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[187365_at]

Description B0280.2 /REP_DB=WormBase Gene ID /WP=CE00734 /TR=SW:P41993 /GB=AAA19081.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 13      4grp_highest_8_8 > 43      4grp_high_8_8 > 48      6grp_total > 15      4grp_total > 55      6grp_high1_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 181
1 2 3 4 range
A 324.5
(3)
P 		215.8
(-3)
P 		253.7
(-0.9)
P 		224.3
(-2.5)
P 		109
B 306.2
(3)
P 		172.7
(-1.9)
P 		165.1
(-2.2)
P 		143.4
(-3)
P 		163
C 296.4
(3)
P 		172.9
(-3)
P 		277.3
(2.1)
P 		226.3
(-0.4)
P 		124
avr 309
(3) 		187.1
(-3) 		232
(-0.8) 		198
(-2.5) 		122

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 181
1 2 3 4 5 6 range
A 162.2
(0.6)
P 		211.2
(3)
P 		112.1
(-2)
P 		141.8
(-0.5)
P 		167.1
(0.8)
M 		91.1
(-3)
P 		120
B 189.6
(3)
P 		176.7
(2.3)
P 		131.5
(-0)
P 		135.3
(0.2)
P 		84.9
(-2.5)
P 		75
(-3)
P 		115
C 247.3
(2.8)
P 		252.7
(3)
P 		181.7
(0.6)
P 		72
(-3)
A 		121.2
(-1.4)
P 		93.7
(-2.3)
P 		181
avr 199.7
(2.3) 		213.5
(3) 		141.8
(-0.4) 		116.4
(-1.6) 		124.4
(-1.2) 		86.6
(-3) 		127
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 88
1 2 3 4 range
A 118.2
(3)
P 		46.8
(-2)
P 		66.3
(-0.6)
P 		32.8
(-3)
P 		85
B 97.6
(3)
P 		42.6
(-1.9)
P 		38.2
(-2.3)
P 		30.6
(-3)
P 		67
C 98.1
(3)
P 		49.7
(-1.8)
P 		96.6
(2.8)
P 		38.2
(-3)
P 		60
avr 104.6
(3) 		46.4
(-1.9) 		67
(-0.2) 		33.9
(-3) 		71

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 77
1 2 3 4 5 6 range
A 29.9
(-2.1)
P 		75.1
(3)
P 		24.4
(-2.8)
P 		36
(-1.4)
P 		50.4
(0.2)
M 		22.2
(-3)
P 		53
B 66
(3)
P 		58.8
(1.9)
P 		31.9
(-2.4)
P 		35.8
(-1.8)
P 		34.5
(-2)
P 		28
(-3)
P 		38
C 91.7
(3)
P 		77.1
(1.9)
P 		46.2
(-0.5)
P 		14.5
(-3)
A 		39.2
(-1.1)
P 		31.4
(-1.7)
P 		77
avr 62.5
(1.9) 		70.3
(3) 		34.2
(-2) 		28.8
(-2.8) 		41.4
(-1) 		27.2
(-3) 		43
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187365_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7157
Sequence Type Exemplar sequence
Representative Public ID B0280.2 NCBI
Target Description B0280.2 /REP_DB=WormBase Gene ID /WP=CE00734 /TR=SW:P41993 /GB=AAA19081.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171181(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171181 NCBI Caenorhabditis elegans B0280.13 (B0280.13) mRNA, complete cds. 10/11 None
SNAP00000041613 ENSEMBL cdna:SNAP chromosome:CEL140:III:7128832:7131923:1 10/11 None
GENEFINDER00000041621 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:7128832:7131915:1 10/11 None
B0280.13 ENSEMBL cdna:known chromosome:CEL140:III:7128825:7131972:1 gene:B0280.13 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_182249 2/11 Cross Hyb Matching Probes None
SNAP00000002048 2/11 Cross Hyb Matching Probes None
GENEFINDER00000002056 2/11 Cross Hyb Matching Probes None
C31C9.6 2/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:7124608-7131921(+) 98.89 100.0
chrII:13655906-13661509(-) 50.34 50.34
Public Domain and Genome References
Gene Title
Ensembl B0280.13
Entrez Gene 3565684 Entrez gene
SwissProt Q5WRU4 EMBL-EBI
WormBase CE37472 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAU87834 Hypothetical protein B0280.13 [Caenorhabditis elegans] ref|NP_741212.2| B0280.13 [Caenorhabditis elegans] 0.0
blast CAD57687 Hypothetical protein C31C9.6 [Caenorhabditis elegans] ref|NP_872049.1| C31C9.6 [Caenorhabditis elegans] 0.0
blast T15297 hypothetical protein B0280.2 - Caenorhabditis elegans 0.0
Sequence
Target Sequence 
>C. ELEGANS:187365_AT
atcccgattggacatctaattccacgagttccatgtctgcgtcgttccggtcttcgtcta
cagtaaccgcagcaacatcattcaaacatagtcacacttttgctgcggatcccatcaaga
ctacggactgggaaactccgagaaaagatacttatggatctacaaatcagcgacagcctc
aaaacatttataaattcgaacaagaacacgttgaacaaacttatcaaattgagaaaattg
aaatggacgatacaccgccagagttgccaagtagaggtgcttcaagaaatccagaagatc
agatgactagaggaggtctctcaactcctcgaattccaactcatcaatctaattctgatc
gaagaagtctaatggatcaaatccgttcaaccgatcgttcacaagtcttgcgtaaagtca
gtgatacacctgaaacgaggcaatctgtcccatcaacgacgtctggaac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCCCGATTGGACATCTAATTCCAC 131 39 2498 Antisense
CTAATTCCACGAGTTCCATGTCTGC 165 227 2513 Antisense
TCGTCTACAGTAACCGCAGCAACAT 374 605 2551 Antisense
GCGGATCCCATCAAGACTACGGACT 654 291 2602 Antisense
GATCTACAAATCAGCGACAGCCTCA 110 419 2654 Antisense
TACACCGCCAGAGTTGCCAAGTAGA 515 637 2748 Antisense
GAGGAGGTCTCTCAACTCCTCGAAT 80 403 2807 Antisense
ATTCCAACTCATCAATCTAATTCTG 550 7 2830 Antisense
CAAATCCGTTCAACCGATCGTTCAC 564 191 2875 Antisense
CTGAAACGAGGCAATCTGTCCCATC 177 237 2927 Antisense
CTGTCCCATCAACGACGTCTGGAAC 696 237 2942 Antisense
Sequence Source Affymetrix Proprietary Database