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[187342_s_at]

Description F55F8.5 /REP_DB=WormBase Gene ID /WP=CE11196 /TR=SW:P91343 /GB=AAB37809.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 68      6grp_high1_10_10 > 85      4grp_highest_8_8 > 01      4grp_high_8_8 > 53      6grp_highest_nonorm > 83      6grp_total > 76      4grp_total > 58      6grp_highest_sub > In      6grp_cgc > 57      6grp_total_sub > Increase      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 284
1 2 3 4 range
A 513.7
(-3)
P 		682.5
(3)
P 		588.2
(-0.4)
P 		521.5
(-2.7)
P 		169
B 487.7
(-0.5)
P 		597.9
(3)
P 		555.6
(1.7)
P 		408.6
(-3)
P 		189
C 600
(0.2)
P 		647.5
(1.6)
P 		692.9
(3)
P 		491.3
(-3)
P 		202
avr 533.8
(-0.9) 		642.6
(3) 		612.2
(1.9) 		473.8
(-3) 		169

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 464
1 2 3 4 5 6 range
A 274.1
(-3)
P 		332.4
(-1.7)
P 		493.7
(2)
P 		417.2
(0.3)
P 		413.5
(0.2)
P 		538.2
(3)
P 		264
B 287.6
(-3)
P 		508.1
(0.4)
P 		375.8
(-1.6)
P 		467.7
(-0.2)
P 		481.2
(-0)
P 		677.3
(3)
P 		390
C 274.8
(-3)
P 		518.1
(0.2)
P 		323.5
(-2.4)
P 		553.5
(0.6)
P 		378.5
(-1.7)
P 		738.1
(3)
P 		463
avr 278.8
(-3) 		452.9
(-0.2) 		397.7
(-1.1) 		479.5
(0.2) 		424.4
(-0.7) 		651.2
(3) 		372
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 165
1 2 3 4 range
A 187
(3)
P 		148.2
(0.9)
P 		153.8
(1.2)
P 		76.3
(-3)
P 		111
B 155.5
(3)
P 		147.7
(2.3)
P 		128.7
(0.6)
P 		87.1
(-3)
P 		68
C 198.6
(1.4)
P 		186.2
(0.9)
P 		241.5
(3)
P 		82.8
(-3)
P 		159
avr 180.4
(3) 		160.7
(1.8) 		174.7
(2.7) 		82.1
(-3) 		98

