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[187340_s_at]

Description F22B7.13 /REP_DB=WormBase Gene ID /WP=CE24910 /GEN=ags-3.2 /GB=AAK68341.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 87      6grp_high1_10_10 > 78      4grp_highest_8_8 > 10      4grp_high_8_8 > 54      6grp_highest_nonorm > 83      6grp_total > 78      4grp_total > 57      6grp_highest_sub > Da      6grp_cgc > 49      6grp_high1_sub > Cu      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 456
1 2 3 4 range
A 353.7
(-3)
P 		539.9
(0.8)
P 		647.3
(3)
P 		402.3
(-2)
P 		294
B 341
(-0.8)
P 		551
(2.3)
P 		597.4
(3)
P 		191.1
(-3)
P 		406
C 433.7
(-0.1)
P 		568.1
(2.8)
P 		579.5
(3)
P 		295.1
(-3)
P 		284
avr 376.1
(-1.5) 		553
(1.9) 		608.1
(3) 		296.2
(-3) 		312

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 513
1 2 3 4 5 6 range
A 377.2
(-2.5)
P 		359.9
(-3)
P 		365.5
(-2.9)
P 		391.8
(-2.2)
P 		490.1
(0.4)
P 		590.5
(3)
P 		231
B 476.1
(-0.6)
P 		310.5
(-3)
P 		366.2
(-2.2)
P 		441.9
(-1.1)
P 		503.4
(-0.2)
P 		730.9
(3)
P 		420
C 364.1
(-2.4)
P 		397.4
(-2)
P 		314.3
(-3)
P 		355.3
(-2.5)
P 		396.6
(-2)
P 		823.2
(3)
P 		509
avr 405.8
(-2.1) 		355.9
(-2.9) 		348.7
(-3) 		396.3
(-2.2) 		463.4
(-1.1) 		714.9
(3) 		366
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 161
1 2 3 4 range
A 128.8
(0.8)
P 		117.2
(0.2)
P 		169.2
(3)
P 		58.9
(-3)
P 		110
B 108.7
(1.2)
P 		136.1
(2.9)
P 		138.4
(3)
P 		40.7
(-3)
P 		98
C 143.6
(0.7)
P 		163.3
(1.5)
P 		202
(3)
P 		49.8
(-3)
P 		152
avr 127
(0.9) 		138.9
(1.5) 		169.9
(3) 		49.8
(-3) 		120

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 206
1 2 3 4 5 6 range
A 69.5
(-3)
P 		128
(1.5)
P 		79.6
(-2.2)
P 		99.4
(-0.7)
P 		147.9
(3)
P 		143.8
(2.7)
P 		78
B 165.7
(-0.5)
P 		103.3
(-2.5)
P 		88.9
(-3)
P 		117
(-2.1)
P 		204.5
(0.8)
P 		272.6
(3)
P 		184
C 134.9
(-1.1)
P 		121.3
(-1.5)
P 		80
(-2.8)
P 		71.5
(-3)
P 		128.3
(-1.3)
P 		275.6
(3)
P 		204
avr 123.4
(-1.4) 		117.5
(-1.6) 		82.8
(-3) 		96
(-2.5) 		160.2
(0.1) 		230.7
(3) 		148
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 187340_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6019
Sequence Type Exemplar sequence
Representative Public ID F22B7.13 NCBI
Target Description F22B7.13 /REP_DB=WormBase Gene ID /WP=CE24910 /GEN=ags-3.2 /GB=AAK68341.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066499(11), NM_066665(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066499 NCBI Caenorhabditis elegans G Protein Regulator family member (gpr-1) (gpr-1) mRNA, complete cds. 11/11 None
NM_066665 NCBI Caenorhabditis elegans G Protein Regulator family member (gpr-2) (gpr-2) mRNA, complete cds. 11/11 A 
SNAP00000012406 ENSEMBL cdna:SNAP chromosome:CEL140:III:9391305:9393037:-1 11/11 None
GENEFINDER00000012412 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:9391305:9393037:-1 11/11 None
SNAP00000003831 ENSEMBL cdna:SNAP chromosome:CEL140:III:8628837:8633237:1 11/11 None
GENEFINDER00000003840 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:8628860:8633237:1 9/11 None
F22B7.13 ENSEMBL cdna:known chromosome:CEL140:III:8628838:8630680:1 gene:F22B7.13 11/11 None
C38C10.4 ENSEMBL cdna:known chromosome:CEL140:III:9391290:9393062:-1 gene:C38C10.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8628854-8630585(+) 100.0 100.0
chrIII:9391300-9393033(-) 97.78 97.78
Public Domain and Genome References
Gene Title

