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[187345_at]

Description Y57E12AL.1 /REP_DB=WormBase Gene ID /WP=CE26191 /TR=Q9N335 /GB=AAF59616.2 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 89      6grp_high1_10_10 > 99      4grp_highest_8_8 > 38      4grp_high_8_8 > 03      6grp_highest_nonorm > 58      6grp_total > 95      4grp_total > 06      6grp_highest_sub > In      6grp_total_sub > Increase      6grp_rule_based > S5      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2044
1 2 3 4 range
A 2254.5
(1.4)
P 		2356.4
(3)
P 		2256.7
(1.4)
P 		1979.9
(-3)
P 		377
B 2600.1
(-1)
P 		2067.3
(-3)
P 		2284
(-2.2)
P 		3638.6
(3)
P 		1571
C 2340.4
(1.3)
P 		1594.7
(-3)
P 		1937.1
(-1)
P 		2637.8
(3)
P 		1043
avr 2398.3
(0.2) 		2006.1
(-3) 		2159.3
(-1.8) 		2752.1
(3) 		746

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1335
1 2 3 4 5 6 range
A 1953.6
(-3)
P 		2216.8
(-1.6)
P 		2135.9
(-2)
P 		2387.8
(-0.6)
P 		3044
(3)
P 		2834.4
(1.8)
P 		1090
B 1830.8
(-2.3)
P 		2209.2
(-0.2)
P 		1708.9
(-3)
P 		2454.6
(1.1)
P 		2794.8
(3)
P 		2718.2
(2.6)
P 		1086
C 1849.6
(-3)
P 		1888.3
(-2.8)
P 		2544.4
(0.9)
P 		2294
(-0.5)
P 		2916.1
(3)
P 		2640.7
(1.5)
P 		1067
avr 1878
(-3) 		2104.8
(-1.7) 		2129.7
(-1.5) 		2378.8
(-0.1) 		2918.3
(3) 		2731.1
(1.9) 		1040
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 539
1 2 3 4 range
A 820.9
(3)
P 		511.5
(-0.5)
P 		590
(0.4)
P 		289.8
(-3)
P 		531
B 829.1
(3)
P 		510.6
(-3)
P 		529.3
(-2.6)
P 		775.4
(2)
P 		319
C 774.7
(3)
P 		458.5
(-2.8)
P 		675.2
(1.2)
P 		444.8
(-3)
P 		330
avr 808.2
(3) 		493.5
(-3) 		598.2
(-1) 		503.3
(-2.8) 		315

