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[187318_at]

Description F48E8.1 /REP_DB=WormBase Gene ID /WP=CE01953 /TR=SW:Q09566 /GB=AAC46538.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 02      6grp_high1_10_10 > 01      4grp_high_8_8 > 53      6grp_highest_nonorm > 21      6grp_total > 23      4grp_total > 58      6grp_cgc > 70      6grp_high1_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3294
1 2 3 4 range
A 1178.8
(-1)
P 		3275.6
(3)
P 		3238.8
(2.9)
P 		135.8
(-3)
P 		3140
B 1157.6
(-0.5)
P 		2695.2
(3)
P 		2325.3
(2.2)
P 		82
(-3)
A 		2613
C 1192.3
(-1)
P 		2626.8
(1.6)
P 		3376.1
(3)
P 		109.9
(-3)
M 		3266
avr 1176.2
(-0.8) 		2865.9
(2.8) 		2980.1
(3) 		109.2
(-3) 		2871

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1817
1 2 3 4 5 6 range
A 2164.2
(3)
P 		1170.8
(-0.3)
P 		1195.4
(-0.2)
P 		1205.6
(-0.2)
P 		1001.6
(-0.8)
P 		346.8
(-3)
P 		1817
B 1975.9
(3)
P 		1512.8
(1.2)
P 		1920
(2.8)
P 		1219.1
(-0)
P 		1299.9
(0.3)
P 		462.8
(-3)
P 		1513
C 1777.3
(3)
P 		1454.5
(1.6)
P 		1358.9
(1.2)
P 		1138.3
(0.2)
P 		1041.1
(-0.2)
P 		409
(-3)
P 		1368
avr 1972.5
(3) 		1379.4
(0.7) 		1491.4
(1.2) 		1187.7
(-0) 		1114.2
(-0.3) 		406.2
(-3) 		1566
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1159
1 2 3 4 range
A 429.2
(-0)
P 		711
(2)
P 		846.7
(3)
P 		19.9
(-3)
P 		827
B 369.1
(0.3)
P 		665.7
(3)
P 		538.8
(1.8)
P 		17.5
(-3)
A 		648
C 394.7
(-1.1)
P 		755.2
(0.8)
P 		1176.7
(3)
P 		18.5
(-3)
M 		1158
avr 397.7
(-0.3) 		710.6
(2) 		854.1
(3) 		18.6
(-3) 		836