Gene Symbol gpr-2
gpr-1
Ensembl F22B7.13
C38C10.4
Entrez Gene 176210 Entrez gene
176318 Entrez gene
SwissProt Q03569 EMBL-EBI
Q95QJ7 EMBL-EBI
WormBase CE00107 Wormbase
CE24910 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7165 signal transduction inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5096 GTPase activator activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK68341 G protein regulator protein 1 [Caenorhabditis elegans] ref|NP_498900.1| G Protein Regulator family member (gpr-1) [Caenorhabditis elegans] sp|Q95QJ7|GPR1_CAEEL G-protein regulator 0.0
blast CAA79548 Hypothetical protein C38C10.4 [Caenorhabditis elegans] ref|NP_499066.1| G Protein Regulator family member (gpr-2) [Caenorhabditis elegans] sp|Q03569|GPR2_CAEEL G-protein regulator 0.0
Protein Domains
Database ID Description E-Value
scop a.2.3.Chaperone J-domain All alpha proteins; Long alpha-hairpin; Chaperone J-domain; Chaperone J-domain 2.99999998801259E-12
scop a.2.3.Chaperone J-domain All alpha proteins; Long alpha-hairpin; Chaperone J-domain; Chaperone J-domain 3.00000015723206E-23
Pfam IPR003109 EMBL-EBI GoLoco 1.6E-9
Pfam IPR001623 EMBL-EBI Heat shock protein DnaJ, N-terminal 1.3E-21
Pfam IPR002939 EMBL-EBI Chaperone DnaJ, C-terminal 1.9E-60
Pfam IPR003109 EMBL-EBI GoLoco 1.6E-9
Pfam IPR001305 EMBL-EBI DnaJ central domain (CXXCXGXG) 1.1E-40
Pfam IPR001623 EMBL-EBI Heat shock protein DnaJ, N-terminal 1.1E-33
Pfam IPR002939 EMBL-EBI Chaperone DnaJ, C-terminal 1.9E-60
Pfam IPR001305 EMBL-EBI DnaJ central domain (CXXCXGXG) 1.1E-40
Sequence
Target Sequence 
>C. ELEGANS:187340_S_AT
gaaaaagacgccaagctgcttattttatcatcttggggagtgttctgaccatcaaccaaa
acagtttcaaactgttctacgagcagatcgacgtggcgatgaatcaagagagaagcgaaa
cggcaactgatcaagatgtatgcctcgcaattgattcgtctcctgatccgacatcctcga
atgacatgattaataagttcgtcgtcgaactggagcacgcaacaaatgtggaaacctggg
aaatgattgtcaacggaatcattgacgaccagaagaaaccagtggcgatcgaaaagaaag
agaacgaggaacccgtagacatgatggatctcattttcagtatgagctcacgtatggatg
atcaaagaactgaactgcctgctgccagattcattccgcctcgtccagtgtcatcggcat
cgaaaaagactacaaagagtcacagaatcctccctggactccgtgccaattggacaaaag
tgcagtcgatgaagttcgatggtcacacaatgaataggatcctgaagaggtcgaagaaaa
gcaaatcgtcattggattctacaaattcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAAAAGACGCCAAGCTGCTTATTT 193 355 971 Antisense
CTGCTTATTTTATCATCTTGGGGAG 579 235 986 Antisense
TGGGGAGTGTTCTGACCATCAACCA 614 553 1004 Antisense
GTTCTACGAGCAGATCGACGTGGCG 649 443 1044 Antisense
GAAGCGAAACGGCAACTGATCAAGA 396 341 1082 Antisense
CTGATCCGACATCCTCGAATGACAT 614 237 1133 Antisense
GGAACCCGTAGACATGATGGATCTC 278 531 1278 Antisense
TCGTCCAGTGTCATCGGCATCGAAA 534 605 1371 Antisense
CTGGACTCCGTGCCAATTGGACAAA 330 241 1424 Antisense
GAAGTTCGATGGTCACACAATGAAT 356 337 1461 Antisense
ATCGTCATTGGATTCTACAAATTCG 501 35 1515 Antisense
Sequence Source Affymetrix Proprietary Database