
(3)
P 		40
C 37.5
(-2.3)
P 		26.7
(-3)
A 		27.6
(-2.9)
A 		114.2
(3)
P 		88
avr 34.5
(-2.3) 		28.4
(-2.8) 		25.7
(-3) 		106.8
(3) 		81

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 53
1 2 3 4 5 6 range
A 31.9
(0.5)
A 		2
(-3)
A 		27.3
(-0.1)
P 		18.1
(-1.1)
A 		53.5
(3)
P 		2.7
(-2.9)
A 		52
B 18.1
(0.6)
A 		21.3
(1.3)
A 		2.6
(-2.7)
A 		29.3
(3)
A 		23.4
(1.7)
A 		1
(-3)
A 		28
C 4.2
(-2.8)
A 		52
(3)
P 		2.7
(-3)
A 		39.8
(1.5)
A 		16.6
(-1.3)
A 		4.7
(-2.8)
A 		49
avr 18.1
(0.2) 		25.1
(1.7) 		10.9
(-1.3) 		29.1
(2.6) 		31.2
(3) 		2.8
(-3) 		28
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 17
1 2 3 4 range
A 8.4
(-2.3)
A 		6.8
(-3)
A 		7.9
(-2.6)
A 		21.5
(3)
P 		15
B 13.6
(3)
P 		6.7
(-1.5)
A 		4.5
(-3)
A 		12.7
(2.4)
P 		9
C 12.4
(-0.6)
P 		7.7
(-3)
A 		9.6
(-2)
A 		19.3
(3)
P 		12
avr 11.5
(-0.5) 		7.1
(-3) 		7.3
(-2.9) 		17.8
(3) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 16
1 2 3 4 5 6 range
A 5.9
(-1)
A 		0.7
(-3)
A 		5.9
(-1)
P 		4.6
(-1.5)
A 		16.2
(3)
P 		0.7
(-3)
A 		16
B 6.3
(0.9)
A 		7.1
(1.4)
A 		0.6
(-2.9)
A 		7.8
(1.9)
A 		9.5
(3)
A 		0.4
(-3)
A 		9
C 1.5
(-2.7)
A 		15.9
(3)
P 		0.7
(-3)
A 		8
(-0.1)
A 		5.4
(-1.1)
A 		1.6
(-2.6)
A 		15
avr 4.6
(-0.7) 		7.9
(1.4) 		2.4
(-2.1) 		6.8
(0.7) 		10.4
(3) 		0.9
(-3) 		10
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187391_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16468
Sequence Type Exemplar sequence
Representative Public ID F40B5.1 NCBI
Target Description F40B5.1 /REP_DB=WormBase Gene ID /WP=CE01935 /TR=SW:Q09319 /GB=AAA64336.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077019(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077019 NCBI Caenorhabditis elegans F40B5.1 (F40B5.1) mRNA, complete cds. 10/11 None
GENEFINDER00000013914 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:8375225:8377241:1 10/11 None
F40B5.1 ENSEMBL cdna:known chromosome:CEL140:X:8376561:8377241:1 gene:F40B5.1 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000013912 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:8375223-8377240(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F40B5.1
Entrez Gene 185512 Entrez gene
SwissProt Q09319 EMBL-EBI
WormBase CE35529 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4245 neprilysin activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA64336 Hypothetical protein F40B5.1 [Caenorhabditis elegans] ref|NP_509420.2| F40B5.1 [Caenorhabditis elegans] sp|Q09319|YQZ1_CAEEL Hypothetical protein F40B5.1 in chromosome X 1.0E-100
blast AAA64336 Hypothetical protein F40B5.1 [Caenorhabditis elegans] ref|NP_509420.2| F40B5.1 [Caenorhabditis elegans] sp|Q09319|YQZ1_CAEEL Hypothetical protein F40B5.1 in chromosome X 9.0E-79
blast AAC48280 Hypothetical protein C53B7.7 [Caenorhabditis elegans] ref|NP_509156.2| C53B7.7 [Caenorhabditis elegans] 8.0E-43
blast AAC48280 Hypothetical protein C53B7.7 [Caenorhabditis elegans] ref|NP_509156.2| C53B7.7 [Caenorhabditis elegans] 4.0E-39
blast CAE65809 Hypothetical protein CBG10917 [Caenorhabditis briggsae] 6.0E-32
blast CAE65805 Hypothetical protein CBG10913 [Caenorhabditis briggsae] 1.0E-27
Protein Domains
Database ID Description E-Value
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 1.6E-28
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 1.4E-28
Sequence
Target Sequence 
>C. ELEGANS:187391_AT
gcaaagctacctatggtgcatttgaaaatggagattttttctgttttgttttgtcttcaa
gtaaatttcgagagccggataaaacgattgatcggctcggcatcacatatcagaaactgt
ttttctattcatacgcatttaatttttgcagaggtgaacagtcgacatatcaaccagatc
attcagctcacaacatcagaatcaatgttgtagctcaaaatcctggtttccaagaagctt
tccagtgttctgaagattcaccaatgatgcagtccgcaactgagcagtgtttcatctacg
gcaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCAAAGCTACCTATGGTGCATTTGA 72 319 647 Antisense
AAAACGATTGATCGGCTCGGCATCA 562 131 727 Antisense
GGCTCGGCATCACATATCAGAAACT 501 539 740 Antisense
AACTGTTTTTCTATTCATACGCATT 403 143 761 Antisense
GAACAGTCGACATATCAACCAGATC 277 349 802 Antisense
CAACCAGATCATTCAGCTCACAACA 120 187 817 Antisense
GTAGCTCAAAATCCTGGTTTCCAAG 198 457 856 Antisense
GAAGCTTTCCAGTGTTCTGAAGATT 279 343 880 Antisense
GATTCACCAATGATGCAGTCCGCAA 417 431 901 Antisense
GATGCAGTCCGCAACTGAGCAGTGT 512 411 912 Antisense
TGAGCAGTGTTTCATCTACGGCAGA 505 569 927 Antisense
Sequence Source Affymetrix Proprietary Database