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 603
1 2 3 4 5 6 range
A 399
(2.7)
P 		416.4
(3)
P 		260.3
(0.2)
P 		305.8
(1)
P 		302.2
(0.9)
P 		84.4
(-3)
P 		332
B 687.5
(3)
P 		503.6
(0.9)
P 		466.2
(0.4)
P 		322.9
(-1.2)
P 		528.1
(1.1)
P 		172.6
(-3)
P 		515
C 658.8
(3)
P 		443.8
(0.5)
P 		345.9
(-0.6)
P 		228.9
(-1.9)
P 		336.9
(-0.7)
P 		136.9
(-3)
P 		522
avr 581.8
(3) 		454.6
(1.3) 		357.5
(0) 		285.9
(-0.9) 		389.1
(0.4) 		131.3
(-3) 		451
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187318_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7779
Sequence Type Exemplar sequence
Representative Public ID F48E8.1 NCBI
Target Description F48E8.1 /REP_DB=WormBase Gene ID /WP=CE01953 /TR=SW:Q09566 /GB=AAC46538.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065766(10), NM_171866(9), NM_065765(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065766 NCBI Caenorhabditis elegans LONg family member (lon-1) (lon-1) mRNA, complete cds. 10/11 None
NM_171866 NCBI Caenorhabditis elegans LONg family member (lon-1) (lon-1) mRNA, complete cds. 9/11 None
NM_065765 NCBI Caenorhabditis elegans LONg family member (lon-1) (lon-1) mRNA, complete cds. 11/11 None
SNAP00000003644 ENSEMBL cdna:SNAP chromosome:CEL140:III:5474477:5477770:1 10/11 None
F48E8.1b ENSEMBL cdna:known chromosome:CEL140:III:5474434:5477969:1 gene:F48E8.1 10/11 None
F48E8.1c ENSEMBL cdna:known chromosome:CEL140:III:5474477:5477952:1 gene:F48E8.1 9/11 None
F48E8.1a ENSEMBL cdna:known chromosome:CEL140:III:5474477:5477951:1 gene:F48E8.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000003645 7/11 Cross Hyb Matching Probes None
GENEFINDER00000003652 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:5474475-5477769(+) 100.0 100.0
Public Domain and Genome References
Ensembl F48E8.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC46538 Long protein 1, isoform a [Caenorhabditis elegans] ref|NP_498166.1| LONg family member (lon-1) [Caenorhabditis elegans] sp|Q09566|LON1_CAEEL Protein lon-1 precursor 1.0E-156
blast AAK95879 Long protein 1, isoform b [Caenorhabditis elegans] ref|NP_498167.1| LONg family member (lon-1) [Caenorhabditis elegans] 1.0E-150
blast AAC46538 Long protein 1, isoform a [Caenorhabditis elegans] ref|NP_498166.1| LONg family member (lon-1) [Caenorhabditis elegans] sp|Q09566|LON1_CAEEL Protein lon-1 precursor 1.0E-148
blast AAK95879 Long protein 1, isoform b [Caenorhabditis elegans] ref|NP_498167.1| LONg family member (lon-1) [Caenorhabditis elegans] 1.0E-148
blast CAE61354 Hypothetical protein CBG05195 [Caenorhabditis briggsae] 1.0E-144
Protein Domains
Database ID Description E-Value
Pfam IPR001283 EMBL-EBI Allergen V5/Tpx-1 related 5.8E-45
Pfam IPR001283 EMBL-EBI Allergen V5/Tpx-1 related 1.7E-36
Sequence
Target Sequence 
>C. ELEGANS:187318_AT
cacgcggacacctgtgatttccgtcactcccgtggacgcatcaacgtcggcgaaaacatc
tgggcggcgccgtacagtaactattctgacgctatctcgatctggttcaacgaggtccat
aatccaagatgtggatgtaaccatgcctataaacactgttgcggtcactatgttcaggtt
gtctgggcaaagacgaatctcgtcggatgcggcttctcccgttgccgtgacgttcagggt
gtctggggacgtggacatcgtaacgtattcgtctgtcactacaatccacagggaaatacg
gtattcgtcactgcgagaggacagctttatgcgatgccagctttcacctgggcttccggt
gacaatggaaaatgctccaactgcccagcaaatgctccagcctgctaccaagggctctgc
tacatgccaaagaactacgaggctccgactacaacaacggaatccaccacaacatctaca
acaactgaggaaccaacgacaacttgtgagcccgatgaaccaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CACGCGGACACCTGTGATTTCCGTC 247 201 358 Antisense
ACGTCGGCGAAAACATCTGGGCGGC 283 93 401 Antisense
GGCGCCGTACAGTAACTATTCTGAC 221 543 423 Antisense
TGACGCTATCTCGATCTGGTTCAAC 164 571 444 Antisense
GCGGTCACTATGTTCAGGTTGTCTG 177 293 518 Antisense
CCCGTTGCCGTGACGTTCAGGGTGT 649 237 575 Antisense
GTATTCGTCTGTCACTACAATCCAC 265 453 622 Antisense
GAGGACAGCTTTATGCGATGCCAGC 180 405 674 Antisense
CTTTCACCTGGGCTTCCGGTGACAA 168 223 698 Antisense
CTACCAAGGGCTCTGCTACATGCCA 349 225 762 Antisense
CAACTTGTGAGCCCGATGAACCAGA 390 189 857 Antisense
Sequence Source Affymetrix Proprietary Database