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 202
1 2 3 4 5 6 range
A 50.5
(-3)
P 		118.2
(2)
P 		107.5
(1.2)
P 		105.8
(1.1)
P 		124.8
(2.5)
P 		131
(3)
P 		81
B 100.1
(-2.7)
P 		169.1
(-0.1)
P 		91.3
(-3)
P 		123.9
(-1.8)
P 		195.5
(0.9)
P 		252.6
(3)
P 		161
C 101.8
(-2.3)
P 		158.1
(-0.2)
P 		82.3
(-3)
P 		111.3
(-1.9)
P 		122.5
(-1.5)
P 		247.1
(3)
P 		165
avr 84.1
(-3) 		148.5
(0.1) 		93.7
(-2.5) 		113.7
(-1.6) 		147.6
(0) 		210.2
(3) 		126
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 187342_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.352
Sequence Type Exemplar sequence
Representative Public ID F55F8.5 NCBI
Target Description F55F8.5 /REP_DB=WormBase Gene ID /WP=CE11196 /TR=SW:P91343 /GB=AAB37809.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059254(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059254 NCBI Caenorhabditis elegans F55F8.5 (F55F8.5) mRNA, complete cds. 11/11 A 
SNAP00000024469 ENSEMBL cdna:SNAP chromosome:CEL140:I:5656069:5657912:-1 11/11 None
GENEFINDER00000024481 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:5656069:5657912:-1 11/11 None
F55F8.5.1 ENSEMBL cdna:known chromosome:CEL140:I:5655953:5657926:-1 gene:F55F8.5 11/11 None
F55F8.5.2 ENSEMBL cdna:known chromosome:CEL140:I:5655969:5657920:-1 gene:F55F8.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:5656068-5657912(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol tag-345
Ensembl F55F8.5
Entrez Gene 172226 Entrez gene
SwissProt P91343 EMBL-EBI
WormBase CE11196 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:246559_AT transducin family protein / WD-40 repeat family protein at
CANINE:1588389_AT similar to WD-repeat protein 12 (YTM1 homolog) cfa
CANINE_2:CFA.10515.1.S1_AT similar to WD-repeat protein 12 (YTM1 homolog) cfa
CANINE_2:CFAAFFX.19429.1.S1_S_AT similar to WD-repeat protein 12 (YTM1 homolog) cfa
DROSOPHILA_2:1630344_AT dm
DROSGENOME1:146191_AT dm
CHICKEN:GGA.12599.1.S1_S_AT WD repeat domain 12 gga
CHICKEN:GGA.12630.1.S1_S_AT WD repeat domain 12 gga
CHICKEN:GGAAFFX.21435.1.S1_X_AT WD repeat domain 12 gga
HU35KSUBC:RC_AA278650_AT WD repeat domain 12 hs
HG-U133A:218512_AT WD repeat domain 12 hs
HG-U133_PLUS_2:218512_AT WD repeat domain 12 hs
HG-U133A_2:218512_AT WD repeat domain 12 hs
U133_X3P:G8922736_3P_AT WD repeat domain 12 hs
HG-U95C:62554_AT WD repeat domain 12 hs
MG-U74BV2:163787_AT WD repeat domain 12 mm
MG-U74BV2:163097_AT WD repeat domain 12 mm
MOE430A:1448646_AT WD repeat domain 12 mm
MOUSE430A_2:1448646_AT WD repeat domain 12 mm
MOUSE430_2:1448646_AT WD repeat domain 12 mm
MOE430A:1417270_AT WD repeat domain 12 mm
MOUSE430A_2:1417270_AT WD repeat domain 12 mm
MOUSE430_2:1417270_AT WD repeat domain 12 mm
MU19KSUBC:TC37475_AT WD repeat domain 12 mm
MU19KSUBA:TC20984_AT WD repeat domain 12 (Wdr12) mm
RG-U34B:RC_AA848530_AT WD repeat domain 12 rn
RAE230A:1372218_AT WD repeat domain 12 rn
RAT230_2:1372218_AT WD repeat domain 12 rn
Protein Similarities
Method ID Description E-Value
blast AAB37809 Temporarily assigned gene name protein 345 [Caenorhabditis elegans] ref|NP_491655.1| F55F8.5 [Caenorhabditis elegans] sp|P91343|YM3M_CAEEL Hypothetical WD-repeat protein F55F8.5 in chromosome I 0.0
blast CAE68942 Hypothetical protein CBG14922 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 3.1E-9
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 4.2E-7
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.26
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 5.8E-7
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 2.0E-8
Sequence
Target Sequence 
>C. ELEGANS:187342_S_AT
ggaacttggatgcatctgatgaagcaacaacatatgagaaagaagaagaagaagaaagtg
cgaagaaacgccgaaagaaagaaaccaaaactaaagttccgatggttacgattggagggc
atcgtgacaaagtatccgcagtcgaatggtgtccatggaattctggacacgcattttcgt
gctcgtgggatcatactattgttgaatgggatttggaattagctggtgaaatttcgcgga
tcaaaggacccaaatcattcacaagcattgatcttcatccgaagagtgatcttctcatct
ccagttgtactgatgctattccaagacttta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAACTTGGATGCATCTGATGAAGC 571 529 725 Antisense
GTTCCGATGGTTACGATTGGAGGGC 78 441 820 Antisense
GGGCATCGTGACAAAGTATCCGCAG 366 493 841 Antisense
AGTATCCGCAGTCGAATGGTGTCCA 423 63 855 Antisense
TGGACACGCATTTTCGTGCTCGTGG 571 547 888 Antisense
GTGCTCGTGGGATCATACTATTGTT 633 479 903 Antisense
GAAATTTCGCGGATCAAAGGACCCA 620 361 952 Antisense
AGGACCCAAATCATTCACAAGCATT 245 55 969 Antisense
CACAAGCATTGATCTTCATCCGAAG 104 195 984 Antisense
AAGAGTGATCTTCTCATCTCCAGTT 78 155 1006 Antisense
GTACTGATGCTATTCCAAGACTTTA 448 459 1031 Antisense
Sequence Source Affymetrix Proprietary Database