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[187315_s_at]

Description B0228.2 /REP_DB=WormBase Gene ID /WP=CE01743 /TR=SW:Q09221 /GB=AAC38806.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 32      6grp_high1_10_10 > 23      4grp_highest_8_8 > 41      4grp_high_8_8 > 49      6grp_highest_nonorm > 21      6grp_total > 13      4grp_total > 54      6grp_highest_sub > De      6grp_high1_sub > 02      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1147
1 2 3 4 range
A 1397.7
(3)
P 		752
(-0.4)
P 		695.3
(-0.7)
P 		250.8
(-3)
P 		1147
B 1210.4
(3)
P 		787.6
(0.1)
P 		608.4
(-1.1)
P 		333.2
(-3)
P 		877
C 1151.6
(3)
P 		738.2
(0.2)
P 		732.1
(0.1)
P 		269.8
(-3)
P 		882
avr 1253.2
(3) 		759.3
(-0.1) 		678.6
(-0.6) 		284.6
(-3) 		969

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1727
1 2 3 4 5 6 range
A 1515.9
(0.3)
P 		2243.2
(3)
P 		868.1
(-2.1)
P 		1684.4
(0.9)
P 		948.2
(-1.8)
P 		633
(-3)
P 		1610
B 1401.3
(3)
P 		1405.9
(3)
P 		1048.2
(0.6)
P 		1272.9
(2.1)
P 		807.8
(-1)
P 		516.5
(-3)
P 		889
C 1890
(2.4)
P 		1738.5
(1.8)
P 		2023
(3)
P 		1372.4
(0.3)
P 		891.5
(-1.8)
P 		595
(-3)
P 		1428
avr 1602.4
(2) 		1795.9
(3) 		1313.1
(0.6) 		1443.2
(1.3) 		882.5
(-1.5) 		581.5
(-3) 		1214
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 472
1 2 3 4 range
A 508.9
(3)
P 		163.2
(-1.4)
P 		181.8
(-1.2)
P 		36.7
(-3)
P 		472
B 386
(3)
P 		194.5
(-0.6)
P 		141
(-1.7)
P 		71
(-3)
P 		315
C 381.2
(3)
P 		212.2
(-0)
P 		255.2
(0.7)
P 		45.5
(-3)
P 		336
avr 425.4
(3) 		190
(-0.8) 		192.7
(-0.7) 		51.1
(-3) 		374

