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[187308_at]

Description B0228.3 /REP_DB=WormBase Gene ID /WP=CE01744 /TR=SW:Q09222 /GB=AAC38807.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 35      4grp_total > 55      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 63
1 2 3 4 range
A 2.5
(-3)
A 		3.1
(-2.6)
A 		10.8
(3)
A 		8.7
(1.5)
A 		8
B 64.8
(3)
A 		7.3
(-2.6)
A 		14.4
(-1.9)
A 		3.2
(-3)
A 		62
C 47.6
(3)
A 		3.1
(-2.9)
A 		3.4
(-2.8)
A 		2.2
(-3)
A 		45
avr 38.3
(3) 		4.5
(-3) 		9.5
(-2.1) 		4.7
(-3) 		34

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 28
1 2 3 4 5 6 range
A 10.5
(-1.2)
A 		29.5
(3)
A 		4.2
(-2.6)
A 		2.5
(-3)
A 		2.4
(-3)
A 		6.2
(-2.2)
A 		27
B 2.1
(-2.2)
A 		2.8
(-1.5)
A 		7.3
(3)
A 		1.7
(-2.6)
A 		1.3
(-3)
A 		1.4
(-2.9)
A 		6
C 15.6
(3)
A 		2.6
(-2.9)
A 		13.4
(2)
A 		2.4
(-3)
A 		8.4
(-0.3)
A 		3.7
(-2.4)
A 		13
avr 9.4
(1.6) 		11.6
(3) 		8.3
(0.9) 		2.2
(-3) 		4
(-1.9) 		3.8
(-2) 		9
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 20
1 2 3 4 range
A 0.9
(-2.4)
A 		0.7
(-3)
A 		2.8
(3)
A 		1.3
(-1.3)
A 		2
B 20.7
(3)
A 		1.8
(-2.7)
A 		3.3
(-2.2)
A 		0.7
(-3)
A 		20
C 15.8
(3)
A 		0.9
(-2.8)
A 		1.2
(-2.7)
A 		0.4
(-3)
A 		15
avr 12.5
(3) 		1.1
(-2.8) 		2.4
(-2.2) 		0.8
(-3) 		12

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 10
1 2 3 4 5 6 range
A 1.9
(-2.2)
A 		10.5
(3)
A 		0.9
(-2.8)
A 		0.6
(-3)
A 		0.7
(-2.9)
A 		1.5
(-2.5)
A 		10
B 0.7
(-1.7)
A 		0.9
(-0.9)
A 		1.8
(3)
A 		0.4
(-3)
A 		0.5
(-2.6)
A 		0.5
(-2.6)
A 		1
C 5.8
(3)
A 		0.8
(-2.7)
A 		3.4
(0.3)
A 		0.5
(-3)
A 		2.7
(-0.5)
A 		1.2
(-2.2)
A 		5
avr 2.8
(0.8) 		4.1
(3) 		2
(-0.5) 		0.5
(-3) 		1.3
(-1.7) 		1.1
(-2) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187308_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4373
Sequence Type Exemplar sequence
Representative Public ID B0228.3 NCBI
Target Description B0228.3 /REP_DB=WormBase Gene ID /WP=CE01744 /TR=SW:Q09222 /GB=AAC38807.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063224(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063224 NCBI Caenorhabditis elegans Temporarily Assigned Gene name family member (tag-308) (tag-308) mRNA, complete cds. 9/11 None
SNAP00000020602 ENSEMBL cdna:SNAP chromosome:CEL140:II:7743889:7752348:1 10/11 None
GENEFINDER00000020737 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:7725004:7752348:1 10/11 None
B0228.4c ENSEMBL cdna:known chromosome:CEL140:II:7724818:7752635:1 gene:B0228.4 9/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
174250 4 1 NM_001026713 174824_at (11)
NM_063222 187315_s_at (11)
NM_063224 187308_at (9), 187315_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7731718-7745793(+) 100.0 100.0
Public Domain and Genome References
Ensembl B0228.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAU87832 Temporarily assigned gene name protein 308, isoform c [Caenorhabditis elegans] ref|NP_495625.2| Temporarily Assigned Gene name family member (tag-308) [Caenorhabditis elegans] 0.0
blast T29042 hypothetical protein B0228.3 - Caenorhabditis elegans 0.0
blast AAU87831 Temporarily assigned gene name protein 308, isoform b [Caenorhabditis elegans] ref|NP_871900.2| Temporarily Assigned Gene name family member (tag-308) [Caenorhabditis elegans] 0.0
blast T29043 hypothetical protein B0228.2 - Caenorhabditis elegans 0.0
blast CAE67471 Hypothetical protein CBG12974 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR010734 EMBL-EBI Copine 1.4E-70
Pfam IPR010734 EMBL-EBI Copine 1.4E-70
Pfam IPR010734 EMBL-EBI Copine 1.4E-70
Sequence
Target Sequence 
>C. ELEGANS:187308_AT
ccgtttcgatttgctctttggatactgttcactctaggaaatctgagataccacctacat
attctgtgagcaaagtgttgaccgagaagcgtgtacttcgtgagacgtggcgagtgatag
agagtggtgatgtgcgattcaacgctgtcatcaatttgcatagatacagtttatcgaaac
cgacactggcacaagagacggtgcttcgggaggttgtacgaattgcagctcaaccattat
tcatatcagcaggtgatatcgaaagtgatgttgtttggagtacccatcaattggagaaaa
cattcgtgagagaagaaattgatagaaccatcattggagcaagaaaaggcgaaaatatca
agaagaagtttgaagaagctggtgatgaaaaagtgaagttaaaggttgaactgattggag
gaaatcttccaaacgagatttgcgatatttcttggaaaatcccaagacttggaggaaagg
tgaagctggaaaccgaagagtttgagtttgatgagtgcttgttcctatgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCGTTTCGATTTGCTCTTTGGATAC 138 265 12611 Antisense
GATACTGTTCACTCTAGGAAATCTG 36 423 12631 Antisense
CTGAGATACCACCTACATATTCTGT 354 239 12653 Antisense
GCGTGTACTTCGTGAGACGTGGCGA 154 289 12699 Antisense
ATGTGCGATTCAACGCTGTCATCAA 269 49 12740 Antisense
GAAACCGACACTGGCACAAGAGACG 58 359 12786 Antisense
AGAGACGGTGCTTCGGGAGGTTGTA 83 63 12804 Antisense
GGAGGTTGTACGAATTGCAGCTCAA 420 515 12819 Antisense
GCAGCTCAACCATTATTCATATCAG 458 313 12835 Antisense
GTTGTTTGGAGTACCCATCAATTGG 86 437 12880 Antisense
GTTTGATGAGTGCTTGTTCCTATGG 362 451 13116 Antisense
Sequence Source Affymetrix Proprietary Database