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[187319_at]

Description C27F2.7 /REP_DB=WormBase Gene ID /WP=CE01174 /TR=SW:Q18262 /GB=AAA81427.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 96      6grp_total > 87      4grp_total > 12      6grp_high1_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 219
1 2 3 4 range
A 162.3
(-1)
P 		141.5
(-1.5)
A 		88.5
(-3)
A 		307
(3)
P 		219
B 167.3
(2)
P 		135.9
(-0.2)
A 		96.8
(-3)
A 		182
(3)
P 		85
C 146.4
(-2)
P 		154.8
(-1.7)
A 		116.3
(-3)
P 		298.6
(3)
P 		182
avr 158.7
(-0.8) 		144.1
(-1.4) 		100.5
(-3) 		262.5
(3) 		162

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 328
1 2 3 4 5 6 range
A 114.5
(-3)
M 		132.3
(-2.6)
P 		175.2
(-1.7)
P 		190.9
(-1.3)
P 		293.9
(0.9)
P 		387.9
(3)
P 		273
B 158.2
(-2.1)
P 		121.9
(-3)
P 		157.8
(-2.1)
P 		163.9
(-2)
P 		231.1
(-0.3)
P 		367.6
(3)
P 		246
C 134.7
(-3)
P 		156.9
(-2.6)
P 		194.2
(-1.8)
P 		226.7
(-1.2)
P 		270.7
(-0.3)
P 		442.1
(3)
P 		307
avr 135.8
(-3) 		137
(-3) 		175.7
(-2.1) 		193.8
(-1.7) 		265.2
(-0.1) 		399.2
(3) 		263
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 37
1 2 3 4 range
A 59.1
(3)
P 		30.7
(-1.7)
A 		23.1
(-3)
A 		44.9
(0.6)
P 		36
B 53.3
(3)
P 		33.6
(-0.8)
A 		22.4
(-3)
A 		38.8
(0.2)
P 		31
C 48.4
(1.8)
P 		44.5
(-0.6)
A 		40.5
(-3)
P 		50.3
(3)
P 		10
avr 53.6
(3) 		36.3
(-1.2) 		28.7
(-3) 		44.7
(0.9) 		25

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 127
1 2 3 4 5 6 range
A 21.1
(-3)
M 		47.1
(-0.9)
P 		38.1
(-1.6)
P 		48.4
(-0.8)
P 		88.7
(2.5)
P 		94.4
(3)
P 		73
B 55
(-2)
P 		40.6
(-2.9)
P 		38.3
(-3)
P 		43.4
(-2.7)
P 		93.9
(0.4)
P 		137.1
(3)
P 		99
C 49.9
(-2.7)
P 		47.9
(-2.9)
P 		49.4
(-2.8)
P 		45.6
(-3)
P 		87.6
(-0.5)
P 		148
(3)
P 		102
avr 42
(-3) 		45.2
(-2.8) 		41.9
(-3) 		45.8
(-2.7) 		90.1
(0.4) 		126.5
(3) 		85
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187319_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7129
Sequence Type Exemplar sequence
Representative Public ID C27F2.7 NCBI
Target Description C27F2.7 /REP_DB=WormBase Gene ID /WP=CE01174 /TR=SW:Q18262 /GB=AAA81427.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065655(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065655 NCBI Caenorhabditis elegans C27F2.7 (C27F2.7) mRNA, complete cds. 11/11 None
SNAP00000001015 ENSEMBL cdna:SNAP chromosome:CEL140:III:4980605:4983669:-1 11/11 None
GENEFINDER00000001021 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:4980605:4986835:-1 11/11 None
C27F2.7 ENSEMBL cdna:known chromosome:CEL140:III:4980605:4984436:-1 gene:C27F2.7 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000012147 4/11 Cross Hyb Matching Probes None
GENEFINDER00000012160 4/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4980603-4986653(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C27F2.7
Entrez Gene 259768 Entrez gene
WormBase CE38942 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_498056 C27F2.7 [Caenorhabditis elegans] sp|Q18262|YPI7_CAEEL Hypothetical F-box protein C27F2.7 0.0
blast T15664 hypothetical protein C27F2.7 - Caenorhabditis elegans 0.0
blast AAL02445 Hypothetical protein C27F2.7 [Caenorhabditis elegans] 0.0
blast NP_498057 C27F2.10 [Caenorhabditis elegans] gb|AAL02444.1| Hypothetical protein C27F2.10 [Caenorhabditis elegans] sp|Q95QU0|YPIA_CAEEL Hypothetical protein C27F2.10 in chromosome III 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 9.2E-8
Pfam IPR000717 EMBL-EBI Proteasome component region PCI 9.0E-13
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 9.2E-8
Sequence
Target Sequence 
>C. ELEGANS:187319_AT
attcgaggagttctggcacctttctcaagagctttgacaaagataacattcgaaactaca
gtattcgtcactgattggcttgatgaaattcttcaattacatcgtgagaaccgattgatt
ccattgagccttctgttcactggtggtgctctgacgaaaggtcatcaagtcactggagcc
gatctacgatctattacggagacggaatttgtggattttgtcacgaaacttcagccacat
ttgcaagaagttcagttgagcacttcaagaatcttcaaaatcagcacagatccatctcaa
cttcttggaatgatttcaatgttatcaagctttggaattgtctacgaacgaccaccgctt
cacttttatcatgaagaaatttcaaatgcaatagcgttgtggaaatcagatcctctgtcc
agatgctgtgatgtatacatgcgtcgaccacacgatgtctcttcagaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTCGAGGAGTTCTGGCACCTTTCT 660 1 1609 Antisense
GGCACCTTTCTCAAGAGCTTTGACA 188 535 1623 Antisense
CGATTGATTCCATTGAGCCTTCTGT 141 249 1720 Antisense
TCTGTTCACTGGTGGTGCTCTGACG 395 607 1740 Antisense
AAGTCACTGGAGCCGATCTACGATC 95 157 1775 Antisense
TCACGAAACTTCAGCCACATTTGCA 167 627 1829 Antisense
AGCACAGATCCATCTCAACTTCTTG 281 77 1891 Antisense
ACCACCGCTTCACTTTTATCATGAA 520 85 1959 Antisense
TCAGATCCTCTGTCCAGATGCTGTG 651 623 2014 Antisense
TGATGTATACATGCGTCGACCACAC 151 567 2037 Antisense
TCGACCACACGATGTCTCTTCAGAA 591 601 2052 Antisense
Sequence Source Affymetrix Proprietary Database