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 644
1 2 3 4 5 6 range
A 279.4
(-1.8)
P 		797.8
(3)
P 		189.1
(-2.7)
P 		427.2
(-0.5)
P 		286.1
(-1.8)
P 		154.1
(-3)
P 		644
B 487.6
(3)
P 		468
(2.6)
P 		254.5
(-1.7)
P 		337.2
(-0.1)
P 		328.2
(-0.2)
P 		192.6
(-3)
P 		295
C 700.5
(3)
P 		530.5
(1)
P 		514.9
(0.8)
P 		276
(-2.1)
P 		288.5
(-1.9)
P 		199.2
(-3)
P 		501
avr 489.2
(1.4) 		598.8
(3) 		319.5
(-1) 		346.8
(-0.6) 		300.9
(-1.3) 		182
(-3) 		417
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 187315_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4560
Sequence Type Exemplar sequence
Representative Public ID B0228.2 NCBI
Target Description B0228.2 /REP_DB=WormBase Gene ID /WP=CE01743 /TR=SW:Q09221 /GB=AAC38806.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063224(11), NM_063222(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063224 NCBI Caenorhabditis elegans Temporarily Assigned Gene name family member (tag-308) (tag-308) mRNA, complete cds. 11/11 None
NM_063222 NCBI Caenorhabditis elegans Temporarily Assigned Gene name family member (tag-308) (tag-308) mRNA, complete cds. 11/11 A 
SNAP00000020602 ENSEMBL cdna:SNAP chromosome:CEL140:II:7743889:7752348:1 11/11 None
GENEFINDER00000020737 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:7725004:7752348:1 11/11 None
B0228.4c ENSEMBL cdna:known chromosome:CEL140:II:7724818:7752635:1 gene:B0228.4 11/11 None
B0228.4a ENSEMBL cdna:known chromosome:CEL140:II:7724818:7752348:1 gene:B0228.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
174250 4 2 NM_001026713 174824_at (11)
NM_063222 187315_s_at (11)
NM_063224 187308_at (9), 187315_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7745959-7752345(+) 99.29 100.0
Public Domain and Genome References
Ensembl B0228.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAU87832 Temporarily assigned gene name protein 308, isoform c [Caenorhabditis elegans] ref|NP_495625.2| Temporarily Assigned Gene name family member (tag-308) [Caenorhabditis elegans] 0.0
blast T29042 hypothetical protein B0228.3 - Caenorhabditis elegans 0.0
blast AAU87831 Temporarily assigned gene name protein 308, isoform b [Caenorhabditis elegans] ref|NP_871900.2| Temporarily Assigned Gene name family member (tag-308) [Caenorhabditis elegans] 0.0
blast AAC38806 Temporarily assigned gene name protein 308, isoform a [Caenorhabditis elegans] ref|NP_495623.2| Temporarily Assigned Gene name family member (tag-308) [Caenorhabditis elegans] sp|Q09221|YP74_CAEEL Hypothetical protein B0228.4 in chromosome II 0.0
blast AAU87833 Temporarily assigned gene name protein 308, isoform d [Caenorhabditis elegans] ref|NP_001021884.1| Temporarily Assigned Gene name family member (tag-308) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR010734 EMBL-EBI Copine 1.4E-70
Pfam IPR010734 EMBL-EBI Copine 1.4E-70
Pfam IPR010734 EMBL-EBI Copine 1.4E-70
Pfam IPR010734 EMBL-EBI Copine 1.4E-70
Sequence
Target Sequence 
>C. ELEGANS:187315_S_AT
gaccacttcacactattgatccagctgaaatgaatccatatcaacaggtcattcaaattg
tcggaaaaactctttcatctttcgatgccgatggacaaattcctgcctacggttttggtg
acgaagagttcacggatcatggaatctttaacattgcggaaagatacgatcttgaaaagg
attgtaacggatttgaagaagtacttcgagtttacaacgaagttaccccaaccatcgaaa
tgagcggaccaactaactttgtgccattgattgatcgtgcaatcgaaatctgtaaggaaa
aacattcctatcacattttggttatcgttgctgacggacaggtcaccaatgaaaagatca
atcagaaggcaatcgcagccgcttcacattatccattgagtattatcatggttggtgttg
gagatggtccatggaatatgatgggaagattcgatgataacattccaaagagactatttg
ataacttccatttcgttgatttccataaggtaatgttcaatgcaccaaatgccgacgcat
cttttgcattgaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACCACTTCACACTATTGATCCAGC 682 383 4751 Antisense
GATGCCGATGGACAAATTCCTGCCT 464 415 4834 Antisense
TTCCTGCCTACGGTTTTGGTGACGA 679 697 4850 Antisense
TACAACGAAGTTACCCCAACCATCG 181 639 4963 Antisense
GAGCGGACCAACTAACTTTGTGCCA 359 385 4992 Antisense
GGTTATCGTTGCTGACGGACAGGTC 609 503 5070 Antisense
TCAGAAGGCAATCGCAGCCGCTTCA 114 625 5112 Antisense
AGCCGCTTCACATTATCCATTGAGT 186 83 5127 Antisense
GATAACTTCCATTTCGTTGATTTCC 16 423 5230 Antisense
GTTCAATGCACCAAATGCCGACGCA 672 445 5265 Antisense
TGCCGACGCATCTTTTGCATTGAAT 279 585 5280 Antisense
Sequence Source Affymetrix Proprietary Database