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 775
1 2 3 4 5 6 range
A 360.1
(-3)
P 		788.4
(1.6)
P 		465.2
(-1.9)
P 		605.6
(-0.4)
P 		918.5
(3)
P 		690.1
(0.5)
P 		558
B 637
(-1.2)
P 		735.4
(-0.3)
P 		415
(-3)
P 		650.1
(-1)
P 		1135.5
(3)
P 		1013.6
(2)
P 		721
C 685.6
(-0.2)
P 		576.2
(-1.6)
P 		647.6
(-0.7)
P 		461.3
(-3)
P 		943.6
(3)
P 		884
(2.3)
P 		482
avr 560.9
(-2.4) 		700
(-0.7) 		509.3
(-3) 		572.3
(-2.2) 		999.2
(3) 		862.6
(1.3) 		490
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187345_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11764
Sequence Type Exemplar sequence
Representative Public ID Y57E12AL.1 NCBI
Target Description Y57E12AL.1 /REP_DB=WormBase Gene ID /WP=CE26191 /TR=Q9N335 /GB=AAF59616.2 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171479(11), NM_171930(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171479 NCBI Caenorhabditis elegans Y57E12AL.1a (Y57E12AL.1) mRNA, complete cds. 11/11 None
NM_171930 NCBI Caenorhabditis elegans Y57E12AL.1b (Y57E12AL.1) mRNA, complete cds. 11/11 None
Y57E12AL.1a.2 ENSEMBL cdna:known chromosome:CEL140:V:7063403:7065107:1 gene:Y57E12AL.1 11/11 None
Y57E12AL.1a.1 ENSEMBL cdna:known chromosome:CEL140:V:7063404:7065288:1 gene:Y57E12AL.1 11/11 None
Y57E12AL.1b ENSEMBL cdna:known chromosome:CEL140:V:7063408:7065280:1 gene:Y57E12AL.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000015457 4/11 Cross Hyb Matching Probes None
GENEFINDER00000015461 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:7063315-7065108(+) 100.0 100.0
Public Domain and Genome References
Ensembl Y57E12AL.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAL27256 Hypothetical protein Y57E12AL.1a [Caenorhabditis elegans] ref|NP_741561.1| Y57E12AL.1a [Caenorhabditis elegans] 0.0
blast CAE71996 Hypothetical protein CBG19072 [Caenorhabditis briggsae] 0.0
blast AAL27257 Hypothetical protein Y57E12AL.1b [Caenorhabditis elegans] ref|NP_741562.1| Y57E12AL.1b [Caenorhabditis elegans] 0.0
blast AAL27256 Hypothetical protein Y57E12AL.1a [Caenorhabditis elegans] ref|NP_741561.1| Y57E12AL.1a [Caenorhabditis elegans] 1.0E-175
Protein Domains
Database ID Description E-Value
Pfam IPR005016 EMBL-EBI TMS membrane protein/tumour differentially expressed protein 1.0E-126
Pfam IPR005016 EMBL-EBI TMS membrane protein/tumour differentially expressed protein 2.2E-44
Pfam IPR005016 EMBL-EBI TMS membrane protein/tumour differentially expressed protein 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_741561.1 11 5-27,40-62,92-114,127-146,156-175,196-218,233-255,262-284,316-338,388-410,430-452
NP_741562.1 9 5-27,40-62,103-125,137-159,169-191,198-220,252-274,318-340,360-382
Y57E12AL.1a.2 11 5-27,40-62,92-114,127-146,156-175,196-218,233-255,262-284,316-338,388-410,430-452
Y57E12AL.1a.1 11 5-27,40-62,92-114,127-146,156-175,196-218,233-255,262-284,316-338,388-410,430-452
Y57E12AL.1b 9 5-27,40-62,103-125,137-159,169-191,198-220,252-274,318-340,360-382
Sequence
Target Sequence 
>C. ELEGANS:187345_AT
ggctccagcggttcaagaagtctcaccaagatcgggtcttgttcaagcagttatgatcac
cggatacgttatgtatctaacatgggctgctttgatcaacaacccagataaggcctgtaa
tccttcactcatcagcattttcactggaaacagcactgatccatcacacaaaaccgacga
aggacattatggaattccacttccagctcagtcgatcgtctctctgttcctctggtttgc
gtgtcttctatatgcatcgatccgcaattcgtcgaatacctcactcggaaaaattactgg
aggaggaaataccagcgatgaagctattcaactaagttcgtctttgaatggtgctgatga
tgcagaatcacaaaactccaaacgagtatacgataacgaagaagaaggcgtagcttattc
ctatagtttcttccacttcatgtttgccctggcttccctctacgttatgatgacactgac
ttcgtggtacaaaccatctaacgatttgtcccatttgaattcaaatatggcatctgtctg
ggtgaaaatagtttcatcgtgggtgtgtgttgctctttactgctggacgttggttgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGCTCCAGCGGTTCAAGAAGTCTCA 669 539 858 Antisense
AGAAGTCTCACCAAGATCGGGTCTT 33 75 873 Antisense
GGGCTGCTTTGATCAACAACCCAGA 494 493 941 Antisense
TAAGGCCTGTAATCCTTCACTCATC 472 637 966 Antisense
GAAACAGCACTGATCCATCACACAA 2 357 1004 Antisense
GCGTGTCTTCTATATGCATCGATCC 180 293 1096 Antisense
CAATTCGTCGAATACCTCACTCGGA 146 177 1122 Antisense
ATTCCTATAGTTTCTTCCACTTCAT 177 9 1274 Antisense
GCTTCCCTCTACGTTATGATGACAC 69 307 1309 Antisense
CCATCTAACGATTTGTCCCATTTGA 432 271 1351 Antisense
CTCTTTACTGCTGGACGTTGGTTGC 92 231 1430 Antisense
Sequence Source Affymetrix Proprietary Database