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[187360_at]

Description F32A6.3 /REP_DB=WormBase Gene ID /WP=CE04471 /TR=SW:Q19954 /GB=AAA81388.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 14      6grp_high1_10_10 > 20      4grp_highest_8_8 > 41      4grp_high_8_8 > 49      6grp_highest_nonorm > 81      6grp_total > 41      4grp_total > 53      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 213
1 2 3 4 range
A 323.1
(3)
P 		252.1
(1)
P 		259.3
(1.2)
P 		115
(-3)
P 		208
B 316.5
(3)
P 		226.7
(-1.5)
P 		218.5
(-1.9)
P 		197.1
(-3)
P 		119
C 327.5
(3)
P 		284.3
(0.9)
P 		246.7
(-0.9)
P 		201.8
(-3)
P 		126
avr 322.4
(3) 		254.4
(0.3) 		241.5
(-0.2) 		171.3
(-3) 		151

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 182
1 2 3 4 5 6 range
A 345.4
(3)
P 		285
(0.1)
P 		310.9
(1.3)
P 		240.1
(-2.1)
P 		222.2
(-3)
P 		240
(-2.1)
P 		123
B 256.2
(-0.8)
P 		251.6
(-0.9)
P 		371.1
(3)
P 		277.2
(-0.1)
P 		189
(-3)
P 		190.8
(-2.9)
P 		182
C 252
(-1.3)
P 		315.7
(1.1)
P 		305
(0.7)
P 		368.6
(3)
P 		233.9
(-2)
P 		205.7
(-3)
P 		163
avr 284.5
(0.7) 		284.1
(0.7) 		329
(3) 		295.3
(1.3) 		215
(-2.9) 		212.2
(-3) 		117
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 101
1 2 3 4 range
A 117.7
(3)
P 		54.7
(-0.7)
P 		67.8
(0)
P 		16.8
(-3)
P 		101
B 100.9
(3)
P 		56
(-1.6)
P 		50.6
(-2.1)
P 		42
(-3)
P 		59
C 108.4
(3)
P 		81.7
(0.8)
P 		86
(1.2)
P 		34
(-3)
P 		74
avr 109
(3) 		64.1
(-0.4) 		68.1
(-0.1) 		30.9
(-3) 		78

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 43
1 2 3 4 5 6 range
A 63.7
(-2.3)
P 		101.4
(3)
P 		67.7
(-1.7)
P 		60.9
(-2.7)
P 		67
(-1.8)
P 		58.4
(-3)
P 		43
B 89.1
(2.7)
P 		83.7
(1)
P 		90.1
(3)
P 		73.4
(-2.3)
P 		76.8
(-1.2)
P 		71.1
(-3)
P 		19
C 93.4
(2.4)
P 		96.3
(3)
P 		77.6
(-1.1)
P 		74.1
(-1.8)
P 		75.7
(-1.5)
P 		68.8
(-3)
P 		28
avr 82.1
(0.5) 		93.8
(3) 		78.5
(-0.3) 		69.5
(-2.3) 		73.2
(-1.5) 		66.1
(-3) 		28
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187360_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17260
Sequence Type Exemplar sequence
Representative Public ID F32A6.3 NCBI
Target Description F32A6.3 /REP_DB=WormBase Gene ID /WP=CE04471 /TR=SW:Q19954 /GB=AAA81388.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076497(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076497 NCBI Caenorhabditis elegans related to yeast Vacuolar Protein Sorting factor family member (vps-41) (vps-41) mRNA, complete cds. 11/11 None
GENEFINDER00000030571 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:5285689:5289505:1 11/11 None
SNAP00000030562 ENSEMBL cdna:SNAP chromosome:CEL140:X:5285911:5289505:1 11/11 None
F32A6.3 ENSEMBL cdna:known chromosome:CEL140:X:5283796:5289659:1 gene:F32A6.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:5285450-5289506(+) 91.7 100.0
Public Domain and Genome References
Ensembl F32A6.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA81388 Related to yeast vacuolar protein sorting factor protein 41, isoform a [Caenorhabditis elegans] ref|NP_508898.2| related to yeast Vacuolar Protein Sorting factor family member (vps-41) [Caenorhabditis elegans] sp|Q19954|VPS41_CAEEL Vacuolar assembly protein VPS41 homolog 0.0
blast T16236 hypothetical protein F32A6.3 - Caenorhabditis elegans 0.0
blast AAZ91350 Related to yeast vacuolar protein sorting factor protein 41, isoform b [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000547 EMBL-EBI 7-Fold repeat in clathrin and VPS proteins 4.6E-6
Pfam IPR000547 EMBL-EBI 7-Fold repeat in clathrin and VPS proteins 4.6E-6
Pfam IPR000547 EMBL-EBI 7-Fold repeat in clathrin and VPS proteins 4.6E-6
Sequence
Target Sequence 
>C. ELEGANS:187360_AT
gagacaatctatcttcttgccaaaagtggaaatcattatgatgctgtgaaaatgatggtt
cgagagtatcggaacatggaaaaagtcatcgattactgcaaagatcaaaatgaccccgat
ttatggattcaccttctaggagtagttgccgagtttcctgctcatttttcgcagcttatc
attgaagcgtcaaactgcctcgatcctctcctgattatggacaaactaccggacgattcg
gatattcctaacttgagtgaagcgctcgacaaacttctcgttgactatacaaatcatgca
gagcttcagcaatgttgctacgactcaaccttgaacgatctgaatgttctcactcaagga
ctgatatcggcagctgatgaatcagtttccgtgaacatagtatccagatgttcattatgt
gctcaaatcatcatcaattctaaccaagaaacaacaaaaaagttttcggatataaaagtg
ttcaaatgcggacacatttttcacttggcttgttctacttctgagatggaacgccgtcaa
tcaattgaggagggcctctgcattgcgtg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGACAATCTATCTTCTTGCCAAAA 413 393 1906 Antisense
GACCCCGATTTATGGATTCACCTTC 561 369 2017 Antisense
AGGAGTAGTTGCCGAGTTTCCTGCT 699 55 2043 Antisense
TTTCCTGCTCATTTTTCGCAGCTTA 384 669 2059 Antisense
ATTGAAGCGTCAAACTGCCTCGATC 340 5 2086 Antisense
CTCGATCCTCTCCTGATTATGGACA 80 233 2104 Antisense
AGCGCTCGACAAACTTCTCGTTGAC 486 81 2166 Antisense
GCAATGTTGCTACGACTCAACCTTG 365 321 2214 Antisense
ATGCGGACACATTTTTCACTTGGCT 606 41 2391 Antisense
TCACTTGGCTTGTTCTACTTCTGAG 58 627 2406 Antisense
TTGAGGAGGGCCTCTGCATTGCGTG 706 679 2450 Antisense
Sequence Source Affymetrix Proprietary Database