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[187357_at]

Description C09F5.2 /REP_DB=WormBase Gene ID /WP=CE01774 /TR=SW:Q09232 /GB=AAA64507.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 76      6grp_high1_10_10 > 66      4grp_highest_8_8 > 42      4grp_high_8_8 > 49      6grp_highest_nonorm > 85      6grp_total > 67      4grp_total > 62      6grp_highest_sub > Da      6grp_high1_sub > Da      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 325
1 2 3 4 range
A 631.9
(3)
P 		492.2
(-0.2)
P 		548.6
(1.1)
P 		371.1
(-3)
P 		261
B 667.2
(3)
P 		482.8
(-0.9)
P 		532.4
(0.1)
P 		385.7
(-3)
P 		282
C 662.5
(3)
P 		437
(-1.2)
P 		486.8
(-0.3)
P 		342.4
(-3)
P 		320
avr 653.9
(3) 		470.7
(-0.8) 		522.6
(0.3) 		366.4
(-3) 		288

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 675
1 2 3 4 5 6 range
A 692.2
(-2.4)
P 		697.2
(-2.3)
P 		712.2
(-2.1)
P 		653.7
(-3)
P 		669.4
(-2.8)
P 		1037.6
(3)
P 		384
B 808.1
(-0.8)
P 		782.3
(-1.2)
P 		789.9
(-1.1)
P 		634
(-3)
P 		722.8
(-1.9)
P 		1118.9
(3)
P 		485
C 660
(-2)
P 		697
(-1.7)
P 		825.3
(-0.5)
P 		740.2
(-1.3)
P 		545.4
(-3)
P 		1220.1
(3)
P 		675
avr 720.1
(-2.1) 		725.5
(-2) 		775.8
(-1.4) 		676
(-2.6) 		645.9
(-3) 		1125.5
(3) 		480
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 176
1 2 3 4 range
A 230.1
(3)
P 		106.8
(-1.2)
P 		143.4
(0)
P 		54.3
(-3)
P 		176
B 212.8
(3)
P 		119.3
(-1.3)
P 		123.4
(-1.1)
P 		82.2
(-3)
P 		131
C 219.3
(3)
P 		125.6
(-0.5)
P 		169.7
(1.2)
P 		57.7
(-3)
P 		162
avr 220.7
(3) 		117.2
(-1) 		145.5
(0.1) 		64.7
(-3) 		156

