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[187347_at] 7TM chemoreceptor, sra family

Description B0304.6 /REP_DB=WormBase Gene ID /WP=CE02428 /TR=SW:Q10935 /GB=AAK31390.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 44      4grp_total > 0      6grp_cgc > 45      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 121
1 2 3 4 range
A 33.7
(-3)
A 		33.5
(-3)
A 		56.1
(-1.4)
A 		115.9
(3)
P 		82
B 40.2
(-1.7)
P 		24.8
(-3)
A 		58.5
(-0.2)
A 		97.9
(3)
M 		73
C 65.3
(-1.5)
A 		40.8
(-2.8)
A 		37.7
(-3)
A 		145.3
(3)
P 		108
avr 46.4
(-2.1) 		33
(-3) 		50.8
(-1.8) 		119.7
(3) 		87

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 54
1 2 3 4 5 6 range
A 55.7
(2.7)
A 		36
(0.5)
A 		58.5
(3)
P 		5.1
(-3)
A 		51.1
(2.2)
P 		40.2
(0.9)
A 		53
B 52.5
(3)
A 		35.6
(-1.6)
P 		43.9
(0.7)
A 		30.5
(-3)
A 		35.2
(-1.7)
A 		40.4
(-0.3)
P 		22
C 8.9
(-3)
A 		58.9
(3)
P 		47.2
(1.6)
P 		24
(-1.2)
A 		12.6
(-2.6)
A 		22.1
(-1.4)
A 		50
avr 39
(0.8) 		43.5
(1.7) 		49.9
(3) 		19.9
(-3) 		33
(-0.4) 		34.2
(-0.1) 		30
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18
1 2 3 4 range
A 12.3
(0.1)
A 		7.3
(-3)
A 		14.7
(1.6)
A 		17
(3)
P 		10
B 12.8
(-0.3)
P 		6.1
(-3)
A 		13.6
(0)
A 		20.9
(3)
M 		15
C 21.6
(1.6)
A 		11.7
(-3)
A 		13.1
(-2.3)
A 		24.5
(3)
P 		13
avr 15.6
(0.5) 		8.4
(-3) 		13.8
(-0.4) 		20.8
(3) 		12

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 17
1 2 3 4 5 6 range
A 10.3
(0.8)
A 		12.8
(1.9)
A 		12.7
(1.9)
P 		1.3
(-3)
A 		15.4
(3)
P 		9.8
(0.6)
A 		14
B 18.3
(3)
A 		11.9
(-0.8)
P 		10.7
(-1.5)
A 		8.1
(-3)
A 		14.3
(0.6)
A 		15.1
(1.1)
P 		10
C 3.3
(-3)
A 		18
(3)
P 		12
(0.6)
P 		4.8
(-2.4)
A 		4.1
(-2.7)
A 		7.4
(-1.3)
A 		15
avr 10.6
(0.7) 		14.2
(3) 		11.8
(1.5) 		4.7
(-3) 		11.3
(1.2) 		10.8
(0.9) 		10
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187347_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4180
Sequence Type Exemplar sequence
Representative Public ID B0304.6 NCBI
Target Description B0304.6 /REP_DB=WormBase Gene ID /WP=CE02428 /TR=SW:Q10935 /GB=AAK31390.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062401
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062401 NCBI Caenorhabditis elegans Serpentine Receptor, class A (alpha) family member (sra-33) (sra-33) mRNA, complete cds. None
B0304.6 ENSEMBL cdna:known chromosome:CEL140:II:4534737:4537228:-1 gene:B0304.6 None
GENEFINDER00000018317 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:4534501:4537228:-1 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4533589-4538712(-) 100.0 100.0
Public Domain and Genome References
Gene Title 7TM chemoreceptor, sra family
Gene Symbol sra-33
Ensembl B0304.6
Entrez Gene 181920 Entrez gene
SwissProt Q10935 EMBL-EBI
WormBase CE34634 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7606 sensory perception of chemical stimulus inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4888 transmembrane receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK31390 Serpentine receptor, class a (alpha) protein 33 [Caenorhabditis elegans] ref|NP_494802.3| Serpentine Receptor, class A (alpha) family member (sra-33) [Caenorhabditis elegans] sp|Q10935|SRA33_CAEEL Serpentine receptor class alpha-33 (Protein sra-33) 0.0
blast CAE67862 Hypothetical protein CBG13454 [Caenorhabditis briggsae] 1.0E-60
blast AAP86618 Serpentine receptor, class a (alpha) protein 32 [Caenorhabditis elegans] ref|NP_494801.2| Serpentine Receptor, class A (alpha) family member (sra-32) [Caenorhabditis elegans] sp|Q10934|SRA32_CAEEL Serpentine receptor class alpha-32 (Protein sra-32) 3.0E-49
blast AAP86618 Serpentine receptor, class a (alpha) protein 32 [Caenorhabditis elegans] ref|NP_494801.2| Serpentine Receptor, class A (alpha) family member (sra-32) [Caenorhabditis elegans] sp|Q10934|SRA32_CAEEL Serpentine receptor class alpha-32 (Protein sra-32) 2.0E-48
Protein Domains
Database ID Description E-Value
Pfam IPR000344 EMBL-EBI Nematode chemoreceptor, Sra 1.1E-4
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494802.3 6 20-42,57-79,138-160,185-207,233-255,270-292
B0304.6 6 20-42,57-79,138-160,185-207,233-255,270-292
GENEFINDER00000018317 6 20-42,57-79,138-160,185-207,233-255,270-292
Sequence
Target Sequence 
>C. ELEGANS:187347_AT
gcaagtacggtattcacagctcaacaacatttccttgggccaattcatggaatgaggact
acaagttgcttccgggaatccgatgttgttttggacttgaaaacagagcatataattcca
tatcttgcaatttgtcttacctcaatagtttgctctttgcttctcatcatttatgtgaag
aagactcaaaaaacaaaaacgtacgacattgagtctgaatatacaaaaaaggaagccgta
gtttcaagtatttccgtggcaattctgggaattattcaactggttttattttgcttctat
gacacttttcttacgattttcgcaaaacttactgctgaaaatccaaatgttcatgataca
aatattattggatggttttatacatctccattgaatgcaattatttctcccactgccgaa
atattaattttcgagaaaacaatatgttgtttcaaaattcaattatctcaccagtcgccg
ttattttcacttgcgtgcttcgtgggttttaggaaacaagtcgagtgttgtagcttgcag
gca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCAAGTACGGTATTCACAGCTCAAC 394 321 499 Antisense
CAGCTCAACAACATTTCCTTGGGCC 138 203 515 Antisense
GAGGACTACAAGTTGCTTCCGGGAA 139 403 552 Antisense
CAATAGTTTGCTCTTTGCTTCTCAT 41 181 641 Antisense
CAAGTATTTCCGTGGCAATTCTGGG 628 181 743 Antisense
TGACACTTTTCTTACGATTTTCGCA 317 571 798 Antisense
GAATGCAATTATTTCTCCCACTGCC 525 331 891 Antisense
AAATTCAATTATCTCACCAGTCGCC 146 117 953 Antisense
CAGTCGCCGTTATTTTCACTTGCGT 611 207 970 Antisense
TCACTTGCGTGCTTCGTGGGTTTTA 241 625 985 Antisense
AGTCGAGTGTTGTAGCTTGCAGGCA 270 61 1017 Antisense
Sequence Source Affymetrix Proprietary Database