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 290
1 2 3 4 5 6 range
A 127.6
(-3)
P 		247.9
(2.8)
P 		155.1
(-1.7)
P 		165.8
(-1.2)
P 		202
(0.6)
P 		252.6
(3)
P 		125
B 281.2
(-0.3)
P 		260.4
(-0.8)
P 		191.8
(-2.4)
P 		167.9
(-3)
P 		293.7
(0)
P 		417.2
(3)
P 		249
C 244.6
(-0.8)
P 		212.7
(-1.5)
P 		210.1
(-1.6)
P 		148.9
(-3)
P 		176.5
(-2.4)
P 		408.4
(3)
P 		260
avr 217.8
(-1.3) 		240.3
(-0.6) 		185.7
(-2.3) 		160.9
(-3) 		224.1
(-1.1) 		359.4
(3) 		199
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187357_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8179
Sequence Type Exemplar sequence
Representative Public ID C09F5.2 NCBI
Target Description C09F5.2 /REP_DB=WormBase Gene ID /WP=CE01774 /TR=SW:Q09232 /GB=AAA64507.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064830(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064830 NCBI Caenorhabditis elegans C09F5.2 (C09F5.2) mRNA, complete cds. 11/11 None
SNAP00000035437 ENSEMBL cdna:SNAP chromosome:CEL140:III:652894:654284:1 11/11 None
GENEFINDER00000035441 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:652894:654284:1 11/11 None
C09F5.2 ENSEMBL cdna:known chromosome:CEL140:III:652892:654342:1 gene:C09F5.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:652893-654284(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C09F5.2
Entrez Gene 175221 Entrez gene
SwissProt Q09232 EMBL-EBI
WormBase CE01774 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.13074.1.S1_AT similar to CG11430-PB, isoform B cfa
DROSOPHILA_2:1625514_S_AT dm
DROSGENOME1:141803_AT dm
DROSOPHILA_2:1637534_AT dm
CHICKEN:GGAAFFX.12032.1.S1_S_AT similar to hypothetical protein FLJ14466 gga
HU35KSUBC:RC_AA402823_AT hypothetical protein FLJ14466 hs
U133_X3P:HS.55148.0.A1_3P_AT hypothetical protein FLJ14466 hs
HG-U133_PLUS_2:226531_AT hypothetical protein FLJ14466 hs
HG-U133B:226531_AT hypothetical protein FLJ14466 hs
HG-U95B:46098_AT hypothetical protein FLJ14466 hs
MOE430A:1424989_AT RIKEN cDNA D730049H07 gene mm
MOUSE430A_2:1424989_AT RIKEN cDNA D730049H07 gene mm
MOUSE430_2:1424989_AT RIKEN cDNA D730049H07 gene mm
MOE430A:1424990_AT RIKEN cDNA D730049H07 gene mm
MOUSE430A_2:1424990_AT RIKEN cDNA D730049H07 gene mm
MOUSE430_2:1424990_AT RIKEN cDNA D730049H07 gene mm
MU19KSUBB:TC33833_AT RIKEN cDNA D730049H07 gene, mRNA (cDNA clone MGC:28390 IMAGE:4022503) mm
RG-U34A:RC_AA800218_AT similar to hypothetical protein FLJ14466 (predicted) rn
RAE230A:1390031_AT similar to hypothetical protein FLJ14466 (predicted) rn
RAT230_2:1390031_AT similar to hypothetical protein FLJ14466 (predicted) rn
Protein Similarities
Method ID Description E-Value
blast AAA64507 Hypothetical protein C09F5.2 [Caenorhabditis elegans] ref|NP_497231.1| C09F5.2 [Caenorhabditis elegans] sp|Q09232|YQ22_CAEEL Hypothetical protein C09F5.2 in chromosome III 1.0E-137
blast CAE69215 Hypothetical protein CBG15255 [Caenorhabditis briggsae] 1.0E-113
blast XP_780791 PREDICTED: similar to CG11430-PB, isoform B [Strongylocentrotus purpuratus] 5.0E-21
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497231.1 3 147-166,197-219,229-251
C09F5.2 3 147-166,197-219,229-251
SNAP00000035437 3 147-166,197-219,229-251
GENEFINDER00000035441 3 147-166,197-219,229-251
Sequence
Target Sequence 
>C. ELEGANS:187357_AT
gaacgtcagcacttctggcaggatttgcaatggtatgccttgtcgagttacagtatgacc
agtcgactccaaagccacttctaattgtgttgggcgttgtgacgagtttgttggtgtctg
tgcatttgctggctctcatgatgtccacgtgtattctaccgtatatggaagctactggat
gtacacaggattctccgcatattaagttgaagttctacattgatctgtcatggctcttct
ccacctgcatcggtcttctgctcttcctcgtcgaaatcggtgtcatcttctatgtaaagt
tcacggcagtcggttatccaactgcaggatatatcacaactgcaatgctggtgcctgttg
gagttgtattcgtg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAACGTCAGCACTTCTGGCAGGATT 8 349 374 Antisense
GCAATGGTATGCCTTGTCGAGTTAC 499 319 400 Antisense
TCGACTCCAAAGCCACTTCTAATTG 652 599 436 Antisense
TGTTGGTGTCTGTGCATTTGCTGGC 162 563 482 Antisense
ATTTGCTGGCTCTCATGATGTCCAC 545 17 497 Antisense
GGATGTACACAGGATTCTCCGCATA 298 515 550 Antisense
TACATTGATCTGTCATGGCTCTTCT 122 645 589 Antisense
CTCTTCCTCGTCGAAATCGGTGTCA 415 229 634 Antisense
AAGTTCACGGCAGTCGGTTATCCAA 364 155 670 Antisense
CACAACTGCAATGCTGGTGCCTGTT 180 195 708 Antisense
GGTGCCTGTTGGAGTTGTATTCGTG 241 501 723 Antisense
Sequence Source Affymetrix Proprietary Database